MNMG_THEMA
ID MNMG_THEMA Reviewed; 629 AA.
AC Q9WYA1;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129}; OrderedLocusNames=TM_0263;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- FUNCTION: NAD-binding protein involved in the addition of a
CC carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC Rule:MF_00129}.
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DR EMBL; AE000512; AAD35351.1; -; Genomic_DNA.
DR PIR; F72400; F72400.
DR RefSeq; NP_228076.1; NC_000853.1.
DR AlphaFoldDB; Q9WYA1; -.
DR SMR; Q9WYA1; -.
DR STRING; 243274.THEMA_03410; -.
DR EnsemblBacteria; AAD35351; AAD35351; TM_0263.
DR KEGG; tma:TM0263; -.
DR PATRIC; fig|243274.18.peg.666; -.
DR eggNOG; COG0445; Bacteria.
DR InParanoid; Q9WYA1; -.
DR OMA; FRPGYAI; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IBA:GO_Central.
DR GO; GO:0030488; P:tRNA methylation; IBA:GO_Central.
DR GO; GO:0002098; P:tRNA wobble uridine modification; IBA:GO_Central.
DR Gene3D; 1.10.150.570; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR HAMAP; MF_00129; MnmG_GidA; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR004416; MnmG.
DR InterPro; IPR002218; MnmG-rel.
DR InterPro; IPR020595; MnmG-rel_CS.
DR InterPro; IPR026904; MnmG_C.
DR InterPro; IPR044920; MnmG_C_subdom.
DR InterPro; IPR040131; MnmG_N.
DR PANTHER; PTHR11806; PTHR11806; 1.
DR PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR Pfam; PF01134; GIDA; 1.
DR Pfam; PF13932; GIDA_C; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR TIGRFAMs; TIGR00136; gidA; 1.
DR PROSITE; PS01280; GIDA_1; 1.
DR PROSITE; PS01281; GIDA_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; FAD; Flavoprotein; NAD; Reference proteome; tRNA processing.
FT CHAIN 1..629
FT /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT enzyme MnmG"
FT /id="PRO_0000117202"
FT BINDING 18..23
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT BINDING 280..294
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ SEQUENCE 629 AA; 71040 MW; 0D8052BB809C2999 CRC64;
MIGLRPEDDR VYDVIVVGAG HAGIEAALAA ARMGFRVLVL TVNPDTVGWA PCNPAIGGPA
KGVVVREIDA LGGEMAKTTD ETMINVRMLN VSKGPAVRAL RAQIDKISYS RTMKRKLETN
PNIVLRHGIV ERILTEKGRV KGVVDNYGID YLGKAVIVTT GTFLRGKIFI GRSTFPAGRM
GEFPATKLTE SLIELGFEVG RFKTGTPARV LKRSINFSVM ERQDTSDEPL AFSFFDEPRV
LPKDYPCWLT RTNPETHSII KQYLEFSPLY GTVKLIEGIG PRYCPSIEDK VVKFKDKESH
QVFVEPEGRD TEEYYLNGLS TSLPYEAQIK MIRSVKGLEN AIVTRPAYAI EYDYIDPRQL
YPTLESKLVE NLYFAGQVNG TSGYEEAAGQ GIIAGINAAL KLRGEPPLIL KRSEAYIGVL
IDDLVTKGVD EPYRLLTSRA EYRLLLRHDN AHLRLAKYGY RVGLIPKWFY EKVLSLERRI
NEEIERLKKV IVKPSDRVND LLTSLRTSPL KEPVSFYQLL KRPQLSYSAL KFLDPNPIDD
PEVVEQVEIN VKYEGYIQKM FEEVAVFEKY ENYEIPHDLD YDAVPNLSTE ARDKLKKIRP
RSIGQAMRIP GINPSDISNL IIYLDRKKQ
