ID   MNMG_WOLWR              Reviewed;         644 AA.
AC   C0R430;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG {ECO:0000255|HAMAP-Rule:MF_00129};
DE   AltName: Full=Glucose-inhibited division protein A {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Name=mnmG {ECO:0000255|HAMAP-Rule:MF_00129};
GN   Synonyms=gidA {ECO:0000255|HAMAP-Rule:MF_00129};
GN   OrderedLocusNames=WRi_009600;
OS   Wolbachia sp. subsp. Drosophila simulans (strain wRi).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Anaplasmataceae; Wolbachieae; Wolbachia; unclassified Wolbachia.
OX   NCBI_TaxID=66084;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=wRi;
RX   PubMed=19307581; DOI=10.1073/pnas.0810753106;
RA   Klasson L., Westberg J., Sapountzis P., Naeslund K., Lutnaes Y.,
RA   Darby A.C., Veneti Z., Chen L., Braig H.R., Garrett R., Bourtzis K.,
RA   Andersson S.G.;
RT   "The mosaic genome structure of the Wolbachia wRi strain infecting
RT   Drosophila simulans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:5725-5730(2009).
CC   -!- FUNCTION: NAD-binding protein involved in the addition of a
CC       carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of
CC       certain tRNAs, forming tRNA-cmnm(5)s(2)U34. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00129};
CC   -!- SUBUNIT: Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
CC       {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00129}.
CC   -!- SIMILARITY: Belongs to the MnmG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00129}.
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DR   EMBL; CP001391; ACN95672.1; -; Genomic_DNA.
DR   RefSeq; WP_007548926.1; NZ_MKIF01000070.1.
DR   AlphaFoldDB; C0R430; -.
DR   SMR; C0R430; -.
DR   STRING; 66084.WRi_009600; -.
DR   EnsemblBacteria; ACN95672; ACN95672; WRi_009600.
DR   KEGG; wri:WRi_009600; -.
DR   HOGENOM; CLU_007831_2_2_5; -.
DR   OMA; FRPGYAI; -.
DR   Proteomes; UP000001293; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0002098; P:tRNA wobble uridine modification; IEA:InterPro.
DR   Gene3D; 1.10.150.570; -; 1.
DR   Gene3D; 3.50.50.60; -; 2.
DR   HAMAP; MF_00129; MnmG_GidA; 1.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR004416; MnmG.
DR   InterPro; IPR002218; MnmG-rel.
DR   InterPro; IPR020595; MnmG-rel_CS.
DR   InterPro; IPR026904; MnmG_C.
DR   InterPro; IPR044920; MnmG_C_subdom.
DR   InterPro; IPR040131; MnmG_N.
DR   PANTHER; PTHR11806; PTHR11806; 1.
DR   PANTHER; PTHR11806:SF0; PTHR11806:SF0; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   Pfam; PF13932; GIDA_C; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
DR   TIGRFAMs; TIGR00136; gidA; 1.
DR   PROSITE; PS01280; GIDA_1; 1.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; FAD; Flavoprotein; NAD; tRNA processing.
FT   CHAIN           1..644
FT                   /note="tRNA uridine 5-carboxymethylaminomethyl modification
FT                   enzyme MnmG"
FT                   /id="PRO_1000122760"
FT   BINDING         10..15
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
FT   BINDING         273..287
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00129"
SQ   SEQUENCE   644 AA;  71650 MW;  F0BECE966C4B77FE CRC64;
     MHKYDVVVVG GGHAGCEAAA AAARLGTNTL LITHKISTIG EMSCNPAIGG VAKGVVVREV
     DALDGIMGRA IDRASIHSVI LNSSRGAAVW GPRAQADRKL YKQAIQEIIL NYNNLTVKEE
     SVDDFLIESN NNGELCIKAV ITSSGEHILT GKVVLTTGTF LQGVIHIGEQ TTPSGRMGDK
     PAVELANTLN KYDFRLGRLR TGTPPRLDRG TINWSILQEQ VGDNPPVPFS YLTEKINQPQ
     VSCFITHTNE NTHKIIRENL HRSASSYLSD VVAPRYCPSI EVKVKKFAEK SSHQIFLEPE
     GLDDDTVYPN GISNSLPIEV QREMIKSIKG LENAEILRPG YAVEYDYIDP RELFHTLETK
     KVKGLYFAGQ INGTTGYEEA AGQGIIAGIN AALSAFEKKE SFVLHRTDSY IGVMIDDLVT
     KGVIEPYRLF TSRAEYRLAI RSDNADRRLT QKGYDISLVS HERYSVLQNK LESIKQLEEK
     LGSLTITPEQ LRSYGIKISY DGIRKTALDL LSYPNIDWNK LQEIWPELSS VTRWNDKMGH
     TKADNRAKNE ICEAVEIEAK YKPYLIRQEA DMKFLREEIN TQIPIDFNYS QVKGLSSEVI
     EKLQTIKPAT IGIAKQIQGI TPAAIVSILV YLRNRKTKVA ANFV