MNN11_YEAST
ID MNN11_YEAST Reviewed; 422 AA.
AC P46985; D6VW07; E9P8Z0;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Probable alpha-1,6-mannosyltransferase MNN11;
DE EC=2.4.1.-;
DE AltName: Full=Mannan polymerase II complex MNN11 subunit;
DE Short=M-Pol II subunit MNN11;
GN Name=MNN11; OrderedLocusNames=YJL183W; ORFNames=J0425;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 298.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP PROTEIN SEQUENCE OF N-TERMINUS, SUBUNIT, SUBCELLULAR LOCATION, AND ACTIVITY
RP OF M-POL II COMPLEX.
RX PubMed=9430634; DOI=10.1093/emboj/17.2.423;
RA Jungmann J., Munro S.;
RT "Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with
RT alpha-1,6-mannosyltransferase activity.";
RL EMBO J. 17:423-434(1998).
RN [5]
RP FUNCTION, AND SUBUNIT.
RX PubMed=10037752; DOI=10.1074/jbc.274.10.6579;
RA Jungmann J., Rayner J.C., Munro S.;
RT "The Saccharomyces cerevisiae protein Mnn10p/Bed1p is a subunit of a Golgi
RT mannosyltransferase complex.";
RL J. Biol. Chem. 274:6579-6585(1999).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for synthesis of full-length mannan chains.
CC {ECO:0000269|PubMed:10037752}.
CC -!- FUNCTION: The M-Pol II complex possesses alpha-1,6-mannosyltransferase
CC activity and is probably involved in the elongation of the mannan
CC backbone of N-linked glycans on cell wall and periplasmic proteins.
CC {ECO:0000269|PubMed:10037752}.
CC -!- SUBUNIT: Component of the M-Pol II complex composed of ANP1, MNN9,
CC MNN10, MNN11 and HOC1. {ECO:0000269|PubMed:10037752,
CC ECO:0000269|PubMed:9430634}.
CC -!- INTERACTION:
CC P46985; P32629: ANP1; NbExp=3; IntAct=EBI-11052, EBI-2595;
CC P46985; P47124: HOC1; NbExp=2; IntAct=EBI-11052, EBI-8430;
CC P46985; P50108: MNN10; NbExp=5; IntAct=EBI-11052, EBI-11043;
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, cis-Golgi network membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:9430634}; Single-pass
CC type II membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:9430634}.
CC -!- MISCELLANEOUS: Present with 3480 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 34 family.
CC {ECO:0000305}.
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DR EMBL; Z49458; CAA89478.1; -; Genomic_DNA.
DR EMBL; AY692909; AAT92928.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08623.2; -; Genomic_DNA.
DR PIR; S56966; S56966.
DR RefSeq; NP_012352.2; NM_001181616.2.
DR AlphaFoldDB; P46985; -.
DR BioGRID; 33579; 624.
DR ComplexPortal; CPX-1839; alpha-1,6-mannosyltransferase complex, M-Pol II variant.
DR DIP; DIP-914N; -.
DR IntAct; P46985; 21.
DR STRING; 4932.YJL183W; -.
DR CAZy; GT34; Glycosyltransferase Family 34.
DR iPTMnet; P46985; -.
DR SwissPalm; P46985; -.
DR MaxQB; P46985; -.
DR PaxDb; P46985; -.
DR PRIDE; P46985; -.
DR EnsemblFungi; YJL183W_mRNA; YJL183W; YJL183W.
DR GeneID; 853256; -.
DR KEGG; sce:YJL183W; -.
DR SGD; S000003719; MNN11.
DR VEuPathDB; FungiDB:YJL183W; -.
DR eggNOG; KOG4748; Eukaryota.
DR HOGENOM; CLU_021434_1_0_1; -.
DR InParanoid; P46985; -.
DR OMA; YIRWIQE; -.
DR BioCyc; MetaCyc:G3O-31617-MON; -.
DR BioCyc; YEAST:G3O-31617-MON; -.
DR PRO; PR:P46985; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P46985; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; IBA:GO_Central.
DR GO; GO:0000136; C:mannan polymerase complex; IDA:UniProtKB.
DR GO; GO:0000009; F:alpha-1,6-mannosyltransferase activity; IDA:UniProtKB.
DR GO; GO:0000032; P:cell wall mannoprotein biosynthetic process; IDA:UniProtKB.
DR GO; GO:0006486; P:protein glycosylation; IDA:SGD.
DR GO; GO:0006487; P:protein N-linked glycosylation; IMP:SGD.
DR Gene3D; 3.90.550.10; -; 1.
DR InterPro; IPR008630; Glyco_trans_34.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR PANTHER; PTHR31306; PTHR31306; 1.
DR Pfam; PF05637; Glyco_transf_34; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycosyltransferase; Golgi apparatus; Membrane;
KW Reference proteome; Signal-anchor; Transferase; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..422
FT /note="Probable alpha-1,6-mannosyltransferase MNN11"
FT /id="PRO_0000215165"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..422
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CONFLICT 298
FT /note="D -> G (in Ref. 1; CAA89478)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 422 AA; 47859 MW; B1764ADBC19F9E58 CRC64;
MAIKPRTKGK TYSSRSVGSQ WFNRLGFKQN KYGTCKFLSI ITAFVFILYF FSNRFYPISR
SAGASYSPSH GLYINEIPAS SRLIYPHVEH VPVLKQMTVR GLYITRLEVD GSKRLILKPE
ENALTDEEKK KTTDQILLVK HSFLDHGKLV YRKSNDAPEV VVVTLIDFEN YELETIIQIV
QNRVDYAQKH QYGVYIRWIQ EFLPVLENQN LAESYEFIKP LVIRAAMHAF PTAKYIHFVD
QDALLMNLDL SLQKYLLDPK IMDLALLKNV PVVANSNIKT YNHFEYSSAK IIIPHDADGN
IDASSFVIAN DFYGKALIDY LNDPLLRNFP WDNTGDKLSA AIGHILQWHP TLLGKTAIVI
PKVLASQYDA SLDQEGESGN GASNGDVYHY NEGDLAASFK GCRSRGTCAS EIGHMYQKIK
KS
