ID   MNR1_CAPAN              Reviewed;         314 AA.
AC   B2X050;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   03-AUG-2022, entry version 47.
DE   RecName: Full=(+)-neomenthol dehydrogenase;
DE            EC=1.1.1.208;
DE   AltName: Full=Menthone:neomenthol reductase 1;
DE            Short=CaMNR1;
GN   Name=MNR1;
OS   Capsicum annuum (Capsicum pepper).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Capsiceae; Capsicum.
OX   NCBI_TaxID=4072;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBUNIT, INDUCTION, TISSUE
RP   SPECIFICITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=cv. Nockwang;
RX   PubMed=18599651; DOI=10.1104/pp.108.119461;
RA   Choi H.W., Lee B.G., Kim N.H., Park Y., Lim C.W., Song H.K., Hwang B.K.;
RT   "A role for a menthone reductase in resistance against microbial pathogens
RT   in plants.";
RL   Plant Physiol. 148:383-401(2008).
CC   -!- FUNCTION: Involved in basal resistance against pathogens.
CC       {ECO:0000269|PubMed:18599651}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(+)-neomenthol + NADP(+) = (1R,4S)-menthone + H(+) + NADPH;
CC         Xref=Rhea:RHEA:23812, ChEBI:CHEBI:15378, ChEBI:CHEBI:15402,
CC         ChEBI:CHEBI:15410, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.208;
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=35.1 uM for menthone {ECO:0000269|PubMed:18599651};
CC         KM=21.7 uM for NADPH {ECO:0000269|PubMed:18599651};
CC         KM=113.2 uM for neomenthol {ECO:0000269|PubMed:18599651};
CC         KM=76.7 uM for NADP {ECO:0000269|PubMed:18599651};
CC       pH dependence:
CC         Optimum pH is 9.0 for the forward reaction and 7.5 for the reverse
CC         reaction. {ECO:0000269|PubMed:18599651};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:18599651}.
CC   -!- TISSUE SPECIFICITY: Expressed in flowers and red fruit tissues. Not
CC       detected in leaves, stems, roots or green fruits.
CC       {ECO:0000269|PubMed:18599651}.
CC   -!- INDUCTION: Strongly induced by inoculation with an avirulent strain of
CC       pathogen, but not with a virulent strain. Induced by salicylic acid,
CC       ethylene, abscisic acid and drought. Not induced by cold, wounding or
CC       H(2)O(2). {ECO:0000269|PubMed:18599651}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; EF576664; ABU54321.1; -; mRNA.
DR   RefSeq; NP_001311494.1; NM_001324565.1.
DR   AlphaFoldDB; B2X050; -.
DR   SMR; B2X050; -.
DR   EnsemblPlants; PHT74475; PHT74475; T459_21752.
DR   GeneID; 107839158; -.
DR   Gramene; PHT74475; PHT74475; T459_21752.
DR   KEGG; cann:107839158; -.
DR   OMA; KECIETN; -.
DR   BRENDA; 1.1.1.208; 1169.
DR   Proteomes; UP000189700; Genome assembly.
DR   GO; GO:0047501; F:(+)-neomenthol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   CDD; cd05324; carb_red_PTCR-like_SDR_c; 1.
DR   InterPro; IPR045313; CBR1-like.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   NADP; Oxidoreductase; Plant defense.
FT   CHAIN           1..314
FT                   /note="(+)-neomenthol dehydrogenase"
FT                   /id="PRO_0000349097"
FT   ACT_SITE        239
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         13..36
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         183
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   314 AA;  34758 MW;  EF20D4706A076826 CRC64;
     MAEKTTSTRY AVVTGGNKGI GYETCRQLAS KGVVVVLTSR DEKKGIEAIE RLKEESNFTD
     EHILFHQLDI MDPASISSLV NLIKTKFGRL DILINNAGIS GVMVEGDVQV LKEILERYIS
     IVFTEDENGE EGGWTKSGPG SVTNYELTKE CIETNYYGAK RMTEAFIPLL QLSNSPRIVN
     VASSMGKLKL LCNKWAIEVL RDADSLTEEK VDQVVNEFLK DFTEKSTESK GWPSYFTAYK
     VSKASLIAYT RVLATKYPNF RINSVCPGYC KTDVNANTGS LTAGEGAESL VNLALLPNDG
     PSGLFFYRKE VTFF