ID   MNS60_MOUSE             Reviewed;         130 AA.
AC   P0DW27;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-AUG-2022, sequence version 1.
DT   03-AUG-2022, entry version 1.
DE   RecName: Full=Ribosome biogenesis inhibitor MINAS-60 {ECO:0000305};
DE            Short=MINAS-60 {ECO:0000303|PubMed:35393574};
GN   Name=Rbm10 {ECO:0000303|PubMed:35393574, ECO:0000312|MGI:MGI:2384310};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=35393574; DOI=10.1038/s41589-022-01003-9;
RA   Cao X., Khitun A., Harold C.M., Bryant C.J., Zheng S.J., Baserga S.J.,
RA   Slavoff S.A.;
RT   "Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint
RT   inhibitor.";
RL   Nat. Chem. Biol. 0:0-0(2022).
CC   -!- FUNCTION: Acts as a late-stage inhibitor of pre-60S ribosome assembly
CC       by preventing pre-60S ribosome export from nucleus.
CC       {ECO:0000250|UniProtKB:P0DW28}.
CC   -!- SUBUNIT: Interacts with 60S ribosome assembly factors GTPBP4 and MRTO4.
CC       {ECO:0000250|UniProtKB:P0DW28}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:P0DW28}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing, Alternative initiation; Named isoforms=4;
CC       Name=Ribosome biogenesis inhibitor MINAS-60;
CC         IsoId=P0DW27-1; Sequence=Displayed;
CC       Name=1;
CC         IsoId=Q99KG3-1; Sequence=External;
CC       Name=2;
CC         IsoId=Q99KG3-2; Sequence=External;
CC       Name=3;
CC         IsoId=Q99KG3-3; Sequence=External;
CC   -!- MISCELLANEOUS: MINAS-60 is the product of an alternative open reading
CC       frame (alt-ORF) of transcripts coding for the RBM10 (AC Q99KG3) protein
CC       (PubMed:35393574). MINAS-60 and RBM10 ORFs are overlapping and are
CC       formed by shifting the reading frame (PubMed:35393574).
CC       {ECO:0000269|PubMed:35393574}.
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DR   EMBL; AL672073; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC004674; -; NOT_ANNOTATED_CDS; mRNA.
DR   MGI; MGI:2384310; Rbm10.
DR   Proteomes; UP000000589; Chromosome X.
PE   2: Evidence at transcript level;
KW   Alternative initiation; Alternative splicing; Nucleus; Reference proteome.
FT   CHAIN           1..130
FT                   /note="Ribosome biogenesis inhibitor MINAS-60"
FT                   /id="PRO_0000456282"
SQ   SEQUENCE   130 AA;  14391 MW;  E6CB409101D5FC24 CRC64;
     MKDEVVVVTG LAAMEPLIVH RMIVERTAAG TMIIETWTTA HTHESMAVRR ASMSMTTHLK
     SKVQRIPTRP PRAPRLSVGG GGGTGTVPLA RQASPETATI GTRTIGPSKG RRRRRRMRRR
     RRRRPVTSSC