MNT4_YEAST
ID MNT4_YEAST Reviewed; 580 AA.
AC P53745; D6W1N4; Q03127;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Probable alpha-1,3-mannosyltransferase MNT4;
DE EC=2.4.1.-;
GN Name=MNT4; OrderedLocusNames=YNR059W; ORFNames=N3514;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-267.
RC STRAIN=ATCC 28383 / FL100 / VTT C-80102;
RX PubMed=10333520; DOI=10.1016/s0378-1119(99)00117-1;
RA Phalip V., Kuhn I., Lemoine Y., Jeltsch J.-M.;
RT "Characterization of the biotin biosynthesis pathway in Saccharomyces
RT cerevisiae and evidence for a cluster containing BIO5, a novel gene
RT involved in vitamer uptake.";
RL Gene 232:43-51(1999).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the MNN1/MNT family. {ECO:0000305}.
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DR EMBL; Z71674; CAA96341.1; -; Genomic_DNA.
DR EMBL; U53467; AAB63969.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10600.1; -; Genomic_DNA.
DR PIR; S63391; S63391.
DR RefSeq; NP_014457.3; NM_001183236.3.
DR AlphaFoldDB; P53745; -.
DR BioGRID; 35885; 42.
DR DIP; DIP-5262N; -.
DR IntAct; P53745; 2.
DR MINT; P53745; -.
DR STRING; 4932.YNR059W; -.
DR CAZy; GT71; Glycosyltransferase Family 71.
DR MaxQB; P53745; -.
DR PaxDb; P53745; -.
DR PRIDE; P53745; -.
DR EnsemblFungi; YNR059W_mRNA; YNR059W; YNR059W.
DR GeneID; 855796; -.
DR KEGG; sce:YNR059W; -.
DR SGD; S000005342; MNT4.
DR VEuPathDB; FungiDB:YNR059W; -.
DR eggNOG; ENOG502RZ48; Eukaryota.
DR GeneTree; ENSGT00940000176340; -.
DR HOGENOM; CLU_015387_0_1_1; -.
DR InParanoid; P53745; -.
DR OMA; GDKEWFW; -.
DR BioCyc; YEAST:G3O-33363-MON; -.
DR PRO; PR:P53745; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53745; protein.
DR GO; GO:0005794; C:Golgi apparatus; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000033; F:alpha-1,3-mannosyltransferase activity; ISS:SGD.
DR GO; GO:0006493; P:protein O-linked glycosylation; IBA:GO_Central.
DR InterPro; IPR022751; Alpha_mannosyltransferase.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR Pfam; PF11051; Mannosyl_trans3; 1.
DR SUPFAM; SSF53448; SSF53448; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Glycosyltransferase; Membrane; Reference proteome;
KW Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..580
FT /note="Probable alpha-1,3-mannosyltransferase MNT4"
FT /id="PRO_0000080561"
FT TOPO_DOM 1..10
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..29
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..580
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT CARBOHYD 132
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 223
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 349
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 50
FT /note="V -> L (in Ref. 3; AAB63969)"
FT /evidence="ECO:0000305"
FT CONFLICT 87
FT /note="S -> G (in Ref. 3; AAB63969)"
FT /evidence="ECO:0000305"
FT CONFLICT 134
FT /note="T -> A (in Ref. 3; AAB63969)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="E -> D (in Ref. 3; AAB63969)"
FT /evidence="ECO:0000305"
FT CONFLICT 254..267
FT /note="LNYPPQELWFLDVK -> PGTELELRPQFTG (in Ref. 3;
FT AAB63969)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 580 AA; 68082 MW; F94C53283887EC62 CRC64;
MVLRIRRIKK LAPLIFTSLL SLIVLFRVYR QYPFSDHFET RREDDRSGNV HCFSRLRQIE
EYEKPELTSK FYEPNRWKSF ISYVTRSRKD VKTVSRSLSN LDLYQKCSKE IRADQDISLL
HSIETKLFPY INFTALNSEQ SHNFWPVHTR FDGTKYRGQV LQFSSENNSF IGTSPIEFKA
SEPFWENWLN SALQRNSKGV VMSVSEYLVA DTIRLIRVLR LLNNSLPIEI VHKSDLHESS
QQLLIAAARE SGSLNYPPQE LWFLDVKDML NDEYLARFKR FSNKWLAITF CSFQIPIFLD
SDTVPFVPLD TLYEIDGFKR TGTLFFKDRS FPTSKLSPLQ VKVLKQIINN SLDVSSDSEQ
GFEILKHNLN DEMAIDAIEA LIFKKQKHYM DSGLVIFDKQ KHFFCLPIAI MLQFSPIQEF
FHGDKEWFWL SLFISKKEFT FYPIEASNVG RLEKPETLES STICSTQLSH TDVYGNLLWL
NGGLSVCKKN CWNYDFTKRK EIAAKYKSVD ELRNYYQSPV KLEAVIIPDV SKSPWSQQSE
CVMYSYCTHY RKGQYGKLIE FTDSQKKYYE KVVELWNKVV
