MNTH_MYCTU
ID MNTH_MYCTU Reviewed; 428 AA.
AC P9WIZ5; L0T844; O05916;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 43.
DE RecName: Full=Divalent metal cation transporter MntH {ECO:0000255|HAMAP-Rule:MF_00221};
DE AltName: Full=Mramp;
GN Name=mntH {ECO:0000255|HAMAP-Rule:MF_00221}; OrderedLocusNames=Rv0924c;
GN ORFNames=MTCY21C12.18c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP FUNCTION AS A TRANSPORTER, BIOPHYSICOCHEMICAL PROPERTIES, AND INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=10477555; DOI=10.1084/jem.190.5.717;
RA Agranoff D., Monahan I.M., Mangan J.A., Butcher P.D., Krishna S.;
RT "Mycobacterium tuberculosis expresses a novel pH-dependent divalent cation
RT transporter belonging to the Nramp family.";
RL J. Exp. Med. 190:717-724(1999).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=Mt103;
RX PubMed=12117920; DOI=10.1128/iai.70.8.4124-4131.2002;
RA Boechat N., Lagier-Roger B., Petit S., Bordat Y., Rauzier J., Hance A.J.,
RA Gicquel B., Reyrat J.M.;
RT "Disruption of the gene homologous to mammalian Nramp1 in Mycobacterium
RT tuberculosis does not affect virulence in mice.";
RL Infect. Immun. 70:4124-4131(2002).
CC -!- FUNCTION: H(+)-stimulated, divalent metal cation uptake system.
CC Transports zinc and iron. Can also interact with manganese and copper.
CC {ECO:0000255|HAMAP-Rule:MF_00221, ECO:0000269|PubMed:10477555}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 5.5-6.5. {ECO:0000269|PubMed:10477555};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Up-regulated in response to iron and copper.
CC {ECO:0000269|PubMed:10477555}.
CC -!- DISRUPTION PHENOTYPE: Disruption impairs growth under conditions of
CC limited iron avaibility, but does not affect virulence in mice.
CC {ECO:0000269|PubMed:12117920}.
CC -!- SIMILARITY: Belongs to the NRAMP family. {ECO:0000255|HAMAP-
CC Rule:MF_00221}.
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DR EMBL; AL123456; CCP43672.1; -; Genomic_DNA.
DR PIR; D70583; D70583.
DR RefSeq; WP_003898649.1; NZ_NVQJ01000001.1.
DR RefSeq; YP_177767.1; NC_000962.3.
DR AlphaFoldDB; P9WIZ5; -.
DR SMR; P9WIZ5; -.
DR STRING; 83332.Rv0924c; -.
DR PaxDb; P9WIZ5; -.
DR DNASU; 885569; -.
DR GeneID; 885569; -.
DR KEGG; mtu:Rv0924c; -.
DR PATRIC; fig|83332.111.peg.1025; -.
DR TubercuList; Rv0924c; -.
DR eggNOG; COG1914; Bacteria.
DR OMA; IATFVNS; -.
DR PhylomeDB; P9WIZ5; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015086; F:cadmium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005384; F:manganese ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0071281; P:cellular response to iron ion; IBA:GO_Central.
DR GO; GO:0034755; P:iron ion transmembrane transport; IMP:MTBBASE.
DR GO; GO:0071421; P:manganese ion transmembrane transport; IMP:MTBBASE.
DR GO; GO:0071578; P:zinc ion import across plasma membrane; IMP:MTBBASE.
DR HAMAP; MF_00221; NRAMP; 1.
DR InterPro; IPR001046; NRAMP_fam.
DR PANTHER; PTHR11706; PTHR11706; 1.
DR Pfam; PF01566; Nramp; 1.
DR PRINTS; PR00447; NATRESASSCMP.
DR TIGRFAMs; TIGR01197; nramp; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Iron; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport; Zinc.
FT CHAIN 1..428
FT /note="Divalent metal cation transporter MntH"
FT /id="PRO_0000212627"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 258..278
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 299..319
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 334..356
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00221"
SQ SEQUENCE 428 AA; 45004 MW; D90CF98756715CD8 CRC64;
MAGEFRLLSH LCSRGSKVGE LAQDTRTSLK TSWYLLGPAF VAAIAYVDPG NVAANVSSGA
QFGYLLLWVI VAANVMAALV QYLSAKLGLV TGRSLPEAIG KRMGRPARLA YWAQAEIVAM
ATDVAEVIGG AIALRIMFNL PLPIGGIITG VVSLLLLTIQ DRRGQRLFER VITALLLVIA
IGFTASFFVV TPPPNAVLGG LAPRFQGTES VLLAAAIMGA TVMPHAVYLH SGLARDRHGH
PDPGPQRRRL LRVTRWDVGL AMLIAGGVNA AMLLVAALNM RGRGDTASIE GAYHAVHDTL
GATIAVLFAV GLLASGLASS SVGAYAGAMI MQGLLHWSVP MLVRRLITLG PALAILTLGF
DPTRTLVLSQ VVLSFGIPFA VLPLVKLTGS PAVMGGDTNH RATTWVGWVV AVMVSLLNVM
LIYLTVTG
