MNTR_ECOLI
ID MNTR_ECOLI Reviewed; 155 AA.
AC P0A9F1; P75787;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Transcriptional regulator MntR;
DE AltName: Full=Manganese transport regulator;
GN Name=mntR; Synonyms=ybiQ; OrderedLocusNames=b0817, JW0801;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RC STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX PubMed=11466284; DOI=10.1128/jb.183.16.4806-4813.2001;
RA Patzer S.I., Hantke K.;
RT "Dual repression by Fe(2+)-Fur and Mn(2+)-MntR of the mntH gene, encoding
RT an NRAMP-like Mn(2+) transporter in Escherichia coli.";
RL J. Bacteriol. 183:4806-4813(2001).
RN [5]
RP FUNCTION.
RX PubMed=21908668; DOI=10.1128/jb.05872-11;
RA Waters L.S., Sandoval M., Storz G.;
RT "The Escherichia coli MntR miniregulon includes genes encoding a small
RT protein and an efflux pump required for manganese homeostasis.";
RL J. Bacteriol. 193:5887-5897(2011).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), SUBUNIT, AND DOMAIN.
RC STRAIN=K12;
RX PubMed=19701940; DOI=10.1002/prot.22541;
RA Tanaka T., Shinkai A., Bessho Y., Kumarevel T., Yokoyama S.;
RT "Crystal structure of the manganese transport regulatory protein from
RT Escherichia coli.";
RL Proteins 77:741-746(2009).
CC -!- FUNCTION: In the presence of manganese, represses expression of mntH
CC and mntS. Up-regulates expression of mntP.
CC {ECO:0000269|PubMed:11466284, ECO:0000269|PubMed:21908668}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19701940}.
CC -!- INTERACTION:
CC P0A9F1; P16433: hycG; NbExp=4; IntAct=EBI-541895, EBI-541977;
CC P0A9F1; P30750: metN; NbExp=4; IntAct=EBI-541895, EBI-541886;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DOMAIN: It contains an N-terminal DNA-binding domain and a metal-
CC binding domain. {ECO:0000269|PubMed:19701940}.
CC -!- SIMILARITY: Belongs to the DtxR/MntR family. {ECO:0000305}.
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DR EMBL; U00096; AAC73904.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35488.1; -; Genomic_DNA.
DR PIR; A64819; A64819.
DR RefSeq; NP_415338.1; NC_000913.3.
DR RefSeq; WP_000091016.1; NZ_STEB01000019.1.
DR PDB; 2H09; X-ray; 2.10 A; A=1-155.
DR PDBsum; 2H09; -.
DR AlphaFoldDB; P0A9F1; -.
DR SMR; P0A9F1; -.
DR BioGRID; 4261205; 12.
DR BioGRID; 849811; 7.
DR DIP; DIP-28083N; -.
DR IntAct; P0A9F1; 10.
DR STRING; 511145.b0817; -.
DR jPOST; P0A9F1; -.
DR PaxDb; P0A9F1; -.
DR PRIDE; P0A9F1; -.
DR EnsemblBacteria; AAC73904; AAC73904; b0817.
DR EnsemblBacteria; BAA35488; BAA35488; BAA35488.
DR GeneID; 67413856; -.
DR GeneID; 945437; -.
DR KEGG; ecj:JW0801; -.
DR KEGG; eco:b0817; -.
DR PATRIC; fig|1411691.4.peg.1461; -.
DR EchoBASE; EB3106; -.
DR eggNOG; COG1321; Bacteria.
DR HOGENOM; CLU_069532_2_0_6; -.
DR InParanoid; P0A9F1; -.
DR OMA; SWDAIDR; -.
DR PhylomeDB; P0A9F1; -.
DR BioCyc; EcoCyc:G6420-MON; -.
DR EvolutionaryTrace; P0A9F1; -.
DR PRO; PR:P0A9F1; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR CollecTF; EXPREG_000007f0; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:2000144; P:positive regulation of DNA-templated transcription, initiation; IEP:EcoCyc.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.10.60.10; -; 1.
DR InterPro; IPR001367; Fe_dep_repressor.
DR InterPro; IPR036421; Fe_dep_repressor_sf.
DR InterPro; IPR022687; HTH_DTXR.
DR InterPro; IPR022689; Iron_dep_repressor.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF02742; Fe_dep_repr_C; 1.
DR Pfam; PF01325; Fe_dep_repress; 1.
DR SMART; SM00529; HTH_DTXR; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF47979; SSF47979; 1.
DR PROSITE; PS50944; HTH_DTXR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Cytoplasm; DNA-binding; Manganese;
KW Reference proteome; Repressor; Transcription; Transcription regulation.
FT CHAIN 1..155
FT /note="Transcriptional regulator MntR"
FT /id="PRO_0000201110"
FT DOMAIN 34..95
FT /note="HTH dtxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00296"
FT HELIX 27..52
FT /evidence="ECO:0007829|PDB:2H09"
FT HELIX 57..64
FT /evidence="ECO:0007829|PDB:2H09"
FT HELIX 68..80
FT /evidence="ECO:0007829|PDB:2H09"
FT STRAND 84..87
FT /evidence="ECO:0007829|PDB:2H09"
FT TURN 88..90
FT /evidence="ECO:0007829|PDB:2H09"
FT STRAND 91..94
FT /evidence="ECO:0007829|PDB:2H09"
FT HELIX 96..119
FT /evidence="ECO:0007829|PDB:2H09"
FT HELIX 123..133
FT /evidence="ECO:0007829|PDB:2H09"
FT HELIX 134..136
FT /evidence="ECO:0007829|PDB:2H09"
FT HELIX 139..147
FT /evidence="ECO:0007829|PDB:2H09"
SQ SEQUENCE 155 AA; 17640 MW; CB316AC7F1BDD106 CRC64;
MSRRAGTPTA KKVTQLVNVE EHVEGFRQVR EAHRRELIDD YVELISDLIR EVGEARQVDM
AARLGVSQPT VAKMLKRLAT MGLIEMIPWR GVFLTAEGEK LAQESRERHQ IVENFLLVLG
VSPEIARRDA EGMEHHVSEE TLDAFRLFTQ KHGAK
