ID   MO2_ARATH               Reviewed;         407 AA.
AC   O81816; Q94C29;
DT   30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Monooxygenase 2 {ECO:0000303|PubMed:10216258};
DE            Short=AtMO2 {ECO:0000303|PubMed:10216258};
DE            EC=1.14.13.-;
GN   Name=MO2 {ECO:0000303|PubMed:10216258};
GN   OrderedLocusNames=At4g38540 {ECO:0000312|Araport:AT4G38540};
GN   ORFNames=F20M13.100 {ECO:0000312|EMBL:AEE86944.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GENE FAMILY, AND
RP   NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=10216258; DOI=10.1016/s0378-1119(99)00073-6;
RA   Aubourg S., Picaud A., Kreis M., Lecharny A.;
RT   "Structure and expression of three src2 homologues and a novel subfamily of
RT   flavoprotein monooxygenase genes revealed by the analysis of a 25kb
RT   fragment from Arabidopsis thaliana chromosome IV.";
RL   Gene 230:197-205(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617198; DOI=10.1038/47134;
RA   Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA   Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA   Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA   de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA   Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA   Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA   Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA   Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA   Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA   Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA   Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA   Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA   Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA   Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA   Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA   Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA   Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA   Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA   Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA   Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA   Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA   Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA   Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA   Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA   Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA   Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA   de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA   Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA   Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA   Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA   Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA   Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA   Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA   Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA   Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA   Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA   O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA   Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA   Martienssen R., McCombie W.R.;
RT   "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL   Nature 402:769-777(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 189-407.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:Q5EXK1};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q5EXK1}.
CC   -!- TISSUE SPECIFICITY: Expressed in seeds, seedlings, roots, leaves,
CC       flowers, pollen and siliques. {ECO:0000269|PubMed:10216258}.
CC   -!- SIMILARITY: Belongs to the 3-hydroxybenzoate 6-hydroxylase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK59785.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AJ007588; CAA07575.1; -; mRNA.
DR   EMBL; AL035540; CAB37510.1; -; Genomic_DNA.
DR   EMBL; AL161593; CAB80518.1; -; Genomic_DNA.
DR   EMBL; CP002687; AEE86944.1; -; Genomic_DNA.
DR   EMBL; AY037200; AAK59785.1; ALT_INIT; mRNA.
DR   EMBL; BT003036; AAO23601.1; -; mRNA.
DR   PIR; T05682; T05682.
DR   RefSeq; NP_195566.1; NM_120015.3.
DR   AlphaFoldDB; O81816; -.
DR   SMR; O81816; -.
DR   STRING; 3702.AT4G38540.1; -.
DR   SwissPalm; O81816; -.
DR   PaxDb; O81816; -.
DR   PRIDE; O81816; -.
DR   ProteomicsDB; 251325; -.
DR   DNASU; 830011; -.
DR   EnsemblPlants; AT4G38540.1; AT4G38540.1; AT4G38540.
DR   GeneID; 830011; -.
DR   Gramene; AT4G38540.1; AT4G38540.1; AT4G38540.
DR   KEGG; ath:AT4G38540; -.
DR   Araport; AT4G38540; -.
DR   TAIR; locus:2121254; AT4G38540.
DR   eggNOG; KOG2614; Eukaryota.
DR   HOGENOM; CLU_009665_10_2_1; -.
DR   InParanoid; O81816; -.
DR   OMA; RWMLGYD; -.
DR   OrthoDB; 521070at2759; -.
DR   PhylomeDB; O81816; -.
DR   PRO; PR:O81816; -.
DR   Proteomes; UP000006548; Chromosome 4.
DR   ExpressionAtlas; O81816; baseline and differential.
DR   GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0002239; P:response to oomycetes; IEP:TAIR.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR044560; MO/PqsH.
DR   PANTHER; PTHR45934; PTHR45934; 1.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..407
FT                   /note="Monooxygenase 2"
FT                   /id="PRO_0000441115"
FT   CONFLICT        242
FT                   /note="N -> D (in Ref. 4; AAK59785)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   407 AA;  45505 MW;  1A2851349B1ADF4F CRC64;
     MEEEGSPDII IVGAGISGLS TAVGLHRLGI RSMVLESSET LRATGFAFTT WFNAWKAMEA
     LGVSQHIRSL HDRLEGWVVG TISAGTPPTE MLFPESEEYE SRCVQRKLLL EALAGELPEE
     TIRFSSKVVH IELSGCYKKV HLSDGTILKT KVLVGCDGVY SVVGKWLGFK NPATTARLAI
     RGLTHFPEGH GFGKRFFQFY GDGVRSGFIP CDHNTVYWFL THTSTDIDEE TNSEILKEFV
     LNKIKDLPEN IKNVVETTDL DSMVMSQLKY RPPWELLWSN ITKDNVCVAG DALHPMTPDI
     GQGGCSAMED GVILARCLGE AIKAKSLKGE TEENEEEGYK RIEEGLKKYA GERKWRSIDL
     ITTAYTVGFI QQSRGKWMNM FRDRFLSSYL SRMLLKKSHF DCGSLVP