ID   MO3_ARATH               Reviewed;         406 AA.
AC   Q9FLC2; O81817; Q8LFE4;
DT   30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 134.
DE   RecName: Full=Monooxygenase 3 {ECO:0000303|PubMed:10216258};
DE            Short=AtMO3 {ECO:0000303|PubMed:10216258};
DE            EC=1.14.13.-;
GN   Name=MO3 {ECO:0000303|PubMed:10216258};
GN   OrderedLocusNames=At5g05320 {ECO:0000312|Araport:AT5G05320};
GN   ORFNames=K18I23.12 {ECO:0000312|EMBL:BAB09975.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA   Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT   features of the regions of 1,381,565 bp covered by twenty one physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:131-145(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 156-227, TISSUE SPECIFICITY, GENE FAMILY, AND
RP   NOMENCLATURE.
RC   STRAIN=cv. Columbia;
RX   PubMed=10216258; DOI=10.1016/s0378-1119(99)00073-6;
RA   Aubourg S., Picaud A., Kreis M., Lecharny A.;
RT   "Structure and expression of three src2 homologues and a novel subfamily of
RT   flavoprotein monooxygenase genes revealed by the analysis of a 25kb
RT   fragment from Arabidopsis thaliana chromosome IV.";
RL   Gene 230:197-205(1999).
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000250|UniProtKB:Q5EXK1};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q5EXK1}.
CC   -!- TISSUE SPECIFICITY: Expressed in seeds, seedlings, roots, leaves,
CC       flowers, pollen and siliques. {ECO:0000269|PubMed:10216258}.
CC   -!- SIMILARITY: Belongs to the 3-hydroxybenzoate 6-hydroxylase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM61460.1; Type=Frameshift; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AB010692; BAB09975.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED90857.1; -; Genomic_DNA.
DR   EMBL; BT003148; AAO24580.1; -; mRNA.
DR   EMBL; AK227657; BAE99644.1; -; mRNA.
DR   EMBL; AY084897; AAM61460.1; ALT_FRAME; mRNA.
DR   EMBL; AJ007589; CAA07576.1; -; mRNA.
DR   PIR; T51605; T51605.
DR   RefSeq; NP_196151.1; NM_120614.3.
DR   AlphaFoldDB; Q9FLC2; -.
DR   SMR; Q9FLC2; -.
DR   STRING; 3702.AT5G05320.1; -.
DR   PaxDb; Q9FLC2; -.
DR   PRIDE; Q9FLC2; -.
DR   ProteomicsDB; 238259; -.
DR   EnsemblPlants; AT5G05320.1; AT5G05320.1; AT5G05320.
DR   GeneID; 830414; -.
DR   Gramene; AT5G05320.1; AT5G05320.1; AT5G05320.
DR   KEGG; ath:AT5G05320; -.
DR   Araport; AT5G05320; -.
DR   TAIR; locus:2153499; AT5G05320.
DR   eggNOG; KOG2614; Eukaryota.
DR   HOGENOM; CLU_009665_10_2_1; -.
DR   InParanoid; Q9FLC2; -.
DR   OMA; MEDCYHL; -.
DR   OrthoDB; 521070at2759; -.
DR   PhylomeDB; Q9FLC2; -.
DR   PRO; PR:Q9FLC2; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FLC2; baseline and differential.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.50.50.60; -; 1.
DR   InterPro; IPR002938; FAD-bd.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR044560; MO/PqsH.
DR   PANTHER; PTHR45934; PTHR45934; 1.
DR   Pfam; PF01494; FAD_binding_3; 1.
DR   SUPFAM; SSF51905; SSF51905; 1.
PE   2: Evidence at transcript level;
KW   Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..406
FT                   /note="Monooxygenase 3"
FT                   /id="PRO_0000441116"
FT   CONFLICT        104
FT                   /note="V -> I (in Ref. 5; AAM61460)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        227
FT                   /note="L -> I (in Ref. 6; CAA07576)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   406 AA;  45607 MW;  3DF05BA2E8B914EB CRC64;
     MEAESTQDII IVGAGISGLA TALGLHRLGI RSIVLESSEQ LRATGFALSL YFNAWKAMEA
     LGISQHIRSL GDRFQGWVVR PISAGDPPKE MLFPESEEYE VRCVQRKLLL DALAGELPQG
     TIRFSSKLVH IELSGHYKMV HLSDGTILKT KVLVGCDGVK SVVGKWLGFK NPVKTSRVAI
     RGIAHFQTGH ELGRRFFQFY GNGVRSGFIS CDQNTVYWFL THTSTDLDKK NHQKIKQFVL
     TKIKDLPDNI KSILETTDLD SLVMNPLMYR PPWELLWANI AKDNVCVAGD ALHPMTPDIG
     QGGCSAMEDG VILARCLGEA MKAKNMKGET EDENESYRRI EDGLKKYAGS RKWRSIDLIT
     TSYTVGFIQQ SRGKWMTLFR DKFMSSFLSW LRVKKSHFNC GRLSHE