MOA1_SCHPO
ID MOA1_SCHPO Reviewed; 172 AA.
AC Q9UTI4;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Monopolar attachment protein 1;
DE AltName: Full=Meiotically up-regulated gene 159 protein;
GN Name=moa1; Synonyms=mug159; ORFNames=SPAC15E1.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP DEVELOPMENTAL STAGE, AND INTERACTION WITH REC8.
RX PubMed=16325576; DOI=10.1016/j.cell.2005.09.013;
RA Yokobayashi S., Watanabe Y.;
RT "The kinetochore protein moa1 enables cohesion-mediated monopolar
RT attachment at meiosis I.";
RL Cell 123:803-817(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP FUNCTION IN MEIOSIS.
RX PubMed=16303567; DOI=10.1016/j.cub.2005.10.038;
RA Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L.,
RA Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P.,
RA Smith G.R., Moreno S.;
RT "A large-scale screen in S. pombe identifies seven novel genes required for
RT critical meiotic events.";
RL Curr. Biol. 15:2056-2062(2005).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
RN [5]
RP FUNCTION, INTERACTION WITH PLO1, SUBCELLULAR LOCATION, AND MUTAGENESIS OF
RP THR-101.
RX PubMed=25533956; DOI=10.1038/nature14097;
RA Kim J., Ishiguro K., Nambu A., Akiyoshi B., Yokobayashi S., Kagami A.,
RA Ishiguro T., Pendas A.M., Takeda N., Sakakibara Y., Kitajima T.S.,
RA Tanno Y., Sakuno T., Watanabe Y.;
RT "Meikin is a conserved regulator of meiosis-I-specific kinetochore
RT function.";
RL Nature 517:466-471(2015).
CC -!- FUNCTION: Plays an important role in chromosome segregation during
CC meiosis I by allowing meiotic rec8 to establish cohesion at the
CC centromeric central core and thereby promote the side-by-side structure
CC of kinetochores at meiosis I. Enables monopolar attachment during
CC meiosis I (PubMed:16303567, PubMed:16325576). Required to facilitate
CC kinetochore mono-orientation during meiosis I, when kinetochores on
CC sister chromosomes face the same direction and are thus captured and
CC pulled by spindle fibers from the same pole (PubMed:25533956). Acts in
CC collaboration with plo1 (PubMed:25533956).
CC {ECO:0000269|PubMed:16303567, ECO:0000269|PubMed:16325576,
CC ECO:0000269|PubMed:25533956}.
CC -!- SUBUNIT: Interacts with rec8 (PubMed:16325576), Interacts with plo1
CC (PubMed:25533956). {ECO:0000269|PubMed:16325576,
CC ECO:0000269|PubMed:25533956}.
CC -!- INTERACTION:
CC Q9UTI4; P36626: rec8; NbExp=2; IntAct=EBI-1564258, EBI-1564281;
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere
CC {ECO:0000269|PubMed:16325576}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:16325576, ECO:0000305|PubMed:25533956}.
CC Note=Localizes to the centromere. Does not require rec8 for centromeric
CC localization but instead cnp3. {ECO:0000269|PubMed:16325576}.
CC -!- DEVELOPMENTAL STAGE: Present during meiotic prophase I, when monopolar
CC attachment of kinetochores to spindle microtubules is established.
CC First appears during prophase I and localizes to several punctate dots
CC in the nucleus until metaphase I. At the onset of anaphase I, it
CC decreases markedly and remains absent until telophase I. Does not
CC reappear during meiosis II. {ECO:0000269|PubMed:16325576}.
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DR EMBL; AB232928; BAE46564.1; -; Genomic_DNA.
DR EMBL; CU329670; CAB52426.1; -; Genomic_DNA.
DR PIR; T37722; T37722.
DR RefSeq; NP_594308.1; NM_001019731.1.
DR AlphaFoldDB; Q9UTI4; -.
DR BioGRID; 279256; 11.
DR IntAct; Q9UTI4; 3.
DR STRING; 4896.SPAC15E1.07c.1; -.
DR PaxDb; Q9UTI4; -.
DR EnsemblFungi; SPAC15E1.07c.1; SPAC15E1.07c.1:pep; SPAC15E1.07c.
DR GeneID; 2542808; -.
DR KEGG; spo:SPAC15E1.07c; -.
DR PomBase; SPAC15E1.07c; moa1.
DR VEuPathDB; FungiDB:SPAC15E1.07c; -.
DR HOGENOM; CLU_1556158_0_0_1; -.
DR PRO; PR:Q9UTI4; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0061638; C:CENP-A containing chromatin; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IC:PomBase.
DR GO; GO:0140483; F:kinetochore adaptor activity; EXP:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:PomBase.
DR GO; GO:1990813; P:meiotic centromeric cohesion protection; IMP:PomBase.
DR GO; GO:0045132; P:meiotic chromosome segregation; IMP:PomBase.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IMP:PomBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Kinetochore; Meiosis; Nucleus; Reference proteome.
FT CHAIN 1..172
FT /note="Monopolar attachment protein 1"
FT /id="PRO_0000076264"
FT REGION 15..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 100..102
FT /note="POLO box domain (PBD)-binding"
FT /evidence="ECO:0000305|PubMed:25533956"
FT COMPBIAS 26..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 101
FT /note="T->A: Abolition of meiosis-specific enrichment of
FT plo1 to kinetochores."
FT /evidence="ECO:0000269|PubMed:25533956"
SQ SEQUENCE 172 AA; 19612 MW; BAC48C2E18157031 CRC64;
MAINNENELE YKLIKKNKNP KISNSKKKNS TRPALQDKTN QTLPIHQNQA FSNILPSDFS
IIKTPETKTA DDFPVNGYEG LNILKFDLEL FYKLKPVATS TPKSCMRTGS NLFLNETVKH
VPDERLVSNI KNTQTKDSIT RDSAYYHRKT MTESIIKTLA AFDAEVDEII LF
