ID   MOAR1_MYCBP             Reviewed;         311 AA.
AC   A1KNB8;
DT   22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT   22-FEB-2012, sequence version 2.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Transcriptional regulatory protein MoaR1;
DE   AltName: Full=Molybdopterin biosynthesis positive regulator;
GN   Name=moaR1; OrderedLocusNames=BCG_3145;
OS   Mycobacterium bovis (strain BCG / Pasteur 1173P2).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=410289;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BCG / Pasteur 1173P2;
RX   PubMed=17372194; DOI=10.1073/pnas.0700869104;
RA   Brosch R., Gordon S.V., Garnier T., Eiglmeier K., Frigui W., Valenti P.,
RA   Dos Santos S., Duthoy S., Lacroix C., Garcia-Pelayo C., Inwald J.K.,
RA   Golby P., Garcia J.N., Hewinson R.G., Behr M.A., Quail M.A., Churcher C.,
RA   Barrell B.G., Parkhill J., Cole S.T.;
RT   "Genome plasticity of BCG and impact on vaccine efficacy.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:5596-5601(2007).
RN   [2]
RP   FUNCTION AS A TRANSCRIPTIONAL REGULATOR.
RC   STRAIN=BCG / Pasteur 1173P2;
RX   PubMed=20378651; DOI=10.1099/mic.0.037200-0;
RA   Mendoza Lopez P., Golby P., Wooff E., Nunez Garcia J., Garcia Pelayo M.C.,
RA   Conlon K., Gema Camacho A., Hewinson R.G., Polaina J., Suarez Garcia A.,
RA   Gordon S.V.;
RT   "Characterization of the transcriptional regulator Rv3124 of Mycobacterium
RT   tuberculosis identifies it as a positive regulator of molybdopterin
RT   biosynthesis and defines the functional consequences of a non-synonymous
RT   SNP in the Mycobacterium bovis BCG orthologue.";
RL   Microbiology 156:2112-2123(2010).
CC   -!- FUNCTION: Acts as a positive transcriptional regulator of the
CC       molybdopterin biosynthesis moa1 locus, promoting the expression of the
CC       moaA1B1C1D1 genes. {ECO:0000269|PubMed:20378651}.
CC   -!- MISCELLANEOUS: Identification of single-nucleotide polymorphisms (SNPs)
CC       between the attenuated M.bovis BCG Pasteur strain and the virulent
CC       M.bovis strain AF2122/97 revealed a E159G mutation in BCG_3145. The
CC       E159G SNP was found to be present in all BCG strains, but absent from
CC       virulent M.bovis and Mycobacterium tuberculosis strains. The SNP in
CC       BCG_3145 was found to have a subtle effect on the activity of MoaR1,
CC       suggesting that this mutation is not a key event in the virulence
CC       attenuation of BCG.
CC   -!- SIMILARITY: Belongs to the AfsR/DnrI/RedD regulatory family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAL73134.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AM408590; CAL73134.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; A1KNB8; -.
DR   SMR; A1KNB8; -.
DR   KEGG; mbb:BCG_3145; -.
DR   HOGENOM; CLU_004665_0_0_11; -.
DR   Proteomes; UP000001472; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd15831; BTAD; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.25.40.10; -; 1.
DR   InterPro; IPR005158; BTAD.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF03704; BTAD; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM01043; BTAD; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF48452; SSF48452; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Transcription; Transcription regulation.
FT   CHAIN           1..311
FT                   /note="Transcriptional regulatory protein MoaR1"
FT                   /id="PRO_0000415514"
FT   DNA_BIND        15..117
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
SQ   SEQUENCE   311 AA;  34453 MW;  3C735BC6CEDE5049 CRC64;
     MGQRPFSPNH RSGVLNATTA GAVQFNVLGP LELNLRGTKL PLGTPKQRAV LAMLLLSRNQ
     VVAADALVQA IWEKSPPARA RRTVHTYICN LRRTLSDAGV DSRNILVSEP PGYRLLIGDR
     QQCDLDRFVA AKESGLRASA KGYFSEAIRY LDSALQNWRG PVLGDLRSFM FVQMFSRALT
     GDELLVHTKL AEAAIACGRA DVVIPKLERL VAMHPYRESL WKQLMLGYYV NEYQSAAIDA
     YHRLKSTLAE ELGVEPAPTI RALYHKILRQ LPMDDLVGRV TRGRVDLRGG NGAKVEELTE
     SDKDLLPIGL A