MOAR1_MYCTU
ID MOAR1_MYCTU Reviewed; 311 AA.
AC O05797; L0TEE0;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 22-FEB-2012, sequence version 4.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Transcriptional regulatory protein MoaR1;
DE AltName: Full=Molybdopterin biosynthesis positive regulator;
GN Name=moaR1; OrderedLocusNames=Rv3124;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP INDUCTION.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=15207493; DOI=10.1016/j.tube.2003.12.006;
RA Muttucumaru D.G., Roberts G., Hinds J., Stabler R.A., Parish T.;
RT "Gene expression profile of Mycobacterium tuberculosis in a non-replicating
RT state.";
RL Tuberculosis 84:239-246(2004).
RN [3]
RP FUNCTION AS A TRANSCRIPTIONAL REGULATOR, GENE NAME, DNA-BINDING, AND
RP MUTAGENESIS OF GLY-112; TYR-113 AND GLU-181.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=20378651; DOI=10.1099/mic.0.037200-0;
RA Mendoza Lopez P., Golby P., Wooff E., Nunez Garcia J., Garcia Pelayo M.C.,
RA Conlon K., Gema Camacho A., Hewinson R.G., Polaina J., Suarez Garcia A.,
RA Gordon S.V.;
RT "Characterization of the transcriptional regulator Rv3124 of Mycobacterium
RT tuberculosis identifies it as a positive regulator of molybdopterin
RT biosynthesis and defines the functional consequences of a non-synonymous
RT SNP in the Mycobacterium bovis BCG orthologue.";
RL Microbiology 156:2112-2123(2010).
CC -!- FUNCTION: Acts as a positive transcriptional regulator of the
CC molybdopterin biosynthesis moa1 locus, promoting the expression of the
CC moaA1B1C1D1 genes. Binds directly to the moaA1 promoter.
CC {ECO:0000269|PubMed:20378651}.
CC -!- INDUCTION: Expression of MoaR1 appears to be modulated by oxygen
CC availability since it is up-regulated in both microaerophilic (NRP1)
CC and anaerobic (NRP2) cultures compared with aerobic cultures; thus,
CC seems to be up-regulated during the shift into the persistent state in
CC the human host. {ECO:0000269|PubMed:15207493}.
CC -!- SIMILARITY: Belongs to the AfsR/DnrI/RedD regulatory family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCP45934.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AL123456; CCP45934.1; ALT_INIT; Genomic_DNA.
DR PIR; C70922; C70922.
DR RefSeq; NP_217640.1; NC_000962.3.
DR AlphaFoldDB; O05797; -.
DR SMR; O05797; -.
DR STRING; 83332.Rv3124; -.
DR PaxDb; O05797; -.
DR DNASU; 888825; -.
DR GeneID; 888825; -.
DR KEGG; mtu:Rv3124; -.
DR TubercuList; Rv3124; -.
DR eggNOG; COG3629; Bacteria.
DR InParanoid; O05797; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0003677; F:DNA binding; IDA:MTBBASE.
DR GO; GO:0032324; P:molybdopterin cofactor biosynthetic process; IEP:MTBBASE.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEP:MTBBASE.
DR CDD; cd15831; BTAD; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR005158; BTAD.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03704; BTAD; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM01043; BTAD; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..311
FT /note="Transcriptional regulatory protein MoaR1"
FT /id="PRO_0000415513"
FT DNA_BIND 15..117
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MUTAGEN 112
FT /note="G->A: Has no deleterious effect on the transcription
FT of the moa1 locus."
FT /evidence="ECO:0000269|PubMed:20378651"
FT MUTAGEN 113
FT /note="Y->A: Shows significantly reduced transcription of
FT the moa1 locus."
FT /evidence="ECO:0000269|PubMed:20378651"
FT MUTAGEN 181
FT /note="E->D: Has no deleterious effect on the transcription
FT of the moa1 locus."
FT /evidence="ECO:0000269|PubMed:20378651"
FT MUTAGEN 181
FT /note="E->I,W: Completely disrupts protein function, as
FT shown by the dramatic decrease of RNA levels for the moa1
FT locus."
FT /evidence="ECO:0000269|PubMed:20378651"
FT MUTAGEN 181
FT /note="E->Q: Shows reduced transcription of the moa1
FT locus."
FT /evidence="ECO:0000269|PubMed:20378651"
SQ SEQUENCE 311 AA; 34525 MW; 3EF95A2CCDBE5049 CRC64;
MGQRPFSPNH RSGVLNATTA GAVQFNVLGP LELNLRGTKL PLGTPKQRAV LAMLLLSRNQ
VVAADALVQA IWEKSPPARA RRTVHTYICN LRRTLSDAGV DSRNILVSEP PGYRLLIGDR
QQCDLDRFVA AKESGLRASA KGYFSEAIRY LDSALQNWRG PVLGDLRSFM FVQMFSRALT
EDELLVHTKL AEAAIACGRA DVVIPKLERL VAMHPYRESL WKQLMLGYYV NEYQSAAIDA
YHRLKSTLAE ELGVEPAPTI RALYHKILRQ LPMDDLVGRV TRGRVDLRGG NGAKVEELTE
SDKDLLPIGL A
