MOB2_DROME
ID MOB2_DROME Reviewed; 566 AA.
AC Q8IQG1; Q5BHY7; Q8IGB4; Q8IQG2; Q9VTJ6; Q9VTJ7;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 3.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=MOB kinase activator-like 2;
DE AltName: Full=Mob as tumor suppressor protein 2;
DE Short=Dmob2;
DE AltName: Full=Mps one binder kinase activator-like 2;
GN Name=Mob2; ORFNames=CG11711;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAN11874.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAN11874.2}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAN71631.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM D).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAN71631.1};
RC TISSUE=Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAX33585.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAX33585.1}; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, INTERACTION WITH TRC, AND SUBCELLULAR LOCATION.
RX PubMed=15975907; DOI=10.1091/mbc.e05-01-0018;
RA He Y., Emoto K., Fang X., Ren N., Tian X., Jan Y.-N., Adler P.N.;
RT "Drosophila Mob family proteins interact with the related tricornered (Trc)
RT and warts (Wts) kinases.";
RL Mol. Biol. Cell 16:4139-4152(2005).
RN [6] {ECO:0000305}
RP RNA EDITING OF POSITION 91.
RX PubMed=17018572; DOI=10.1261/rna.254306;
RA Stapleton M., Carlson J.W., Celniker S.E.;
RT "RNA editing in Drosophila melanogaster: new targets and functional
RT consequences.";
RL RNA 12:1922-1932(2006).
CC -!- FUNCTION: Required for the normal morphogenesis of a variety of
CC polarized outgrowths including epidermal hairs, bristles, arista
CC laterals, and dendrites. {ECO:0000269|PubMed:15975907}.
CC -!- SUBUNIT: Interacts with and activates trc, also interacts with wts.
CC {ECO:0000269|PubMed:15975907}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15975907}. Nucleus
CC {ECO:0000269|PubMed:15975907}. Note=Trc colocalizes with Mob1 to the
CC cell periphery in wing cells and wing hairs.
CC {ECO:0000269|PubMed:15975907}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=D {ECO:0000269|PubMed:10731132};
CC IsoId=Q8IQG1-1; Sequence=Displayed;
CC Name=A {ECO:0000269|PubMed:10731132};
CC IsoId=Q8IQG1-2; Sequence=VSP_052339;
CC Name=B {ECO:0000269|PubMed:10731132};
CC IsoId=Q8IQG1-3; Sequence=VSP_052340;
CC Name=C {ECO:0000269|PubMed:10731132};
CC IsoId=Q8IQG1-4; Sequence=VSP_052341;
CC -!- RNA EDITING: Modified_positions=91 {ECO:0000269|PubMed:17018572};
CC Note=Partially edited. Target of Adar. {ECO:0000269|PubMed:17018572};
CC -!- SIMILARITY: Belongs to the MOB1/phocein family. {ECO:0000255}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN71631.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAX33585.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF50051.1; -; Genomic_DNA.
DR EMBL; AE014296; AAF50052.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN11873.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN11874.2; -; Genomic_DNA.
DR EMBL; BT001864; AAN71631.1; ALT_FRAME; mRNA.
DR EMBL; BT021437; AAX33585.1; ALT_SEQ; mRNA.
DR RefSeq; NP_001261713.1; NM_001274784.1. [Q8IQG1-3]
DR RefSeq; NP_648474.1; NM_140217.2. [Q8IQG1-4]
DR RefSeq; NP_729714.1; NM_168460.2. [Q8IQG1-1]
DR RefSeq; NP_729715.2; NM_168461.2. [Q8IQG1-2]
DR RefSeq; NP_729716.1; NM_168462.2. [Q8IQG1-3]
DR AlphaFoldDB; Q8IQG1; -.
DR SMR; Q8IQG1; -.
DR BioGRID; 64660; 37.
DR IntAct; Q8IQG1; 9.
DR STRING; 7227.FBpp0088371; -.
DR PaxDb; Q8IQG1; -.
DR DNASU; 39293; -.
DR EnsemblMetazoa; FBtr0089330; FBpp0088369; FBgn0259481. [Q8IQG1-4]
DR EnsemblMetazoa; FBtr0089331; FBpp0088370; FBgn0259481. [Q8IQG1-1]
DR EnsemblMetazoa; FBtr0089332; FBpp0088371; FBgn0259481. [Q8IQG1-2]
DR EnsemblMetazoa; FBtr0089333; FBpp0088372; FBgn0259481. [Q8IQG1-3]
DR EnsemblMetazoa; FBtr0333217; FBpp0305419; FBgn0259481. [Q8IQG1-3]
DR GeneID; 39293; -.
DR KEGG; dme:Dmel_CG11711; -.
DR UCSC; CG11711-RA; d. melanogaster. [Q8IQG1-1]
DR CTD; 81532; -.
DR FlyBase; FBgn0259481; Mob2.
DR VEuPathDB; VectorBase:FBgn0259481; -.
DR eggNOG; KOG0440; Eukaryota.
DR InParanoid; Q8IQG1; -.
DR OMA; MYLWFDE; -.
DR SignaLink; Q8IQG1; -.
DR BioGRID-ORCS; 39293; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Mob2; fly.
DR GenomeRNAi; 39293; -.
DR PRO; PR:Q8IQG1; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0259481; Expressed in brain and 20 other tissues.
DR ExpressionAtlas; Q8IQG1; baseline and differential.
DR GO; GO:0070451; C:cell hair; IDA:FlyBase.
DR GO; GO:0071944; C:cell periphery; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR GO; GO:0016028; C:rhabdomere; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0030295; F:protein kinase activator activity; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IPI:FlyBase.
DR GO; GO:0000902; P:cell morphogenesis; IMP:UniProtKB.
DR GO; GO:0007616; P:long-term memory; IMP:FlyBase.
DR GO; GO:0045886; P:negative regulation of synaptic assembly at neuromuscular junction; IMP:FlyBase.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IBA:GO_Central.
DR GO; GO:0042052; P:rhabdomere development; IMP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR Gene3D; 1.20.140.30; -; 1.
DR InterPro; IPR005301; MOB_kinase_act_fam.
DR InterPro; IPR036703; MOB_kinase_act_sf.
DR PANTHER; PTHR22599; PTHR22599; 1.
DR Pfam; PF03637; Mob1_phocein; 1.
DR SMART; SM01388; Mob1_phocein; 1.
DR SUPFAM; SSF101152; SSF101152; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Metal-binding; Nucleus;
KW Reference proteome; RNA editing; Zinc.
FT CHAIN 1..566
FT /note="MOB kinase activator-like 2"
FT /id="PRO_0000279701"
FT REGION 28..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 74..118
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 346..407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 498..541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 507..527
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 209
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9H8S9"
FT BINDING 214
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9H8S9"
FT BINDING 289
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9H8S9"
FT BINDING 294
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:Q9H8S9"
FT VAR_SEQ 2..137
FT /note="NWAISNLTGGNRVSRAILVRYSSVPLADATNDGSSTAPQTPTASTPRPSSSH
FT SSLSSTFAATFLHASSRHIPGRAVPRQNANGQNGGKGNASGAGGGAGGGGAGGASGGTG
FT GTGAQAAGQLSLPLDVEETVTCFCR -> LRAPTQHIVQIGFGTKTTTTTTTTAAAAAA
FT TSTAAAATPGGKQQQQRKTLLQRFRQQQHKLPKVAAAEQTTHDTEYYFKQQQQQQTSSY
FT QQGDNCLLYLATTLNSNSEQLLKKSKKKKESSSYNFAAALRRNKKPKQQQQQLATSTDD
FT DDSCRSINSINSNCSNSSTSGSSHSLSNSCINNHNNTHYRNSKNTRHSSSNNNDYSGNY
FT NCKTSEQQAWKASAAQQLLISDRNYPSSNTNNTNNNTNNITNSSNNNHSGGHIIMSPTS
FT QAPTRTSLFDSCHRKIRLIGGGPVAFLGGLVA (in isoform A)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_052339"
FT VAR_SEQ 2..137
FT /note="NWAISNLTGGNRVSRAILVRYSSVPLADATNDGSSTAPQTPTASTPRPSSSH
FT SSLSSTFAATFLHASSRHIPGRAVPRQNANGQNGGKGNASGAGGGAGGGGAGGASGGTG
FT GTGAQAAGQLSLPLDVEETVTCFCR -> KETLSSKVPTRIGVTFVSESDPKKKFKSSP
FT STSSSTISTATITSTTTTTTTTASPPPPQTSYVIKCLLKTARFMWQVTTIPSKIGDTIG
FT TLYRYAQDSVDSFLCVAG (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_052340"
FT VAR_SEQ 2..137
FT /note="NWAISNLTGGNRVSRAILVRYSSVPLADATNDGSSTAPQTPTASTPRPSSSH
FT SSLSSTFAATFLHASSRHIPGRAVPRQNANGQNGGKGNASGAGGGAGGGGAGGASGGTG
FT GTGAQAAGQLSLPLDVEETVTCFCR -> G (in isoform C)"
FT /evidence="ECO:0000303|PubMed:10731132, ECO:0000303|Ref.4"
FT /id="VSP_052341"
FT VARIANT 91
FT /note="N -> D (in RNA edited version)"
FT /evidence="ECO:0000269|PubMed:17018572"
SQ SEQUENCE 566 AA; 58907 MW; 1D3F57A2659DEC2C CRC64;
MNWAISNLTG GNRVSRAILV RYSSVPLADA TNDGSSTAPQ TPTASTPRPS SSHSSLSSTF
AATFLHASSR HIPGRAVPRQ NANGQNGGKG NASGAGGGAG GGGAGGASGG TGGTGAQAAG
QLSLPLDVEE TVTCFCRKAR RKERDGDQNS TDTKLYLEES VLERKLPEAD LKALVDLPAG
LDYNEWLASH TLALFEHVNL VYGTISEFCT QSGCADMTGP GNRTYLWFDE KGKKTRVAAP
QYIDYVMTFT QKTVSDESIF PTKYANEFPG SFESIARKIL RLQFHVIAHL YAAHFREIAL
LGLHTHLNLT FAHLTALHRR FNLIDEKETD VLRDLEVALR LTDDTGGCQD ATSTTSSVHD
HSHSGDLQHQ SLQQQQQHHN SSSNSTSSAE AFHVNSQSNN GSTSASASVS LIDGDAVAPP
ICTQPEAGAG CKPAGSSGLL GGILGDLTSG EFGDTTRYCT SAVPQAAAAA GAGVGGTAIG
ATDAAALNNG AGALHLNFSN NNNNNHNLNH HHHHHHHHGH HGHHHAAQQQ QQHSGLIQCN
AAGGGGNATG VATGGATAAA SSTTTA
