MOC2A_EMENI
ID MOC2A_EMENI Reviewed; 91 AA.
AC Q9Y8C2; C8VK56; Q5ARD7;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Molybdopterin synthase sulfur carrier subunit {ECO:0000255|HAMAP-Rule:MF_03051};
DE AltName: Full=Common component for nitrate reductase and xanthine dehydrogenase protein G {ECO:0000255|HAMAP-Rule:MF_03051};
DE AltName: Full=Molybdenum cofactor synthesis protein 2 small subunit {ECO:0000255|HAMAP-Rule:MF_03051};
DE AltName: Full=Molybdenum cofactor synthesis protein 2A {ECO:0000255|HAMAP-Rule:MF_03051};
DE Short=MOCS2A {ECO:0000255|HAMAP-Rule:MF_03051};
DE AltName: Full=Sulfur carrier protein MOCS2A {ECO:0000255|HAMAP-Rule:MF_03051};
GN Name=cnxG {ECO:0000255|HAMAP-Rule:MF_03051}; ORFNames=AN9143;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF TYR-8; ALA-10;
RP ALA-12; GLU-21; ILE-84 AND GLY-91.
RX PubMed=10383438; DOI=10.1074/jbc.274.27.19286;
RA Unkles S.E., Heck I.S., Appleyard M.V.C.L., Kinghorn J.R.;
RT "Eukaryotic molybdopterin synthase. Biochemical and molecular studies of
RT Aspergillus nidulans cnxG and cnxH mutants.";
RL J. Biol. Chem. 274:19286-19293(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
CC -!- FUNCTION: Acts as a sulfur carrier required for molybdopterin
CC biosynthesis. Component of the molybdopterin synthase complex that
CC catalyzes the conversion of precursor Z into molybdopterin by mediating
CC the incorporation of 2 sulfur atoms into precursor Z to generate a
CC dithiolene group. In the complex, serves as sulfur donor by being
CC thiocarboxylated (-COSH) at its C-terminus by uba4. After interaction
CC with MOCS2B, the sulfur is then transferred to precursor Z to form
CC molybdopterin. {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- SUBUNIT: Heterotetramer; composed of 2 small (MOCS2A) and 2 large
CC (MOCS2B) subunits. {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-
CC adenylated (-COAMP) via the hesA/moeB/thiF part of uba4, then
CC thiocarboxylated (-COSH) via the rhodanese domain of uba4.
CC {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- SIMILARITY: Belongs to the MoaD family. MOCS2A subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_03051}.
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DR EMBL; AF138284; AAD39470.1; -; Genomic_DNA.
DR EMBL; AACD01000169; EAA61976.1; -; Genomic_DNA.
DR EMBL; BN001306; CBF82462.1; -; Genomic_DNA.
DR RefSeq; XP_682412.1; XM_677320.1.
DR AlphaFoldDB; Q9Y8C2; -.
DR SMR; Q9Y8C2; -.
DR STRING; 162425.CADANIAP00009465; -.
DR EnsemblFungi; CBF82462; CBF82462; ANIA_09143.
DR EnsemblFungi; EAA61976; EAA61976; AN9143.2.
DR GeneID; 2868024; -.
DR KEGG; ani:AN9143.2; -.
DR VEuPathDB; FungiDB:AN9143; -.
DR eggNOG; ENOG502SD3I; Eukaryota.
DR HOGENOM; CLU_114601_6_1_1; -.
DR OMA; KTRMIEC; -.
DR OrthoDB; 1631547at2759; -.
DR BRENDA; 2.8.1.12; 517.
DR UniPathway; UPA00344; -.
DR Proteomes; UP000000560; Chromosome VI.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:0019008; C:molybdopterin synthase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0030366; F:molybdopterin synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0032324; P:molybdopterin cofactor biosynthetic process; IMP:AspGD.
DR Gene3D; 3.10.20.30; -; 1.
DR HAMAP; MF_03051; MOCS2A; 1.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR044672; MOCS2A.
DR InterPro; IPR028887; MOCS2A_euk.
DR InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
DR InterPro; IPR003749; ThiS/MoaD-like.
DR PANTHER; PTHR33359; PTHR33359; 1.
DR Pfam; PF02597; ThiS; 1.
DR SUPFAM; SSF54285; SSF54285; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Molybdenum cofactor biosynthesis; Nucleotide-binding;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..91
FT /note="Molybdopterin synthase sulfur carrier subunit"
FT /id="PRO_0000369322"
FT MOD_RES 91
FT /note="1-thioglycine; alternate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03051"
FT MOD_RES 91
FT /note="Glycyl adenylate; alternate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03051"
FT MUTAGEN 8
FT /note="Y->C: In cnxG141; impairs molybdopterin
FT biosynthesis."
FT /evidence="ECO:0000269|PubMed:10383438"
FT MUTAGEN 10
FT /note="A->D: In cnxG24; impairs molybdopterin
FT biosynthesis."
FT /evidence="ECO:0000269|PubMed:10383438"
FT MUTAGEN 12
FT /note="A->V: In cnxG20; impairs molybdopterin
FT biosynthesis."
FT /evidence="ECO:0000269|PubMed:10383438"
FT MUTAGEN 21
FT /note="E->K: In cnxG100; impairs molybdopterin
FT biosynthesis."
FT /evidence="ECO:0000269|PubMed:10383438"
FT MUTAGEN 84
FT /note="I->N: In cnxG2; impairs molybdopterin biosynthesis."
FT /evidence="ECO:0000269|PubMed:10383438"
FT MUTAGEN 91
FT /note="G->R: In cnxG4; impairs molybdopterin biosynthesis."
FT /evidence="ECO:0000269|PubMed:10383438"
SQ SEQUENCE 91 AA; 9599 MW; CB03DE857DEFCE70 CRC64;
MSTFQIHYFA SASTYTGRNT ESLPAPLPLS SLFDTLEAKY PGIKEKVLSS CSISLGDEYV
DLVSDGEKSG NEGLLIQGGD EVAIIPPVSS G
