MOC2A_MAIZE
ID MOC2A_MAIZE Reviewed; 93 AA.
AC B6SXF8; Q2Q066;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Molybdopterin synthase sulfur carrier subunit {ECO:0000255|HAMAP-Rule:MF_03051};
DE AltName: Full=Molybdenum cofactor synthesis protein 2 small subunit {ECO:0000255|HAMAP-Rule:MF_03051};
DE AltName: Full=Molybdenum cofactor synthesis protein 2A {ECO:0000255|HAMAP-Rule:MF_03051};
DE Short=MOCS2A {ECO:0000255|HAMAP-Rule:MF_03051};
DE AltName: Full=Protein viviparous15;
DE AltName: Full=Sulfur carrier protein MOCS2A {ECO:0000255|HAMAP-Rule:MF_03051};
GN Name=VP15;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=16367969; DOI=10.1111/j.1365-313x.2005.02620.x;
RA Suzuki M., Settles A.M., Tseung C.-W., Li Q.-B., Latshaw S., Wu S.,
RA Porch T.G.P., Schmelz E.A., James M.G., McCarty D.R.;
RT "The maize viviparous15 locus encodes the molybdopterin synthase small
RT subunit.";
RL Plant J. 45:264-274(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
CC -!- FUNCTION: Acts as a sulfur carrier required for molybdopterin
CC biosynthesis. Component of the molybdopterin synthase complex that
CC catalyzes the conversion of precursor Z into molybdopterin by mediating
CC the incorporation of 2 sulfur atoms into precursor Z to generate a
CC dithiolene group. In the complex, serves as sulfur donor by being
CC thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction
CC with MOCS2B, the sulfur is then transferred to precursor Z to form
CC molybdopterin. {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- SUBUNIT: Heterotetramer; composed of 2 small (MOCS2A) and 2 large
CC (MOCS2B) subunits. {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- TISSUE SPECIFICITY: Expressed in both embryo and endosperm during seed
CC maturation. {ECO:0000269|PubMed:16367969}.
CC -!- PTM: C-terminal thiocarboxylation occurs in 2 steps, it is first acyl-
CC adenylated (-COAMP) via the hesA/moeB/thiF part of MOCS3, then
CC thiocarboxylated (-COSH) via the rhodanese domain of MOCS3.
CC {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- DISRUPTION PHENOTYPE: Precocious germination and early seedling
CC lethality; probably due to molybdenum cofactor (MoCo) defects, a
CC cofactor required for abscisic acid (ABA) biosynthesis.
CC {ECO:0000269|PubMed:16367969}.
CC -!- SIMILARITY: Belongs to the MoaD family. MOCS2A subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_03051}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABB96257.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ACG29510.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ACG32695.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; DQ273133; ABB96257.1; ALT_INIT; Genomic_DNA.
DR EMBL; EU957392; ACG29510.1; ALT_INIT; mRNA.
DR EMBL; EU957423; ACG29541.1; -; mRNA.
DR EMBL; EU960577; ACG32695.1; ALT_INIT; mRNA.
DR RefSeq; NP_001148029.1; NM_001154557.1.
DR RefSeq; XP_008643630.1; XM_008645408.1.
DR AlphaFoldDB; B6SXF8; -.
DR SMR; B6SXF8; -.
DR STRING; 4577.GRMZM2G121468_P02; -.
DR PaxDb; B6SXF8; -.
DR EnsemblPlants; Zm00001eb244080_T003; Zm00001eb244080_P003; Zm00001eb244080.
DR GeneID; 100281638; -.
DR Gramene; Zm00001eb244080_T003; Zm00001eb244080_P003; Zm00001eb244080.
DR KEGG; zma:100281638; -.
DR eggNOG; KOG3474; Eukaryota.
DR UniPathway; UPA00344; -.
DR Proteomes; UP000007305; Chromosome 5.
DR ExpressionAtlas; B6SXF8; baseline and differential.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:0019008; C:molybdopterin synthase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0030366; F:molybdopterin synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.20.30; -; 1.
DR HAMAP; MF_03051; MOCS2A; 1.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR044672; MOCS2A.
DR InterPro; IPR028887; MOCS2A_euk.
DR InterPro; IPR016155; Mopterin_synth/thiamin_S_b.
DR InterPro; IPR003749; ThiS/MoaD-like.
DR PANTHER; PTHR33359; PTHR33359; 1.
DR Pfam; PF02597; ThiS; 1.
DR SUPFAM; SSF54285; SSF54285; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Molybdenum cofactor biosynthesis; Nucleotide-binding;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..93
FT /note="Molybdopterin synthase sulfur carrier subunit"
FT /id="PRO_0000369317"
FT MOD_RES 93
FT /note="1-thioglycine; alternate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03051"
FT MOD_RES 93
FT /note="Glycyl adenylate; alternate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03051"
SQ SEQUENCE 93 AA; 9393 MW; AC4C48E72150E2B4 CRC64;
MPAAAEEQAA PAATVKVLFF ARARDLTGVA DSAVEVPPGS TAGECLARVL AQFPKLEEIR
GSVVLALNEE YAADSAAVAD GDELAVIPPI SGG
