MOC2B_BOVIN
ID MOC2B_BOVIN Reviewed; 189 AA.
AC A4FUY7; Q58DT2;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 2.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Molybdopterin synthase catalytic subunit {ECO:0000255|HAMAP-Rule:MF_03052};
DE EC=2.8.1.12 {ECO:0000255|HAMAP-Rule:MF_03052};
DE AltName: Full=Molybdenum cofactor synthesis protein 2 large subunit {ECO:0000255|HAMAP-Rule:MF_03052};
DE AltName: Full=Molybdenum cofactor synthesis protein 2B {ECO:0000255|HAMAP-Rule:MF_03052};
DE Short=MOCS2B {ECO:0000255|HAMAP-Rule:MF_03052};
GN Name=MOCS2 {ECO:0000255|HAMAP-Rule:MF_03052};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT "Characterization of 954 bovine full-CDS cDNA sequences.";
RL BMC Genomics 6:166-166(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Hippocampus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalytic subunit of the molybdopterin synthase complex, a
CC complex that catalyzes the conversion of precursor Z into
CC molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms
CC from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene
CC group. {ECO:0000255|HAMAP-Rule:MF_03052}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal
CC Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O = 2
CC [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly +
CC 4 H(+) + molybdopterin; Xref=Rhea:RHEA:26333, Rhea:RHEA-COMP:12160,
CC Rhea:RHEA-COMP:12202, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58698, ChEBI:CHEBI:59648, ChEBI:CHEBI:90619,
CC ChEBI:CHEBI:90778; EC=2.8.1.12; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_03052};
CC -!- PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_03052}.
CC -!- SUBUNIT: Heterotetramer; composed of 2 small (MOCS2A) and 2 large
CC (MOCS2B) subunits. {ECO:0000255|HAMAP-Rule:MF_03052}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000255|HAMAP-
CC Rule:MF_03052}.
CC -!- MISCELLANEOUS: This protein is produced by a bicistronic gene which
CC also produces the small subunit (MOCS2A) from an overlapping reading
CC frame.
CC -!- SIMILARITY: Belongs to the MoaE family. MOCS2B subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_03052}.
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DR EMBL; BT021515; AAX46362.1; -; mRNA.
DR EMBL; BC123458; AAI23459.2; -; mRNA.
DR RefSeq; NP_001014867.1; NM_001014867.1.
DR RefSeq; XP_005221575.1; XM_005221518.3.
DR RefSeq; XP_005221576.1; XM_005221519.3.
DR AlphaFoldDB; A4FUY7; -.
DR SMR; A4FUY7; -.
DR STRING; 9913.ENSBTAP00000007087; -.
DR PaxDb; A4FUY7; -.
DR PRIDE; A4FUY7; -.
DR Ensembl; ENSBTAT00000007087; ENSBTAP00000007087; ENSBTAG00000005380.
DR Ensembl; ENSBTAT00000085411; ENSBTAP00000065304; ENSBTAG00000005380.
DR GeneID; 507986; -.
DR KEGG; bta:507986; -.
DR CTD; 4338; -.
DR VEuPathDB; HostDB:ENSBTAG00000005380; -.
DR VGNC; VGNC:31548; MOCS2.
DR eggNOG; KOG3307; Eukaryota.
DR GeneTree; ENSGT00510000047669; -.
DR HOGENOM; CLU_089568_0_1_1; -.
DR InParanoid; A4FUY7; -.
DR OMA; WPLQRVS; -.
DR OrthoDB; 1419096at2759; -.
DR TreeFam; TF314334; -.
DR UniPathway; UPA00344; -.
DR Proteomes; UP000009136; Chromosome 20.
DR Bgee; ENSBTAG00000005380; Expressed in metanephros cortex and 108 other tissues.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0019008; C:molybdopterin synthase complex; IEA:UniProtKB-UniRule.
DR GO; GO:0016607; C:nuclear speck; IEA:Ensembl.
DR GO; GO:0030366; F:molybdopterin synthase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; ISS:UniProtKB.
DR CDD; cd00756; MoaE; 1.
DR Gene3D; 3.90.1170.40; -; 1.
DR HAMAP; MF_03052; MOC2B; 1.
DR InterPro; IPR036563; MoaE_sf.
DR InterPro; IPR028888; MOCS2B_euk.
DR InterPro; IPR003448; Mopterin_biosynth_MoaE.
DR Pfam; PF02391; MoaE; 1.
DR SUPFAM; SSF54690; SSF54690; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Molybdenum cofactor biosynthesis; Phosphoprotein;
KW Reference proteome; Transferase.
FT CHAIN 1..189
FT /note="Molybdopterin synthase catalytic subunit"
FT /id="PRO_0000369325"
FT BINDING 143..144
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
FT BINDING 159
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
FT BINDING 166..168
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
FT MOD_RES 20
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O96007"
FT CONFLICT 27
FT /note="S -> N (in Ref. 1; AAX46362)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="E -> D (in Ref. 1; AAX46362)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 189 AA; 21173 MW; FA76B20156E07B74 CRC64;
MSSLEISSSC FNQETKLPLS LPLVEGSTFE PPGKDMNEVE EKSKDIIKFT SEKLSVDEVS
QLVISPLCGA ISLFVGTTRN NFEGKKVISL EYEAYLPMAE NEIRKICSDM RQKWPVRHIA
VFHRLGLVPV TEASVIIAVS SAHRAASLEA VSYAIDALKA RVPIWKKEIY EESSSSWKRN
KECFWATSD
