ID   MOC2B_DROPE             Reviewed;         361 AA.
AC   B4GE20;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=Molybdopterin synthase catalytic subunit {ECO:0000255|HAMAP-Rule:MF_03052};
DE            EC=2.8.1.12 {ECO:0000255|HAMAP-Rule:MF_03052};
DE   AltName: Full=Molybdenum cofactor synthesis protein 2 large subunit {ECO:0000255|HAMAP-Rule:MF_03052};
DE   AltName: Full=Molybdenum cofactor synthesis protein 2B {ECO:0000255|HAMAP-Rule:MF_03052};
DE            Short=MOCS2B {ECO:0000255|HAMAP-Rule:MF_03052};
GN   Name=Mocs2B {ECO:0000250|UniProtKB:Q9VBX2};
GN   Synonyms=Mocs2 {ECO:0000255|HAMAP-Rule:MF_03052}; ORFNames=GL21938;
OS   Drosophila persimilis (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7234;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MSH-3 / Tucson 14011-0111.49;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Catalytic subunit of the molybdopterin synthase complex, a
CC       complex that catalyzes the conversion of precursor Z into
CC       molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms
CC       from thiocarboxylated Mocs2A into precursor Z to generate a dithiolene
CC       group. {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal
CC         Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O = 2
CC         [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly +
CC         4 H(+) + molybdopterin; Xref=Rhea:RHEA:26333, Rhea:RHEA-COMP:12160,
CC         Rhea:RHEA-COMP:12202, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58698, ChEBI:CHEBI:59648, ChEBI:CHEBI:90619,
CC         ChEBI:CHEBI:90778; EC=2.8.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_03052};
CC   -!- PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- SUBUNIT: Heterotetramer; composed of 2 small (Mocs2A) and 2 large
CC       (Mocs2B) subunits. {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- MISCELLANEOUS: This protein is produced by a bicistronic gene which
CC       also produces the small subunit (Mocs2A).
CC   -!- SIMILARITY: Belongs to the MoaE family. MOCS2B subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03052}.
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DR   EMBL; CH479182; EDW33855.1; -; Genomic_DNA.
DR   RefSeq; XP_002016755.1; XM_002016719.1.
DR   AlphaFoldDB; B4GE20; -.
DR   SMR; B4GE20; -.
DR   STRING; 7234.FBpp0186045; -.
DR   PRIDE; B4GE20; -.
DR   EnsemblMetazoa; FBtr0187553; FBpp0186045; FBgn0159531.
DR   GeneID; 6591577; -.
DR   KEGG; dpe:6591577; -.
DR   eggNOG; KOG3307; Eukaryota.
DR   HOGENOM; CLU_045449_0_0_1; -.
DR   OMA; GIAIYHR; -.
DR   PhylomeDB; B4GE20; -.
DR   UniPathway; UPA00344; -.
DR   Proteomes; UP000008744; Unassembled WGS sequence.
DR   GO; GO:0140672; C:ATAC complex; IEA:EnsemblMetazoa.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0019008; C:molybdopterin synthase complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005700; C:polytene chromosome; IEA:EnsemblMetazoa.
DR   GO; GO:0030366; F:molybdopterin synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006338; P:chromatin remodeling; IEA:EnsemblMetazoa.
DR   GO; GO:0016573; P:histone acetylation; IEA:EnsemblMetazoa.
DR   GO; GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0032324; P:molybdopterin cofactor biosynthetic process; IEA:EnsemblMetazoa.
DR   CDD; cd00756; MoaE; 1.
DR   Gene3D; 3.90.1170.40; -; 1.
DR   HAMAP; MF_03052; MOC2B; 1.
DR   InterPro; IPR036563; MoaE_sf.
DR   InterPro; IPR028888; MOCS2B_euk.
DR   InterPro; IPR003448; Mopterin_biosynth_MoaE.
DR   Pfam; PF02391; MoaE; 1.
DR   SUPFAM; SSF54690; SSF54690; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Molybdenum cofactor biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..361
FT                   /note="Molybdopterin synthase catalytic subunit"
FT                   /id="PRO_0000369338"
FT   BINDING         101..102
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
FT   BINDING         117
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
FT   BINDING         124..126
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
SQ   SEQUENCE   361 AA;  41398 MW;  A8B96B051DA86870 CRC64;
     MNHIKLIDCP IDVTYAINLI SDPSCGASSI FIGTTRDSFQ GKKVVSLAYE AYENMALKEM
     DKICSDLRAT WPDLKHILIY HRLGTVPENE ASVVIAASAP HRSAALKAVT FAIDQLKSRV
     PIWKKELYEG NHDAEWKENS ESIRPKKSLS SFNYSVCKVD ESRVVSRNLV QIRANDCELK
     NRVECFFKRK RAEINSCNVI DFKQSNLSSN INSDTEVDVS CARTQSTISK QEQSNCHLKV
     RRATNRCGPQ QMQFRPKYKH ELSRLTTSRV STNEVGESLQ NSRLHSIETY MGLTSKNNEN
     IINRIKNVEN RILLLESTSP EYQHFFEQSC MDQPEKKIKT NKTYSAHELR VYIHRKNKEC
     P