ID   MOC2B_DROWI             Reviewed;         372 AA.
AC   B4NJW0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Molybdopterin synthase catalytic subunit {ECO:0000255|HAMAP-Rule:MF_03052};
DE            EC=2.8.1.12 {ECO:0000255|HAMAP-Rule:MF_03052};
DE   AltName: Full=Molybdenum cofactor synthesis protein 2 large subunit {ECO:0000255|HAMAP-Rule:MF_03052};
DE   AltName: Full=Molybdenum cofactor synthesis protein 2B {ECO:0000255|HAMAP-Rule:MF_03052};
DE            Short=MOCS2B {ECO:0000255|HAMAP-Rule:MF_03052};
GN   Name=Mocs2B {ECO:0000250|UniProtKB:Q9VBX2};
GN   Synonyms=Mocs2 {ECO:0000255|HAMAP-Rule:MF_03052}; ORFNames=GK13883;
OS   Drosophila willistoni (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7260;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tucson 14030-0811.24;
RX   PubMed=17994087; DOI=10.1038/nature06341;
RG   Drosophila 12 genomes consortium;
RT   "Evolution of genes and genomes on the Drosophila phylogeny.";
RL   Nature 450:203-218(2007).
CC   -!- FUNCTION: Catalytic subunit of the molybdopterin synthase complex, a
CC       complex that catalyzes the conversion of precursor Z into
CC       molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms
CC       from thiocarboxylated Mocs2A into precursor Z to generate a dithiolene
CC       group. {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal
CC         Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O = 2
CC         [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly +
CC         4 H(+) + molybdopterin; Xref=Rhea:RHEA:26333, Rhea:RHEA-COMP:12160,
CC         Rhea:RHEA-COMP:12202, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58698, ChEBI:CHEBI:59648, ChEBI:CHEBI:90619,
CC         ChEBI:CHEBI:90778; EC=2.8.1.12; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_03052};
CC   -!- PATHWAY: Cofactor biosynthesis; molybdopterin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- SUBUNIT: Heterotetramer; composed of 2 small (Mocs2A) and 2 large
CC       (Mocs2B) subunits. {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03052}.
CC   -!- MISCELLANEOUS: This protein is produced by a bicistronic gene which
CC       also produces the small subunit (Mocs2A).
CC   -!- SIMILARITY: Belongs to the MoaE family. MOCS2B subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03052}.
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DR   EMBL; CH964272; EDW83962.1; -; Genomic_DNA.
DR   RefSeq; XP_002072976.1; XM_002072940.2.
DR   AlphaFoldDB; B4NJW0; -.
DR   SMR; B4NJW0; -.
DR   STRING; 7260.FBpp0243026; -.
DR   EnsemblMetazoa; FBtr0244534; FBpp0243026; FBgn0215890.
DR   GeneID; 6651376; -.
DR   KEGG; dwi:6651376; -.
DR   eggNOG; KOG3307; Eukaryota.
DR   HOGENOM; CLU_045449_0_0_1; -.
DR   InParanoid; B4NJW0; -.
DR   OMA; GIAIYHR; -.
DR   OrthoDB; 1543738at2759; -.
DR   PhylomeDB; B4NJW0; -.
DR   UniPathway; UPA00344; -.
DR   Proteomes; UP000007798; Unassembled WGS sequence.
DR   GO; GO:0140672; C:ATAC complex; IEA:EnsemblMetazoa.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0019008; C:molybdopterin synthase complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0005700; C:polytene chromosome; IEA:EnsemblMetazoa.
DR   GO; GO:0030366; F:molybdopterin synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006338; P:chromatin remodeling; IEA:EnsemblMetazoa.
DR   GO; GO:0016573; P:histone acetylation; IEA:EnsemblMetazoa.
DR   GO; GO:0006777; P:Mo-molybdopterin cofactor biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0032324; P:molybdopterin cofactor biosynthetic process; IEA:EnsemblMetazoa.
DR   CDD; cd00756; MoaE; 1.
DR   Gene3D; 3.90.1170.40; -; 1.
DR   HAMAP; MF_03052; MOC2B; 1.
DR   InterPro; IPR036563; MoaE_sf.
DR   InterPro; IPR028888; MOCS2B_euk.
DR   InterPro; IPR003448; Mopterin_biosynth_MoaE.
DR   Pfam; PF02391; MoaE; 1.
DR   SUPFAM; SSF54690; SSF54690; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Molybdenum cofactor biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..372
FT                   /note="Molybdopterin synthase catalytic subunit"
FT                   /id="PRO_0000369343"
FT   BINDING         101..102
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
FT   BINDING         117
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
FT   BINDING         124..126
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03052"
SQ   SEQUENCE   372 AA;  42310 MW;  499E7A05DCE149F4 CRC64;
     MDHIELISSP LDITTLYTLL GDPGCGASSV FVGTTRDSFE GKKVLSLEYE AYEPMAIKEM
     KTICTDLRAK WPDLKHIVIY HRLGSVPVGE ASVVIATSAP HRLSALESVS LAVDQLKTRV
     PIWKKELYEG DDPAQWKENK ESLRPKHSQV TSNRFNFATC KIEKQNENIS DKLVQIRVHD
     DELSKRVECF VRRKRMEINM LNVRDFTQNQ SLPHVLNNEE AEVSCARTQG AVIKQEQSNC
     HLKVRRVNNK SGPQQMHLRP NYAQELNKLM GSSESSCDPS GQVQNDLANN RLRTIESYMG
     LSANNQSLLS RLKQVENRIL LLESTSPEYN HFTNNLKQPD SEEPSPKRLK RKIYCAEELN
     NLLKSFKDET GS