MOD5P_SCHPO
ID MOD5P_SCHPO Reviewed; 522 AA.
AC O59740;
DT 14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Cell polarity protein mod5;
DE AltName: Full=Tea1-anchoring protein mod5;
GN Name=mod5; ORFNames=SPBC530.04;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF CYS-519.
RX PubMed=12789340; DOI=10.1038/nature01672;
RA Snaith H.A., Sawin K.E.;
RT "Fission yeast mod5p regulates polarized growth through anchoring of tea1p
RT at cell tips.";
RL Nature 423:647-651(2003).
RN [3]
RP FUNCTION, AND INTERACTION WITH TEA1 AND TEA3.
RX PubMed=16222337; DOI=10.1038/sj.emboj.7600838;
RA Snaith H.A., Samejima I., Sawin K.E.;
RT "Multistep and multimode cortical anchoring of tea1p at cell tips in
RT fission yeast.";
RL EMBO J. 24:3690-3699(2005).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-43; SER-303 AND SER-350, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=18257517; DOI=10.1021/pr7006335;
RA Wilson-Grady J.T., Villen J., Gygi S.P.;
RT "Phosphoproteome analysis of fission yeast.";
RL J. Proteome Res. 7:1088-1097(2008).
CC -!- FUNCTION: With tea1, acts in a positive-feedback loop in the
CC microtubule-mediated regulation of cell polarity. Involved in the
CC anchoring of tea1 at the cortex as well as the correct localization of
CC tea3. {ECO:0000269|PubMed:12789340, ECO:0000269|PubMed:16222337}.
CC -!- SUBUNIT: Interacts with tea1 and tea3. {ECO:0000269|PubMed:16222337}.
CC -!- INTERACTION:
CC O59740; O14248: tea3; NbExp=3; IntAct=EBI-875310, EBI-875326;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12789340};
CC Peripheral membrane protein {ECO:0000269|PubMed:12789340}; Cytoplasmic
CC side {ECO:0000269|PubMed:12789340}. Note=localizes to the cell tips.
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DR EMBL; CU329671; CAA19170.1; -; Genomic_DNA.
DR PIR; T40520; T40520.
DR RefSeq; NP_595317.1; NM_001021224.2.
DR AlphaFoldDB; O59740; -.
DR BioGRID; 277410; 14.
DR IntAct; O59740; 2.
DR STRING; 4896.SPBC530.04.1; -.
DR iPTMnet; O59740; -.
DR MaxQB; O59740; -.
DR PaxDb; O59740; -.
DR PRIDE; O59740; -.
DR EnsemblFungi; SPBC530.04.1; SPBC530.04.1:pep; SPBC530.04.
DR GeneID; 2540894; -.
DR KEGG; spo:SPBC530.04; -.
DR PomBase; SPBC530.04; mod5.
DR VEuPathDB; FungiDB:SPBC530.04; -.
DR HOGENOM; CLU_535461_0_0_1; -.
DR OMA; MKSAATY; -.
DR PRO; PR:O59740; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0098753; C:anchored component of the cytoplasmic side of the plasma membrane; IDA:PomBase.
DR GO; GO:0051285; C:cell cortex of cell tip; IDA:PomBase.
DR GO; GO:0032153; C:cell division site; IDA:PomBase.
DR GO; GO:0051286; C:cell tip; IDA:PomBase.
DR GO; GO:0031520; C:plasma membrane of cell tip; IC:PomBase.
DR GO; GO:0043495; F:protein-membrane adaptor activity; IMP:PomBase.
DR GO; GO:0097248; P:maintenance of protein location in cell cortex of cell tip; IMP:PomBase.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphoprotein; Reference proteome.
FT CHAIN 1..522
FT /note="Cell polarity protein mod5"
FT /id="PRO_0000351429"
FT REGION 1..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 170..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 251..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..76
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 316..348
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 359..446
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 497..515
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 43
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 303
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MOD_RES 350
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18257517"
FT MUTAGEN 519
FT /note="C->S: Prevents normal localization at the cell
FT tips."
FT /evidence="ECO:0000269|PubMed:12789340"
SQ SEQUENCE 522 AA; 56542 MW; AC84C95009BDB64F CRC64;
MSALSESPAI PSFWRPETSE ISKKPRPNTT VGFQFDNRNV GTSAPSTPAI RRNNTDSFER
GLSLPLPSSK QDTGSSVLDP DGDAYVNRYA RPVTAGSIYI PSNYHKSFSP NTFSGFNVKR
SASKSPKRSA NGSTSEDISI EGSPSETAKG ARSSFNSNFR TFDIGSERRR RILEASQDSS
RPGRYSYRTK SASPALIDTS TLDSRLNFTM GRLERSIAQL SKNTMRAVSH LENPPKDITL
PKLNVKNSAW PLQPYSPPAN ETPASSSSSA KARPVSVPDM SSPVPASSVE YESLKAAVTY
SPSQNPKKVA ETDSESRKSS FQSSYNDADR PFQVGAQTQS TPNRISRSDS PIVYDVDTHS
EDNASTASSE AISQSMRSFQ PQPNTGSPFP RFTSTNTEDE QESDIPQSDA NDSTVNLNQP
NYANLTPTPQ VSPKRPTYSR SSPLPSASVP ALGDGSPDPP AAPSIQNSLS VHESEMPPHV
TRDYTQPAAS ATPVPKEKPS EKSEKPPKKK GSKLEKFCCI LM
