MODE_YERPE
ID MODE_YERPE Reviewed; 263 AA.
AC P58497; Q0WHQ6;
DT 19-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT 19-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Transcriptional regulator ModE;
GN Name=modE; OrderedLocusNames=YPO1143, y3040, YP_1017;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: The ModE-Mo complex acts as a repressor of the modABC operon,
CC involved in the transport of molybdate. Upon binding molybdate, the
CC conformation of the protein changes, promoting dimerization of ModE-Mo.
CC The protein dimer is then competent to bind a DNA region, upstream of
CC the modABC operon. Acts also as an enhancer of the expression of genes
CC coding for molybdoenzymes, both directly and indirectly (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the ModE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM86590.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL590842; CAL19807.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM86590.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE017042; AAS61268.1; -; Genomic_DNA.
DR PIR; AE0140; AE0140.
DR RefSeq; WP_002210754.1; NZ_WUCM01000016.1.
DR RefSeq; YP_002346182.1; NC_003143.1.
DR AlphaFoldDB; P58497; -.
DR SMR; P58497; -.
DR IntAct; P58497; 5.
DR STRING; 214092.YPO1143; -.
DR PaxDb; P58497; -.
DR DNASU; 1147986; -.
DR EnsemblBacteria; AAM86590; AAM86590; y3040.
DR EnsemblBacteria; AAS61268; AAS61268; YP_1017.
DR GeneID; 66842392; -.
DR KEGG; ype:YPO1143; -.
DR KEGG; ypk:y3040; -.
DR KEGG; ypm:YP_1017; -.
DR PATRIC; fig|214092.21.peg.1438; -.
DR eggNOG; COG2005; Bacteria.
DR eggNOG; COG3585; Bacteria.
DR HOGENOM; CLU_087839_0_0_6; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0032991; C:protein-containing complex; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0030151; F:molybdenum ion binding; IEA:InterPro.
DR GO; GO:0015689; P:molybdate ion transport; IEA:InterPro.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR InterPro; IPR016462; ModE.
DR InterPro; IPR003725; ModE-bd_N.
DR InterPro; IPR004606; Mop_domain.
DR InterPro; IPR005116; Transp-assoc_OB_typ1.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03459; TOBE; 2.
DR PIRSF; PIRSF005763; Txn_reg_ModE; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR SUPFAM; SSF50331; SSF50331; 2.
DR TIGRFAMs; TIGR00637; ModE_repress; 1.
DR TIGRFAMs; TIGR00638; Mop; 1.
DR PROSITE; PS51866; MOP; 2.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; DNA-binding; Molybdenum; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation; Transport.
FT CHAIN 1..263
FT /note="Transcriptional regulator ModE"
FT /id="PRO_0000201129"
FT DOMAIN 123..190
FT /note="Mop 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01213"
FT DOMAIN 195..261
FT /note="Mop 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01213"
FT DNA_BIND 33..79
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT REGION 1..120
FT /note="I"
FT REGION 124..132
FT /note="Required for dimer formation and molybdate binding"
FT /evidence="ECO:0000250"
SQ SEQUENCE 263 AA; 28298 MW; 10BE42A74D4830AF CRC64;
MQAEILLTLK LQQRLFADPR RIALLKQIQH TGSISQGAKL AGISYKSAWD AINDMNTLSE
EILVERATGG KGGGGAHLTR YGERLIQLYD LLATIQQKAF DTLKDDSLPL DSLLAAISRF
SLQTSARNQF FGTLIERDHQ QVQQHVNILL SDGKTRLTAA ITQQSADRLQ LSAGKEVLAL
IKAPWVKLVT DPALAGAADN ALPGTVASIE PGNDHSEVIV TLTGGANLCS TQNNSELHAL
NLRVGSAVIA QFNADRVIIA TLC
