MOF_ORYSJ
ID MOF_ORYSJ Reviewed; 475 AA.
AC Q7X7A4;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=MEIOTIC F-BOX protein MOF {ECO:0000303|PubMed:27436711};
GN Name=MOF {ECO:0000303|PubMed:27436711};
GN OrderedLocusNames=Os04g0464966 {ECO:0000312|EMBL:BAH92699.1};
GN ORFNames=OSJNBa0060P14.14 {ECO:0000312|EMBL:CAE04361.2},
GN OSJNBb0048E02.5 {ECO:0000312|EMBL:CAE04825.2};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947 {ECO:0000312|EMBL:CAE04361.2};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare; TISSUE=Leaf {ECO:0000312|EMBL:BAH00958.1};
RG The rice full-length cDNA consortium;
RT "Oryza sativa full length cDNA.";
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION, INTERACTION WITH SKP1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND PHYLOGENETIC ANALYSIS.
RC STRAIN=cv. 9522 {ECO:0000303|PubMed:27436711};
RX PubMed=27436711; DOI=10.1105/tpc.16.00108;
RA He Y., Wang C., Higgins J.D., Yu J., Zong J., Lu P., Zhang D., Liang W.;
RT "MEIOTIC F-BOX is essential for male meiotic DNA double-strand break repair
RT in rice.";
RL Plant Cell 28:1879-1893(2016).
CC -!- FUNCTION: Probable component of a SCF (SKP1-CULLIN-F-box protein) E3
CC ubiquitin-protein ligase complex and may function through the
CC ubiquitin-mediated protein degradation or signaling pathway. Required
CC for male meiotic prophase I progression. Required for telomere bouquet
CC formation, homologous chromosome pairing and for the formation of the
CC synaptonemal complex (SC), which stabilizes initial chromosomal axial
CC associations and promotes crossover formation. Involved in meiotic DNA
CC double-strand break (DSB) end-processing and repair, and is important
CC in the recruitment of DSB repair proteins to the DSB sites.
CC {ECO:0000269|PubMed:27436711}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000305|PubMed:27436711}.
CC -!- SUBUNIT: Part of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein
CC ligase complex (Probable). Interacts (via F-box domain) directly with
CC SKP1 (PubMed:27436711). {ECO:0000269|PubMed:27436711,
CC ECO:0000305|PubMed:27436711}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:27436711}. Chromosome
CC {ECO:0000269|PubMed:27436711}. Note=Localizes to male meiotic
CC chromosomes from leptotene to pachytene during meiotic prophase I.
CC {ECO:0000269|PubMed:27436711}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the stem, leaf and in the
CC anther during meiosis. Weakly expressed in roots and lemma/palea.
CC {ECO:0000269|PubMed:27436711}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in the anther during meiosis and
CC then gradually decreases after meiosis. Predominantly expressed during
CC the cytological substages of meiotic prophase I from leptotene to
CC pachytene peaking in zygotene. From stage 7 to stage 9, detected in
CC both the tapetum and male meiocytes of the anther.
CC {ECO:0000269|PubMed:27436711}.
CC -!- DISRUPTION PHENOTYPE: Plants are female fertile, but male sterile as
CC they are not able to produce tetrads and microspores. No differences
CC compared to the wild-type plant during the vegetative stage. Plants
CC develop normal panicles and floral organs, except for smaller and pale-
CC yellow anthers that fail to produce viable pollen grains. No effect on
CC the differentiation or early mitotic division of germ cells. No
CC meiocytes are detected in 4.5 mm and 5.0 mm long flowers, but in 3.0 mm
CC flowers meiosis appears to initiate normally. At leptotene stage of
CC meiotic prophase I, chromosomes appear normal in male meiocytes and
CC cohesion of sister chromatids is not affected. However, subsequent
CC stages of prohase I are severely impaired. Meiocytes display disrupted
CC telomere bouquet formation, impaired pairing and synapsis of homologous
CC chromosomes, and arrested meiocytes at late prophase I, followed by
CC apoptosis. The transverse filament protein ZEP1, an essential
CC synaptonemal complex (SC)-related protein, is not detected. Although
CC normal, programmed DNA double-strand breaks (DSBs) form, foci of the
CC phosphorylated histone gamma H2AX, a marker for DSBs, persist
CC indicating that many of the DSBs remain unrepaired. The recruitment of
CC COM1 and RAD51C to DSBs is severely compromised in meiocytes indicating
CC that normally formed DSB ends are not processed and repaired.
CC {ECO:0000269|PubMed:27436711}.
CC -!- SIMILARITY: Belongs to the F-box protein family. FBX subfamily.
CC {ECO:0000305}.
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DR EMBL; AL606653; CAE04825.2; -; Genomic_DNA.
DR EMBL; AL663017; CAE04361.2; -; Genomic_DNA.
DR EMBL; AP008210; BAH92699.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS89592.1; -; Genomic_DNA.
DR EMBL; AK241116; BAH00958.1; -; mRNA.
DR RefSeq; XP_015634097.1; XM_015778611.1.
DR AlphaFoldDB; Q7X7A4; -.
DR STRING; 4530.OS04T0464966-01; -.
DR PaxDb; Q7X7A4; -.
DR PRIDE; Q7X7A4; -.
DR EnsemblPlants; Os04t0464966-01; Os04t0464966-01; Os04g0464966.
DR GeneID; 9266478; -.
DR Gramene; Os04t0464966-01; Os04t0464966-01; Os04g0464966.
DR KEGG; osa:9266478; -.
DR eggNOG; ENOG502T11G; Eukaryota.
DR HOGENOM; CLU_003068_3_0_1; -.
DR InParanoid; Q7X7A4; -.
DR OMA; QRWIRRA; -.
DR OrthoDB; 686125at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0005694; C:chromosome; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0051321; P:meiotic cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF00646; F-box; 1.
DR SMART; SM00256; FBOX; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Chromosome; DNA damage; DNA repair; Meiosis; Nucleus; Reference proteome;
KW Ubl conjugation pathway.
FT CHAIN 1..475
FT /note="MEIOTIC F-BOX protein MOF"
FT /id="PRO_0000442779"
FT DOMAIN 55..91
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT REGION 1..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 35..55
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 475 AA; 53064 MW; 63345B3CB2D4CAA5 CRC64;
MRRERDATQI PENPMEGIPQ TAAAAAAAAA AEASEPPRKR ARVDGGGGGA GEEEEDRLSD
LPDCLLEDIL AHLGSRQAVQ TSVLSRRWRN LWRGVRVVVI DVGSFRLPGA DGDPPRFRLD
RIEDFADGVL SPSLHPGAAR ELDALRMRLD EDAVTTNFQR WIRRALWRRP ATVDLYYLPR
RSFSWPPAVP LTPVTAVSRL KTLRIFGLRP TVVFGADEFP ALEDLHIERC SYAHGTIASP
TLKRLALVSP INGCFVREQR LTAPGLTSLR LVLPYSREEG VRVITDAPLT SLVDASITIV
DTDPGDPRNR RVNQFKVDFL VAISNLLGRL TSVRNLDLTG LNATALLDNK SQEFPMFPYL
TTLLLNECDI GYKYHVLRSI LQNAPNLEQL RLHNCKFVGK SRRKAGQTQS KEKTSKCSSS
TLSSACSSLK SVEIKHPRGE PSHDLLHEFL KEIPHNQWRK RSIDEETISI ELNRK
