ID   ARND_ERWT9              Reviewed;         296 AA.
AC   B2VBI8;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD {ECO:0000255|HAMAP-Rule:MF_01870};
DE            EC=3.5.1.n3 {ECO:0000255|HAMAP-Rule:MF_01870};
GN   Name=arnD {ECO:0000255|HAMAP-Rule:MF_01870}; OrderedLocusNames=ETA_23800;
OS   Erwinia tasmaniensis (strain DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB
OS   4357 / Et1/99).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Erwinia.
OX   NCBI_TaxID=465817;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17950 / CFBP 7177 / CIP 109463 / NCPPB 4357 / Et1/99;
RX   PubMed=18462403; DOI=10.1111/j.1462-2920.2008.01639.x;
RA   Kube M., Migdoll A.M., Mueller I., Kuhl H., Beck A., Reinhardt R.,
RA   Geider K.;
RT   "The genome of Erwinia tasmaniensis strain Et1/99, a non-pathogenic
RT   bacterium in the genus Erwinia.";
RL   Environ. Microbiol. 10:2211-2222(2008).
CC   -!- FUNCTION: Catalyzes the deformylation of 4-deoxy-4-formamido-L-
CC       arabinose-phosphoundecaprenol to 4-amino-4-deoxy-L-arabinose-
CC       phosphoundecaprenol. The modified arabinose is attached to lipid A and
CC       is required for resistance to polymyxin and cationic antimicrobial
CC       peptides. {ECO:0000255|HAMAP-Rule:MF_01870}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4-deoxy-4-formamido-alpha-L-arabinopyranosyl di-trans,octa-
CC         cis-undecaprenyl phosphate + H2O = 4-amino-4-deoxy-alpha-L-
CC         arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + formate;
CC         Xref=Rhea:RHEA:27734, ChEBI:CHEBI:15377, ChEBI:CHEBI:15740,
CC         ChEBI:CHEBI:58909, ChEBI:CHEBI:60463; EC=3.5.1.n3;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01870};
CC   -!- PATHWAY: Glycolipid biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose
CC       undecaprenyl phosphate biosynthesis; 4-amino-4-deoxy-alpha-L-arabinose
CC       undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose
CC       and undecaprenyl phosphate: step 2/2. {ECO:0000255|HAMAP-
CC       Rule:MF_01870}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01870}.
CC   -!- SIMILARITY: Belongs to the polysaccharide deacetylase family. ArnD
CC       deformylase subfamily. {ECO:0000255|HAMAP-Rule:MF_01870}.
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DR   EMBL; CU468135; CAO97426.1; -; Genomic_DNA.
DR   RefSeq; WP_012442094.1; NC_010694.1.
DR   AlphaFoldDB; B2VBI8; -.
DR   STRING; 465817.ETA_23800; -.
DR   EnsemblBacteria; CAO97426; CAO97426; ETA_23800.
DR   KEGG; eta:ETA_23800; -.
DR   eggNOG; COG0726; Bacteria.
DR   HOGENOM; CLU_084199_0_0_6; -.
DR   OMA; KFLWKML; -.
DR   OrthoDB; 1701876at2; -.
DR   UniPathway; UPA00030; -.
DR   UniPathway; UPA00036; UER00496.
DR   Proteomes; UP000001726; Chromosome.
DR   GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:UniProtKB-UniRule.
DR   GO; GO:0036108; P:4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   HAMAP; MF_01870; ArnD; 1.
DR   InterPro; IPR023557; ArnD.
DR   InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR   InterPro; IPR002509; NODB_dom.
DR   Pfam; PF01522; Polysacc_deac_1; 1.
DR   SUPFAM; SSF88713; SSF88713; 1.
DR   PROSITE; PS51677; NODB; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Hydrolase; Lipid A biosynthesis; Lipid biosynthesis;
KW   Lipid metabolism; Lipopolysaccharide biosynthesis; Reference proteome.
FT   CHAIN           1..296
FT                   /note="Probable 4-deoxy-4-formamido-L-arabinose-
FT                   phosphoundecaprenol deformylase ArnD"
FT                   /id="PRO_0000383494"
FT   DOMAIN          2..260
FT                   /note="NodB homology"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01870"
SQ   SEQUENCE   296 AA;  33122 MW;  072DF65CA390A8CD CRC64;
     MIDVGLRIDV DTWRGTRDGV PNLLSILQRQ QTRGSFFFSV GPDNMGRHLW RLLKPRFLWK
     MLRSNAASLY GWDILLAGTA WPGRRIGQGQ AEVIRATARQ HEIGLHAWDH FAWQTWAGVW
     PAERLSAEIR RGVEALADIV GQPVDCSAVA GWRADGQVVA AKQPFDFTYN SDCRGVRPFR
     PQLPDGSLGT VQIPVTLPTW DEVIGSSVTR EGYNDFILDQ ITQHQGVPVY TIHAEVEGIV
     LSEMFERLLV EAGRRGIRFC PLGELLPQDV TQLPVGKIVR GHNPGREGWL GCQQLS