MOGT1_XENLA
ID MOGT1_XENLA Reviewed; 335 AA.
AC Q3KPP4; Q7ZXC3;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=2-acylglycerol O-acyltransferase 1;
DE EC=2.3.1.22;
DE AltName: Full=Acyl-CoA:monoacylglycerol acyltransferase 1;
DE Short=MGAT1;
DE AltName: Full=Monoacylglycerol O-acyltransferase 1;
GN Name=mogat1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Embryo;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of diacylglycerol from 2-
CC monoacylglycerol and fatty acyl-CoA. Probably not involved in
CC absorption of dietary fat in the small intestine.
CC {ECO:0000250|UniProtKB:Q91ZV4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2-acylglycerol + an acyl-CoA = a 1,2-diacyl-sn-glycerol +
CC CoA; Xref=Rhea:RHEA:32947, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:58342; EC=2.3.1.22;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:32948;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 2-(9Z-octadecenoyl)-glycerol = 1,2-di-
CC (9Z-octadecenoyl)-sn-glycerol + CoA; Xref=Rhea:RHEA:37911,
CC ChEBI:CHEBI:52333, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:73990; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37912;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z-octadecenoyl)-glycerol = 1,2-di-
CC (9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:37915,
CC ChEBI:CHEBI:52323, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75342; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:37916;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + butanoyl-CoA = 1-butyryl-2-
CC (9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38051,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57371, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75443; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38052;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + octanoyl-CoA = 1-octanoyl-2-
CC (9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38059,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57386, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75462; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38060;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + dodecanoyl-CoA = 1-dodecanoyl-
CC 2-(9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38063,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57375, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75463; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38064;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + tetradecanoyl-CoA = 1-
CC tetradecanoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38067, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75465;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38068;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + hexadecanoyl-CoA = 1-
CC hexadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38071, ChEBI:CHEBI:57287, ChEBI:CHEBI:57379,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75466;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38072;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + octadecanoyl-CoA = 1-
CC octadecanoyl-2-(9Z-octadecenoyl)-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38075, ChEBI:CHEBI:57287, ChEBI:CHEBI:57394,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75468;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38076;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(9Z-octadecenoyl)-glycerol + eicosanoyl-CoA = 1-eicosanoyl-
CC 2-(9Z)-octadecenoyl-sn-glycerol + CoA; Xref=Rhea:RHEA:38079,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57380, ChEBI:CHEBI:73990,
CC ChEBI:CHEBI:75470; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38080;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z,12Z)-octadecadienoyl-CoA + 2-(9Z-octadecenoyl)-glycerol =
CC 1-(9Z,12Z)-octadecadienoyl-2-(9Z)-octadecenoyl-sn-glycerol + CoA;
CC Xref=Rhea:RHEA:38083, ChEBI:CHEBI:57287, ChEBI:CHEBI:57383,
CC ChEBI:CHEBI:73990, ChEBI:CHEBI:75471;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38084;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 2-(9Z-octadecenoyl)-
CC glycerol = 1-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-2-(9Z)-octadecenoyl-sn-
CC glycerol + CoA; Xref=Rhea:RHEA:38087, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57368, ChEBI:CHEBI:73990, ChEBI:CHEBI:75472;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38088;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-dodecanoylglycerol = 1-dodecanoyl-2-
CC (9Z-octadecenoyl)-glycerol + CoA; Xref=Rhea:RHEA:38115,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:75539,
CC ChEBI:CHEBI:75579; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38116;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-tetradecanoylglycerol = 1-
CC tetradecanoyl-2-(9Z-octadecenoyl)-glycerol + CoA;
CC Xref=Rhea:RHEA:38119, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75562, ChEBI:CHEBI:75582;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38120;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-hexadecanoylglycerol = 1-
CC hexadecanoyl-2-(9Z-octadecenoyl)glycerol + CoA; Xref=Rhea:RHEA:38123,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:69081,
CC ChEBI:CHEBI:75585; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38124;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-octadecanoylglycerol = 1-
CC octadecanoyl-2-(9Z)-octadecenoylglycerol + CoA; Xref=Rhea:RHEA:38127,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57387, ChEBI:CHEBI:75555,
CC ChEBI:CHEBI:75590; Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38128;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z-octadecadienoyl)-glycerol =
CC 1-(9Z,12Z-octadecadienoyl)-2-(9Z-octadecenoyl)-glycerol + CoA;
CC Xref=Rhea:RHEA:38131, ChEBI:CHEBI:57287, ChEBI:CHEBI:57387,
CC ChEBI:CHEBI:75568, ChEBI:CHEBI:75614;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38132;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(9Z,12Z,15Z-octadecatrienoyl)-
CC glycerol = 1-(9Z,12Z,15Z-octadecatrienoyl)-2-(9Z-octadecenoyl)-
CC glycerol + CoA; Xref=Rhea:RHEA:38135, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:75609, ChEBI:CHEBI:75610;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38136;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(9Z)-octadecenoyl-CoA + 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-
CC glycerol = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-2-(9Z-octadecenoyl)-
CC glycerol + CoA; Xref=Rhea:RHEA:38139, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57387, ChEBI:CHEBI:75611, ChEBI:CHEBI:75612;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38140;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecane-1,2-diol + hexadecanoyl-CoA = 2-hydroxyhexadecyl
CC hexadecanoate + CoA; Xref=Rhea:RHEA:38171, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57379, ChEBI:CHEBI:75586, ChEBI:CHEBI:75587;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38172;
CC Evidence={ECO:0000250|UniProtKB:Q91ZV4};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:Q91ZV4}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q91ZV4}.
CC -!- SIMILARITY: Belongs to the diacylglycerol acyltransferase family.
CC {ECO:0000305}.
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DR EMBL; BC045058; AAH45058.1; -; mRNA.
DR EMBL; BC106627; AAI06628.1; -; mRNA.
DR RefSeq; NP_001080699.1; NM_001087230.1.
DR AlphaFoldDB; Q3KPP4; -.
DR DNASU; 380391; -.
DR GeneID; 380391; -.
DR KEGG; xla:380391; -.
DR CTD; 380391; -.
DR Xenbase; XB-GENE-1004231; mogat1.L.
DR OrthoDB; 1347007at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000186698; Chromosome 5L.
DR Bgee; 380391; Expressed in stomach and 19 other tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0003846; F:2-acylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR007130; DAGAT.
DR Pfam; PF03982; DAGAT; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Endoplasmic reticulum; Glycerol metabolism; Glycoprotein;
KW Lipid biosynthesis; Lipid metabolism; Membrane; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..335
FT /note="2-acylglycerol O-acyltransferase 1"
FT /id="PRO_0000249060"
FT TRANSMEM 24..44
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 125
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 180
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 220
FT /note="Y -> H (in Ref. 1; AAH45058)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 335 AA; 38625 MW; 84AE02E094F36D42 CRC64;
MKVEFAPLNI PLARRLQTTA VFQWVFSFLL LAQCCIGIFI CLVLARVWLL LALYVLWLYL
DWETPQAGGR RWEWVRNWPV WKYFKDYFPI RLVKTCDLDP QHNYIMGFHP HGVLVAGAFG
NFCTNYTGFK ELFPGLTPYL HILPFWFRCP FFREYIMSVG LVSASKKSVN HVLSKEDGGN
VSIIVTGGAE ESLDAHPGSL TLNILKRKGF IKVALKRGAY LVPVFSFGEN ELFQQVSNPK
GSLLRCVQER LQRIMGLAMP LFHARGIFQY SFGLMPYRMP IHTVVGRPIP VKETSHPTQE
EIESLHQQYL GALQELFEEH KKRYGIPEHE SLIFI
