ID   MON1_ASHGO              Reviewed;         581 AA.
AC   Q75EA2;
DT   06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 100.
DE   RecName: Full=Vacuolar fusion protein MON1;
GN   Name=MON1; OrderedLocusNames=AAR175C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: In complex with CCZ1, is required for multiple vacuole
CC       delivery pathways including the cytoplasm to vacuole transport (Cvt),
CC       autophagy, pexophagy and endocytosis. The CCZ1-MON1 complex acts at the
CC       fusion of vesicles with the vacuole, through its regulation of the
CC       SNARE complex during the coordinated priming and docking stages of
CC       fusion, and particularly at the stage of tethering/docking.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endosome, multivesicular body membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Prevacuolar
CC       compartment membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}. Vacuole membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the MON1/SAND family. {ECO:0000305}.
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DR   EMBL; AE016814; AAS50542.2; -; Genomic_DNA.
DR   RefSeq; NP_982718.2; NM_208071.2.
DR   AlphaFoldDB; Q75EA2; -.
DR   STRING; 33169.AAS50542; -.
DR   EnsemblFungi; AAS50542; AAS50542; AGOS_AAR175C.
DR   GeneID; 4618754; -.
DR   KEGG; ago:AGOS_AAR175C; -.
DR   eggNOG; KOG0997; Eukaryota.
DR   HOGENOM; CLU_014574_0_0_1; -.
DR   InParanoid; Q75EA2; -.
DR   OMA; TNSTCEY; -.
DR   Proteomes; UP000000591; Chromosome I.
DR   GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR   GO; GO:0035658; C:Mon1-Ccz1 complex; IBA:GO_Central.
DR   GO; GO:0032585; C:multivesicular body membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:EnsemblFungi.
DR   GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IEA:EnsemblFungi.
DR   GO; GO:0010314; F:phosphatidylinositol-5-phosphate binding; IEA:EnsemblFungi.
DR   GO; GO:0001786; F:phosphatidylserine binding; IEA:EnsemblFungi.
DR   GO; GO:0032258; P:cytoplasm to vacuole transport by the Cvt pathway; IEA:EnsemblFungi.
DR   GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IEA:EnsemblFungi.
DR   GO; GO:0016236; P:macroautophagy; IEA:EnsemblFungi.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IEA:EnsemblFungi.
DR   GO; GO:0044395; P:protein targeting to vacuolar membrane; IEA:EnsemblFungi.
DR   GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR   GO; GO:0048278; P:vesicle docking; IEA:EnsemblFungi.
DR   InterPro; IPR043972; FUZ/MON1/HPS1_longin_1.
DR   InterPro; IPR043971; FUZ/MON1/HPS1_longin_2.
DR   InterPro; IPR043970; FUZ/MON1/HPS1_longin_3.
DR   InterPro; IPR004353; Mon1.
DR   PANTHER; PTHR13027; PTHR13027; 1.
DR   Pfam; PF19036; Fuz_longin_1; 1.
DR   Pfam; PF19037; Fuz_longin_2; 1.
DR   Pfam; PF19038; Fuz_longin_3; 1.
DR   PRINTS; PR01546; YEAST73DUF.
PE   3: Inferred from homology;
KW   Autophagy; Endosome; Membrane; Protein transport; Reference proteome;
KW   Transport; Vacuole.
FT   CHAIN           1..581
FT                   /note="Vacuolar fusion protein MON1"
FT                   /id="PRO_0000278851"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   581 AA;  64719 MW;  AF0B888515A1CD71 CRC64;
     MSTSGSRDSA STRESARETR AGPQALPLGS VVSLSSMRSA SPTHAPWVAK PTTSVDLTNH
     FMEVAARGGE EERSLASPGE EMQYELAHSM KAAPALPREL PSPFRAAGGR GALPQEKQFF
     VLTAAGKPIY SMHGPEELVM GLMGIAHTLL NYFELDGPRE RLRTITTYDA HGVLQRFAFL
     RKKHVVLLAM TTRNEAEAEL QQQLDLLYSY LVSSLSLRQM SRLFERANFD LRSFLTESDF
     HNLDQLCRAM CDGTHLGWTL GALECITLKH SVRQRINSIM LRATRGLAVG TLLYGMIVAP
     DHRLVSVLRP KGHTLHTTDL QLLFSMVENQ LQLLHSSQEV WVPLCFPKFN GNGFLYCYIK
     FLPTDTDSAS NETTLEGNMH IKPTLILISP QKSSFYDLHT CASEILDNLA VAGIMPHITA
     PSRFTINDIP APLVHHFIYK SKKYVQYVMP ETSSTTDWST LMKYYSHLRS NALDSHGNPL
     SENVVAFARW CASSGQVQVE YCDSSEELQV EDQPSSIFSE SFVEESLNMT GLSWITPSFE
     LYLLCNNGEI PKAVVMKSAR NVVGWCSRNG HKLFVSEGAV F