MONB_DIOCU
ID MONB_DIOCU Reviewed; 50 AA.
AC P02882;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Monellin chain B;
DE AltName: Full=Monellin chain II;
OS Dioscoreophyllum cumminsii (Serendipity berry) (Rhopalandria cumminsii).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Menispermaceae;
OC Chasmantheroideae; Burasaieae; Dioscoreophyllum.
OX NCBI_TaxID=3457;
RN [1]
RP PROTEIN SEQUENCE.
RX PubMed=1368575; DOI=10.1271/bbb1961.54.2219;
RA Kohmura M., Nio N., Ariyoshi Y.;
RT "Complete amino acid sequence of the sweet protein monellin.";
RL Agric. Biol. Chem. 54:2219-2224(1990).
RN [2]
RP PROTEIN SEQUENCE.
RX PubMed=964918; DOI=10.1515/bchm2.1976.357.1.585;
RA Frank G., Zuber H.;
RT "The complete amino acid sequences of both subunits of the sweet protein
RT monellin.";
RL Hoppe-Seyler's Z. Physiol. Chem. 357:585-592(1976).
RN [3]
RP PROTEIN SEQUENCE.
RX PubMed=4107; DOI=10.1016/0005-2795(76)90293-2;
RA Bohak Z., Li S.-L.;
RT "The structure of monellin and its relation to the sweetness of the
RT protein.";
RL Biochim. Biophys. Acta 427:153-170(1976).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).
RX PubMed=3614382; DOI=10.1038/328739a0;
RA Ogata C., Hatada M., Tomlinson G., Shin W.-C., Kim S.-H.;
RT "Crystal structure of the intensely sweet protein monellin.";
RL Nature 328:739-742(1987).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
RX PubMed=8230222; DOI=10.1006/jmbi.1993.1594;
RA Somoza J.R., Jiang F., Tong L., Kang C.-H., Cho J.M., Kim S.-H.;
RT "Two crystal structures of a potently sweet protein. Natural monellin at
RT 2.75-A resolution and single-chain monellin at 1.7-A resolution.";
RL J. Mol. Biol. 234:390-404(1993).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
RX PubMed=15299859; DOI=10.1107/s0907444997006860;
RA Bujacz G., Miller M., Harrison R., Thanki N., Gilliland G.L., Ogata C.,
RA Kim S.-H., Wlodawer A.;
RT "Structure of monellin refined to 2.3-A resolution in the orthorhombic
RT crystal form.";
RL Acta Crystallogr. D 53:713-719(1997).
RN [7]
RP SIMILARITY TO CYSTATINS.
RX PubMed=8464079; DOI=10.1006/jmbi.1993.1186;
RA Murzin A.G.;
RT "Sweet-tasting protein monellin is related to the cystatin family of thiol
RT proteinase inhibitors.";
RL J. Mol. Biol. 230:689-694(1993).
CC -!- FUNCTION: Taste-modifying protein; intensely sweet-tasting protein.
CC -!- SUBUNIT: Heterodimer of an A chain and a B chain.
CC -!- BIOTECHNOLOGY: Natural monellin has not been mass marketed to the food
CC industry, primarily due to the challenge of large-scale purification of
CC the protein which is relatively sensitive to heat or acid treatment.
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DR PIR; JH0210; MLDIB.
DR PDB; 1FA3; NMR; -; A=1-48.
DR PDB; 1FUW; NMR; -; A=1-48.
DR PDB; 1IV7; X-ray; 1.82 A; A/B=1-48.
DR PDB; 1IV9; X-ray; 1.90 A; A/B=1-50.
DR PDB; 1KRL; X-ray; 1.90 A; B/D=1-50.
DR PDB; 1M9G; NMR; -; A=1-48.
DR PDB; 1MNL; NMR; -; A=1-48.
DR PDB; 1MOL; X-ray; 1.70 A; A/B=1-48.
DR PDB; 2O9U; X-ray; 1.15 A; X=1-48.
DR PDB; 3MON; X-ray; 2.80 A; B/D/F/H=1-48.
DR PDB; 3PXM; X-ray; 1.80 A; A/B=1-48.
DR PDB; 3PYJ; X-ray; 2.00 A; A=1-48.
DR PDB; 3Q2P; X-ray; 2.34 A; A/B/C/D=1-48.
DR PDB; 4MON; X-ray; 2.30 A; B/D=1-48.
DR PDB; 5LC6; X-ray; 1.70 A; A/B=1-48.
DR PDB; 5LC7; X-ray; 1.55 A; A/B=1-48.
DR PDB; 5O7K; X-ray; 2.05 A; A/B=1-48.
DR PDB; 5O7L; X-ray; 2.60 A; A/B=1-48.
DR PDB; 5O7Q; X-ray; 1.72 A; A/B=1-48.
DR PDB; 5O7R; X-ray; 2.06 A; A/B=1-48.
DR PDB; 5O7S; X-ray; 2.02 A; A/B=1-48.
DR PDB; 5XFU; X-ray; 2.61 A; A/B/C/D/E=1-48.
DR PDB; 5YCT; X-ray; 1.85 A; A/B=1-48.
DR PDB; 5YCU; X-ray; 2.32 A; A/B/C/D/E=1-46.
DR PDB; 5YCW; X-ray; 2.29 A; A=1-46.
DR PDB; 5Z1P; X-ray; 1.89 A; A/B/C/D=1-48.
DR PDB; 6L44; X-ray; 2.49 A; A/B=1-46.
DR PDB; 6L4I; X-ray; 2.20 A; A/B=1-48.
DR PDB; 6L4J; X-ray; 2.30 A; A/B=1-48.
DR PDB; 6L4N; X-ray; 2.43 A; A/B=1-46.
DR PDB; 6LAY; X-ray; 3.00 A; A/B=1-48.
DR PDB; 7D75; X-ray; 2.50 A; A/B/C/D=1-49.
DR PDB; 7EUA; X-ray; 2.43 A; A=1-48.
DR PDBsum; 1FA3; -.
DR PDBsum; 1FUW; -.
DR PDBsum; 1IV7; -.
DR PDBsum; 1IV9; -.
DR PDBsum; 1KRL; -.
DR PDBsum; 1M9G; -.
DR PDBsum; 1MNL; -.
DR PDBsum; 1MOL; -.
DR PDBsum; 2O9U; -.
DR PDBsum; 3MON; -.
DR PDBsum; 3PXM; -.
DR PDBsum; 3PYJ; -.
DR PDBsum; 3Q2P; -.
DR PDBsum; 4MON; -.
DR PDBsum; 5LC6; -.
DR PDBsum; 5LC7; -.
DR PDBsum; 5O7K; -.
DR PDBsum; 5O7L; -.
DR PDBsum; 5O7Q; -.
DR PDBsum; 5O7R; -.
DR PDBsum; 5O7S; -.
DR PDBsum; 5XFU; -.
DR PDBsum; 5YCT; -.
DR PDBsum; 5YCU; -.
DR PDBsum; 5YCW; -.
DR PDBsum; 5Z1P; -.
DR PDBsum; 6L44; -.
DR PDBsum; 6L4I; -.
DR PDBsum; 6L4J; -.
DR PDBsum; 6L4N; -.
DR PDBsum; 6LAY; -.
DR PDBsum; 7D75; -.
DR PDBsum; 7EUA; -.
DR AlphaFoldDB; P02882; -.
DR BMRB; P02882; -.
DR PCDDB; P02882; -.
DR SMR; P02882; -.
DR IntAct; P02882; 1.
DR MINT; P02882; -.
DR EvolutionaryTrace; P02882; -.
DR InterPro; IPR046350; Cystatin_sf.
DR InterPro; IPR015283; Monellin.
DR InterPro; IPR002095; Monellin_B.
DR Pfam; PF09200; Monellin; 1.
DR PRINTS; PR00631; MONELLINB.
DR SUPFAM; SSF54403; SSF54403; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Taste-modifying protein.
FT CHAIN 1..50
FT /note="Monellin chain B"
FT /id="PRO_0000207166"
FT SITE 41
FT /note="Blocking, abolishes the sweet taste"
FT CONFLICT 49..50
FT /note="EN -> NE (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 4..6
FT /evidence="ECO:0007829|PDB:2O9U"
FT HELIX 10..26
FT /evidence="ECO:0007829|PDB:2O9U"
FT TURN 27..29
FT /evidence="ECO:0007829|PDB:5LC7"
FT STRAND 34..47
FT /evidence="ECO:0007829|PDB:2O9U"
SQ SEQUENCE 50 AA; 5835 MW; F08396DFCD480C81 CRC64;
GEWEIIDIGP FTQNLGKFAV DEENKIGQYG RLTFNKVIRP CMKKTIYEEN
