MOONR_MOUSE
ID MOONR_MOUSE Reviewed; 959 AA.
AC Q6A000; Q80XQ5; Q9CUC0;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Protein moonraker {ECO:0000250|UniProtKB:Q2KHM9};
DE Short=MNR {ECO:0000250|UniProtKB:Q2KHM9};
GN Name=Kiaa0753; Synonyms=Mnr {ECO:0000250|UniProtKB:Q2KHM9};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-624.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP INTERACTION WITH CEP20; OFD1 AND PCM1.
RX PubMed=26643951; DOI=10.1093/hmg/ddv488;
RA Chevrier V., Bruel A.L., Van Dam T.J., Franco B., Lo Scalzo M., Lembo F.,
RA Audebert S., Baudelet E., Isnardon D., Bole A., Borg J.P., Kuentz P.,
RA Thevenon J., Burglen L., Faivre L., Riviere J.B., Huynen M.A., Birnbaum D.,
RA Rosnet O., Thauvin-Robinet C.;
RT "OFIP/KIAA0753 forms a complex with OFD1 and FOR20 at pericentriolar
RT satellites and centrosomes and is mutated in one individual with oral-
RT facial-digital syndrome.";
RL Hum. Mol. Genet. 25:497-513(2016).
CC -!- FUNCTION: Involved in centriole duplication. Positively regulates CEP63
CC centrosomal localization. Required for WDR62 centrosomal localization
CC and promotes the centrosomal localization of CDK2. May play a role in
CC cilium assembly. {ECO:0000250|UniProtKB:Q2KHM9}.
CC -!- SUBUNIT: Interacts with CEP63 and WDR62 (By similarity). Forms a
CC complex with OFD1 and CEP20/FOR20 (PubMed:26643951). Interacts with
CC PCM1 (PubMed:26643951). {ECO:0000250|UniProtKB:Q2KHM9,
CC ECO:0000269|PubMed:26643951}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriolar satellite
CC {ECO:0000250|UniProtKB:Q2KHM9}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD32296.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AK173018; BAD32296.1; ALT_INIT; mRNA.
DR EMBL; BX119911; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC043106; AAH43106.1; -; mRNA.
DR EMBL; AK016946; BAB30514.1; -; mRNA.
DR CCDS; CCDS24979.1; -.
DR RefSeq; NP_083239.1; NM_028963.2.
DR RefSeq; XP_006534430.1; XM_006534367.2.
DR RefSeq; XP_006534431.1; XM_006534368.3.
DR RefSeq; XP_006534432.1; XM_006534369.3.
DR RefSeq; XP_006534433.1; XM_006534370.3.
DR RefSeq; XP_006534435.1; XM_006534372.3.
DR RefSeq; XP_017170302.1; XM_017314813.1.
DR AlphaFoldDB; Q6A000; -.
DR SMR; Q6A000; -.
DR STRING; 10090.ENSMUSP00000104146; -.
DR iPTMnet; Q6A000; -.
DR PhosphoSitePlus; Q6A000; -.
DR jPOST; Q6A000; -.
DR MaxQB; Q6A000; -.
DR PaxDb; Q6A000; -.
DR PRIDE; Q6A000; -.
DR DNASU; 74477; -.
DR GeneID; 74477; -.
DR KEGG; mmu:74477; -.
DR UCSC; uc007jyd.1; mouse.
DR MGI; MGI:1921727; 4933427D14Rik.
DR eggNOG; ENOG502QQYJ; Eukaryota.
DR InParanoid; Q6A000; -.
DR OrthoDB; 651659at2759; -.
DR PhylomeDB; Q6A000; -.
DR TreeFam; TF331402; -.
DR BioGRID-ORCS; 74477; 4 hits in 72 CRISPR screens.
DR ChiTaRS; 4933427D14Rik; mouse.
DR PRO; PR:Q6A000; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q6A000; protein.
DR GO; GO:0034451; C:centriolar satellite; ISO:MGI.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0007099; P:centriole replication; IEA:InterPro.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0061824; P:cytosolic ciliogenesis; ISS:UniProtKB.
DR GO; GO:0071539; P:protein localization to centrosome; ISO:MGI.
DR InterPro; IPR031447; MNR.
DR PANTHER; PTHR15732; PTHR15732; 2.
DR Pfam; PF15718; MNR; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..959
FT /note="Protein moonraker"
FT /id="PRO_0000280110"
FT REGION 124..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 456..560
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 572..610
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 692..721
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 848..883
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 877..959
FT /note="Necessary and sufficient for CEP20-binding"
FT /evidence="ECO:0000250|UniProtKB:Q2KHM9"
FT COILED 582..674
FT /evidence="ECO:0000255"
FT COMPBIAS 124..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..535
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 853..876
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 279
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q2KHM9"
FT MOD_RES 691
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q2KHM9"
FT CONFLICT 45
FT /note="R -> S (in Ref. 4; BAB30514)"
FT /evidence="ECO:0000305"
FT CONFLICT 175
FT /note="S -> A (in Ref. 4; BAB30514)"
FT /evidence="ECO:0000305"
FT CONFLICT 192
FT /note="L -> P (in Ref. 4; BAB30514)"
FT /evidence="ECO:0000305"
FT CONFLICT 408
FT /note="H -> P (in Ref. 4; BAB30514)"
FT /evidence="ECO:0000305"
FT CONFLICT 585
FT /note="I -> T (in Ref. 4; BAB30514)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 959 AA; 108875 MW; 18CB8D78964E53D4 CRC64;
MGPSQPASTC VHLAPSLQLD AMSDPRNLQP QNQLQFNRNV PTNPRNLAVR YSCPHGIKIE
KLKHSYNELN HGKDLDFKAG NELSSSVSFS VISEERLSYA VHLAKRDVKR RQFEEHVKDH
LRSLPHSSHK GMHTKVERKD SKSQDVCHCS HQPSRVSLSS SGAKVYVYTS QPGRSDLTVP
NSPPTHDPGL QLQPRIEENK NLWEQKGLLE VQRLQKELSG CIRKIEALTK KDRLEEALDP
DEEHRIRVRR QEQAVRCARM LYVLQQQVKE IQEELDKLSP QKIKHTKKSW AVSRLAAAHR
AAIRALQVFV TQFTDRGEHP VPARCRELGS LIRQLSLCSA KLDADPSVPD VVIDILQQIE
ALESLLEKKL SPKKAKKCFT EIRSRFPVGS QKILERWPTV LPKSERRHLV TKETFPQETS
RPPVAKRLLA AMCQPDGELQ RLESEPDVLD AHLLPEEAPR IEDNGTDFKD GTLTPAKPQA
VRRKALTGSM PIRKKDTVES ARPQQGLHIA ERNRPNQPYS KSRLQQTTVS SRLKMNRQPM
KDHRAPWIPP NPTSPPASPK CAAWMKVKYS PRDAAKEQSL QQEDIHKESQ LRGDAEQEAA
RLSWPDAESS KRLKELEELE AKEMERKQKQ RLNWLEAETS RRTKELDELK AEEMDRLQKL
SVSATQLADK VEEAVLERLK PLLIKAQRVN SSVEANSHLK DRPSRHAAAA AQPAEQASDV
PFESRNIPQL RDCLEDTAHE LWARTQDKIL GSETSARLGD SKDSPDLETM MLRMEEMEKY
QETVRQRYNK IVYADPHLWM HEERNDQNTP AVSEGPLASH PIKITKTATQ KCPAVNILLE
RPCNANSLDE SVTTEEGSEK REAPLPLSRE DLHQRKGQTP LSVPPRMRHS IGAYCSRFEQ
FLRIIAHEAI GSFNPWLIAE SFSDELVDEA LGAVAAELQD VCEDYAEAVF TSEFLEAAA
