ID   MORA_RABIT              Reviewed;         125 AA.
AC   P82810;
DT   17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=Morphine 6-dehydrogenase;
DE            EC=1.1.1.218;
DE   Flags: Fragments;
OS   Oryctolagus cuniculus (Rabbit).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX   NCBI_TaxID=9986;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Liver;
RX   PubMed=9143356; DOI=10.1006/abbi.1997.9961;
RA   Yamano S., Ito K., Ogata S., Toki S.;
RT   "Purification, characterization and partial primary structure of morphine
RT   6-dehydrogenase from rabbit liver cytosol.";
RL   Arch. Biochem. Biophys. 341:81-88(1997).
CC   -!- FUNCTION: Catalyzes the dehydrogenation of morphine to morphinone. Uses
CC       both NAD and NADP, but the activity is much greater with NAD than with
CC       NADP.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=morphine + NAD(+) = H(+) + morphinone + NADH;
CC         Xref=Rhea:RHEA:14317, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57728, ChEBI:CHEBI:57945, ChEBI:CHEBI:58097;
CC         EC=1.1.1.218;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=morphine + NADP(+) = H(+) + morphinone + NADPH;
CC         Xref=Rhea:RHEA:14321, ChEBI:CHEBI:15378, ChEBI:CHEBI:57728,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58097, ChEBI:CHEBI:58349;
CC         EC=1.1.1.218;
CC   -!- ACTIVITY REGULATION: Strongly inhibited by sulfhydryl reagents and
CC       quercetin, but not by pyrazole, barbital and indomethacine.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 9.4 with NAD as the cofactor.;
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   PRIDE; P82810; -.
DR   eggNOG; KOG1577; Eukaryota.
DR   Proteomes; UP000001811; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050109; F:morphine 6-dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   SUPFAM; SSF51430; SSF51430; 1.
PE   1: Evidence at protein level;
KW   Alkaloid metabolism; Cytoplasm; Direct protein sequencing; NAD; NADP;
KW   Oxidoreductase; Reference proteome.
FT   CHAIN           <1..125
FT                   /note="Morphine 6-dehydrogenase"
FT                   /id="PRO_0000124645"
FT   BINDING         9..18
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000255"
FT   BINDING         74..111
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   NON_CONS        27..28
FT                   /evidence="ECO:0000305"
FT   NON_CONS        40..41
FT                   /evidence="ECO:0000305"
FT   NON_CONS        50..51
FT                   /evidence="ECO:0000305"
FT   NON_CONS        66..67
FT                   /evidence="ECO:0000305"
FT   NON_CONS        84..85
FT                   /evidence="ECO:0000305"
FT   NON_CONS        113..114
FT                   /evidence="ECO:0000305"
FT   NON_TER         1
SQ   SEQUENCE   125 AA;  14114 MW;  7C95292B1BEAECCB CRC64;
     HQRVTLNDGH SIPVLGFITY APDEVIKPGE EMFPTDEHGK SIGVSNFNHK RVXNQVEXHP
     YLNQSKDIVL VAYSALGSSR DPWKQSPALI ALRYQLQRGV VVLAKSFIER EIKVFGFQLS
     SEDMK