MOT10_RAT
ID MOT10_RAT Reviewed; 514 AA.
AC Q91Y77;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Monocarboxylate transporter 10;
DE Short=MCT 10;
DE AltName: Full=Aromatic amino acid transporter 1;
DE AltName: Full=Solute carrier family 16 member 10;
DE AltName: Full=T-type amino acid transporter 1;
GN Name=Slc16a10; Synonyms=Mct10, Tat1;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC STRAIN=Sprague-Dawley; TISSUE=Small intestine;
RX PubMed=11278508; DOI=10.1074/jbc.m009462200;
RA Kim D.K., Kanai Y., Chairoungdua A., Matsuo H., Cha S.H., Endou H.;
RT "Expression cloning of a Na+-independent aromatic amino acid transporter
RT with structural similarity to H+/monocarboxylate transporters.";
RL J. Biol. Chem. 276:17221-17228(2001).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262; SER-497 AND SER-503, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Sodium-independent transporter that mediates the uptake of
CC aromatic acids. Can function as a net efflux pathway for aromatic amino
CC acids in the basosolateral epithelial cells (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:11278508}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Basolateral cell membrane
CC {ECO:0000269|PubMed:11278508}. Note=In the epithelial cells is detected
CC in the basolateral membrane but not in the apical membrane.
CC -!- TISSUE SPECIFICITY: Strongly expressed in intestine, placenta and
CC liver. In small intestine is detected in the basolateral membrane (at
CC protein level). {ECO:0000269|PubMed:11278508}.
CC -!- PTM: Not N-glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Monocarboxylate porter (TC 2.A.1.13) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB047324; BAB55595.1; -; mRNA.
DR RefSeq; NP_620186.1; NM_138831.1.
DR AlphaFoldDB; Q91Y77; -.
DR SMR; Q91Y77; -.
DR STRING; 10116.ENSRNOP00000000718; -.
DR BindingDB; Q91Y77; -.
DR ChEMBL; CHEMBL2073717; -.
DR TCDB; 2.A.1.13.2; the major facilitator superfamily (mfs).
DR iPTMnet; Q91Y77; -.
DR PhosphoSitePlus; Q91Y77; -.
DR jPOST; Q91Y77; -.
DR PaxDb; Q91Y77; -.
DR PRIDE; Q91Y77; -.
DR GeneID; 170566; -.
DR KEGG; rno:170566; -.
DR UCSC; RGD:69197; rat.
DR CTD; 117247; -.
DR RGD; 69197; Slc16a10.
DR eggNOG; KOG2504; Eukaryota.
DR InParanoid; Q91Y77; -.
DR OrthoDB; 916876at2759; -.
DR PhylomeDB; Q91Y77; -.
DR Reactome; R-RNO-352230; Amino acid transport across the plasma membrane.
DR PRO; PR:Q91Y77; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0015173; F:aromatic amino acid transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015349; F:thyroid hormone transmembrane transporter activity; ISO:RGD.
DR GO; GO:0006590; P:thyroid hormone generation; ISO:RGD.
DR GO; GO:0070327; P:thyroid hormone transport; ISO:RGD.
DR GO; GO:0070460; P:thyroid-stimulating hormone secretion; ISO:RGD.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR030762; MCT10.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR PANTHER; PTHR11360:SF119; PTHR11360:SF119; 1.
DR Pfam; PF07690; MFS_1; 2.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..514
FT /note="Monocarboxylate transporter 10"
FT /id="PRO_0000314255"
FT TOPO_DOM 1..65
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 171..191
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 419..439
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..514
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..64
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 262
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 497
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 500
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8TF71"
FT MOD_RES 502
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3U9N9"
FT MOD_RES 503
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 514 AA; 55663 MW; 3AB082C0AE3B80A2 CRC64;
MVPSLEEPAA AERETNEAQP PGPAPSDDAP LPVPGPSDVS DGSVEKVEVE LTRSTGNQEP
PEPPEGGWGW LVMLAAMWCN GSVFGIQNAY GVLFVSMLET FGAKDDDNMA FKAAWVGSLS
MGMIFFCCPI VSVFTDMFGC RRTAVLGAAV GFVGLMSSSF VSSIEPLYFT YGVVFACGCS
FAYQPSLVIL GHYFKKRLGL VNGIVTAGSS VFTILLPLLL GNLTSTVGLC YTLRILCIFM
FVLFLAGFTY RPLVPSSKEK ESEDSRSSFF SRRKLSPPKK IFNFALFKET AYAVWAAGIP
LALFGYFVPY VHLMNHVKER FKDVNNKEVL FMCIGVTSGV GRLLFGRIAD YLPGVKKVYL
QVLSFFFIGL TSMMIPLCSV FGALIALCLI MGLFDGCFIS IMAPIAFELV GPQDASQAIG
FLLGFMSIPM TVGPPVAGLL HDKLGSYDLA FYLAGIPPFI GGAVLCLIPW IHSKKQREIS
KNTGGEKMEK MLANQSSLLS SSSGIFKKES DSII
