MOT1_ARATH
ID MOT1_ARATH Reviewed; 456 AA.
AC Q9SL95;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Molybdate transporter 1;
DE AltName: Full=Sulfate transporter like protein 5.2;
GN Name=MOT1; Synonyms=ST5.2, SULTR5.2; OrderedLocusNames=At2g25680;
GN ORFNames=F3N11.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia, and cv. Landsberg erecta;
RX PubMed=18003916; DOI=10.1073/pnas.0706373104;
RA Tomatsu H., Takano J., Takahashi H., Watanabe-Takahashi A., Shibagaki N.,
RA Fujiwara T.;
RT "An Arabidopsis thaliana high-affinity molybdate transporter required for
RT efficient uptake of molybdate from soil.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:18807-18812(2007).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=18454190; DOI=10.1371/journal.pgen.1000004;
RA Baxter I., Muthukumar B., Park H.C., Buchner P., Lahner B., Danku J.,
RA Zhao K., Lee J., Hawkesford M.J., Guerinot M.L., Salt D.E.;
RT "Variation in molybdenum content across broadly distributed populations of
RT Arabidopsis thaliana is controlled by a mitochondrial molybdenum
RT transporter (MOT1).";
RL PLoS Genet. 4:E1000004-E1000004(2008).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=21131548; DOI=10.1093/jxb/erq345;
RA Ide Y., Kusano M., Oikawa A., Fukushima A., Tomatsu H., Saito K.,
RA Hirai M.Y., Fujiwara T.;
RT "Effects of molybdenum deficiency and defects in molybdate transporter MOT1
RT on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis
RT thaliana.";
RL J. Exp. Bot. 62:1483-1497(2011).
RN [7]
RP FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=21815974; DOI=10.1111/j.1438-8677.2011.00448.x;
RA Gasber A., Klaumann S., Trentmann O., Trampczynska A., Clemens S.,
RA Schneider S., Sauer N., Feifer I., Bittner F., Mendel R.R., Neuhaus H.E.;
RT "Identification of an Arabidopsis solute carrier critical for intracellular
RT transport and inter-organ allocation of molybdate.";
RL Plant Biol. 13:710-718(2011).
CC -!- FUNCTION: High affinity molybdate transporter. Unable to transport
CC sulfate. {ECO:0000269|PubMed:18003916, ECO:0000269|PubMed:18454190,
CC ECO:0000269|PubMed:21131548, ECO:0000269|PubMed:21815974}.
CC -!- ACTIVITY REGULATION: Not inhibited by sulfate.
CC {ECO:0000269|PubMed:18003916}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=21 nM for molybdate {ECO:0000269|PubMed:18003916};
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Endomembrane system {ECO:0000305}; Multi-pass membrane protein
CC {ECO:0000305}. Mitochondrion membrane; Multi-pass membrane protein.
CC Note=(PubMed:18454190) shows a localization in the mitochondrial
CC membrane while (PubMed:18003916) shows a plasma membrane and
CC endomembrane system localization. {ECO:0000269|PubMed:18003916,
CC ECO:0000269|PubMed:18454190}.
CC -!- TISSUE SPECIFICITY: Strongly expressed in roots. Detected in the
CC vascular tissues of hypocotyls, in petioles and vascular tissues of
CC cotyledons and leaves, in mesophyll cells, stamen, sepals and siliques.
CC {ECO:0000269|PubMed:18003916, ECO:0000269|PubMed:18454190}.
CC -!- DEVELOPMENTAL STAGE: Not regulated during leaf senescence.
CC {ECO:0000269|PubMed:21815974}.
CC -!- DISRUPTION PHENOTYPE: 90% and 80% reduction in molybdate content in
CC shoots and roots respectively. Reduced growth of roots and shoots under
CC conditions of limited molybdate supply. 9-fold reduction in molybdate
CC content in leaf vacuoles. Decreased nitrate reductase activity due to a
CC reduced molybdate uptake. {ECO:0000269|PubMed:18003916,
CC ECO:0000269|PubMed:21131548, ECO:0000269|PubMed:21815974}.
CC -!- MISCELLANEOUS: The amino-acid substitution Asp-429 to Val observed in
CC cv. Landsberg erecta may decrease the molybdate transport activity. A
CC 54-bp deletion in the promoter of MOT1 is strongly associated with the
CC decreased expression of the gene and the resulting low molybdate
CC content observed in several cultivars.
CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC {ECO:0000305}.
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DR EMBL; AC006053; AAD31368.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07734.1; -; Genomic_DNA.
DR EMBL; AK221594; BAD95122.1; -; mRNA.
DR PIR; D84651; D84651.
DR RefSeq; NP_180139.1; NM_128127.4.
DR AlphaFoldDB; Q9SL95; -.
DR SMR; Q9SL95; -.
DR STRING; 3702.AT2G25680.1; -.
DR TCDB; 2.A.53.5.1; the sulfate permease (sulp) family.
DR PaxDb; Q9SL95; -.
DR PRIDE; Q9SL95; -.
DR ProteomicsDB; 250882; -.
DR EnsemblPlants; AT2G25680.1; AT2G25680.1; AT2G25680.
DR GeneID; 817109; -.
DR Gramene; AT2G25680.1; AT2G25680.1; AT2G25680.
DR KEGG; ath:AT2G25680; -.
DR Araport; AT2G25680; -.
DR TAIR; locus:2050261; AT2G25680.
DR eggNOG; ENOG502QRGR; Eukaryota.
DR HOGENOM; CLU_032158_0_1_1; -.
DR InParanoid; Q9SL95; -.
DR OMA; AGMLCHW; -.
DR OrthoDB; 684963at2759; -.
DR PhylomeDB; Q9SL95; -.
DR PRO; PR:Q9SL95; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SL95; baseline and differential.
DR Genevisible; Q9SL95; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0015098; F:molybdate ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015689; P:molybdate ion transport; IDA:TAIR.
DR InterPro; IPR031563; MOT1/MOT2.
DR PANTHER; PTHR31970; PTHR31970; 1.
DR Pfam; PF16983; MFS_MOT1; 2.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Mitochondrion; Molybdenum; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..456
FT /note="Molybdate transporter 1"
FT /id="PRO_0000417396"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 429
FT /note="D -> V (in strain: cv. Landsberg erecta)"
SQ SEQUENCE 456 AA; 48391 MW; A816D7C66B78FDD0 CRC64;
MESQSQRGQH ETPKRSRFTG MFHKLKTNLV FRSKLAEING AMGDLGTYIP IVLALTLAKD
LDLGTTLIFT GIYNAITGAV YGVPMPVQPM KSIAAVAISS TAEDFGIPEI MAAGICTGGI
LFVLGISGLM QLVFNIIPLS VVRGIQLSQG LAFAMSAVKY IRKEQNFSKS KSVGDRPWLG
LDGLVLALVC VLFIVLVNGD GEEEEEEEEG DGSRGRGRWG SVRKVIANVP SALLIFLLGV
VLAFIRKPSI VHDIKFGPSK MKIVRISRKA WRNGFLKGTV PQLPLSVLNS VVAVCKLSYD
LFPEKEFSAA SVSMTVGLMN MVGCWFGAMP TCHGAGGLAG QYKFGGRSGG CVALLGVAKL
VLGLVLGGSL VGILEKFPVG VLGALLLFAG VELAMAARDM NTKGDAFVML MCTSVSLGSN
AAIGFVAGDL LYVVLWMRNY GRAKPSSLPP QSGEHA
