MOT2_ARATH
ID MOT2_ARATH Reviewed; 464 AA.
AC Q0WP36; C0Z3C0; Q9C973;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2012, sequence version 2.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Molybdate transporter 2;
DE AltName: Full=Sulfate transporter like protein 5.1;
GN Name=MOT2; Synonyms=ST5.1, SULTR5.1; OrderedLocusNames=At1g80310;
GN ORFNames=F5I6.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=21131548; DOI=10.1093/jxb/erq345;
RA Ide Y., Kusano M., Oikawa A., Fukushima A., Tomatsu H., Saito K.,
RA Hirai M.Y., Fujiwara T.;
RT "Effects of molybdenum deficiency and defects in molybdate transporter MOT1
RT on transcript accumulation and nitrogen/sulphur metabolism in Arabidopsis
RT thaliana.";
RL J. Exp. Bot. 62:1483-1497(2011).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF 8-LEU-LEU-9, DISRUPTION
RP PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=21815974; DOI=10.1111/j.1438-8677.2011.00448.x;
RA Gasber A., Klaumann S., Trentmann O., Trampczynska A., Clemens S.,
RA Schneider S., Sauer N., Feifer I., Bittner F., Mendel R.R., Neuhaus H.E.;
RT "Identification of an Arabidopsis solute carrier critical for intracellular
RT transport and inter-organ allocation of molybdate.";
RL Plant Biol. 13:710-718(2011).
CC -!- FUNCTION: Molybdate transporter required for vacuolar molybdate export
CC during senescence. {ECO:0000269|PubMed:21131548,
CC ECO:0000269|PubMed:21815974}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:21815974};
CC Multi-pass membrane protein {ECO:0000269|PubMed:21815974}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q0WP36-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q0WP36-2; Sequence=VSP_043684;
CC -!- TISSUE SPECIFICITY: Expressed in leaves. Not detected in roots, shoots
CC and seeds.
CC -!- DEVELOPMENTAL STAGE: Up-regulated in senescing leaves.
CC {ECO:0000269|PubMed:21815974}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype and no effect on molybdate
CC content in shoots when grown in soil, but increased levels in leaves
CC and decreased levels in seeds. {ECO:0000269|PubMed:21131548,
CC ECO:0000269|PubMed:21815974}.
CC -!- MISCELLANEOUS: About 80% of the leaf molybdate is exported during
CC senescence.
CC -!- SIMILARITY: Belongs to the SLC26A/SulP transporter (TC 2.A.53) family.
CC {ECO:0000305}.
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DR EMBL; AC018848; AAG52436.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE36386.1; -; Genomic_DNA.
DR EMBL; AY064056; AAL36412.1; -; mRNA.
DR EMBL; BT008582; AAP40409.1; -; mRNA.
DR EMBL; AK229248; BAF01113.1; -; mRNA.
DR EMBL; AK319084; BAH57199.1; -; mRNA.
DR EMBL; AY084878; AAM61441.1; -; mRNA.
DR PIR; F96834; F96834.
DR RefSeq; NP_178147.1; NM_106680.3. [Q0WP36-1]
DR AlphaFoldDB; Q0WP36; -.
DR SMR; Q0WP36; -.
DR STRING; 3702.AT1G80310.1; -.
DR TCDB; 2.A.53.5.3; the sulfate permease (sulp) family.
DR SwissPalm; Q0WP36; -.
DR PaxDb; Q0WP36; -.
DR PRIDE; Q0WP36; -.
DR ProteomicsDB; 239063; -. [Q0WP36-1]
DR EnsemblPlants; AT1G80310.1; AT1G80310.1; AT1G80310. [Q0WP36-1]
DR GeneID; 844371; -.
DR Gramene; AT1G80310.1; AT1G80310.1; AT1G80310. [Q0WP36-1]
DR KEGG; ath:AT1G80310; -.
DR Araport; AT1G80310; -.
DR TAIR; locus:2034245; AT1G80310.
DR eggNOG; ENOG502QRGR; Eukaryota.
DR HOGENOM; CLU_032158_0_1_1; -.
DR InParanoid; Q0WP36; -.
DR OMA; DMNTKQE; -.
DR PhylomeDB; Q0WP36; -.
DR PRO; PR:Q0WP36; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q0WP36; differential.
DR Genevisible; Q0WP36; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0015098; F:molybdate ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:0090414; P:molybdate ion export from vacuole; IMP:TAIR.
DR GO; GO:0015689; P:molybdate ion transport; IMP:TAIR.
DR InterPro; IPR031563; MOT1/MOT2.
DR PANTHER; PTHR31970; PTHR31970; 1.
DR Pfam; PF16983; MFS_MOT1; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Membrane; Molybdenum; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..464
FT /note="Molybdate transporter 2"
FT /id="PRO_0000417397"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 116..136
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 8..9
FT /note="Tonoplast targeting signal"
FT VAR_SEQ 43..129
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_043684"
FT MUTAGEN 8..9
FT /note="LL->AA: Loss of vacuolar localization."
FT /evidence="ECO:0000269|PubMed:21815974"
FT CONFLICT 135
FT /note="V -> A (in Ref. 4; BAF01113)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 464 AA; 49551 MW; 7C8AC36E8580C312 CRC64;
METTTTPLLP GDRSRCGWLR RRLRLKNPLS SELSGAVGDL GTFIPIVLTL TLVSNLDLST
TLIFTGFYNI ATGLLFDIPM PVQPMKSIAA VAVSESPHLT PSQIAAAGAS TAATLLLLGA
TGAMSFLYNI IPLPVVRGVQ LSQGLQFAFT AIKYVRFNYD TATLKPSSSP RIWLGLDGLI
LALAALLFII LSTGSGNDRE AEDGDLAETS SNESQSRRRR LRLLSSIPSA LIVFALGLVL
CFIRDPSIFK DLKFGPSKFH ILRISWDDWK IGFLRAAIPQ IPLSVLNSVI AVCKLSNDLF
DKELSATTVS ISVGVMNLIG CWFGAMPVCH GAGGLAGQYR FGARSGLSVI FLGIGKLIVG
LVFGNSFVRI LSQFPIGILG VLLLFAGIEL AMASKDMNSK EDSFIMLVCA AVSMTGSSAA
LGFGCGVVLY LLLKLRTLDC SSVTLFSRSS DESQVDSEAA PRDV
