MOT5_MOUSE
ID MOT5_MOUSE Reviewed; 500 AA.
AC Q8R0M8; Q8BLA5; Q8BWE3; Q8R157;
DT 21-MAR-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Monocarboxylate transporter 5;
DE Short=MCT 5;
GN Name=Slc16a4; Synonyms=Mct5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J;
RC TISSUE=Corpora quadrigemina, Diencephalon, Hippocampus, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Proton-linked monocarboxylate transporter. Catalyzes the
CC rapid transport across the plasma membrane of many monocarboxylates
CC such as lactate, pyruvate, branched-chain oxo acids derived from
CC leucine, valine and isoleucine, and the ketone bodies acetoacetate,
CC beta-hydroxybutyrate and acetate (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8R0M8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8R0M8-2; Sequence=VSP_042511;
CC Name=3;
CC IsoId=Q8R0M8-3; Sequence=VSP_042510;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC Monocarboxylate porter (TC 2.A.1.13) family. {ECO:0000305}.
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DR EMBL; AK045797; BAC32495.1; -; mRNA.
DR EMBL; AK049873; BAC33967.1; -; mRNA.
DR EMBL; AK052779; BAC35143.1; -; mRNA.
DR EMBL; AK085492; BAC39456.1; -; mRNA.
DR EMBL; AK136837; BAE23142.1; -; mRNA.
DR EMBL; AC132405; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466607; EDL01900.1; -; Genomic_DNA.
DR EMBL; BC025441; AAH25441.1; -; mRNA.
DR EMBL; BC026596; AAH26596.1; -; mRNA.
DR CCDS; CCDS17736.1; -. [Q8R0M8-1]
DR CCDS; CCDS79997.1; -. [Q8R0M8-2]
DR RefSeq; NP_001297634.1; NM_001310705.1. [Q8R0M8-2]
DR RefSeq; NP_666248.1; NM_146136.1. [Q8R0M8-1]
DR AlphaFoldDB; Q8R0M8; -.
DR SMR; Q8R0M8; -.
DR STRING; 10090.ENSMUSP00000029502; -.
DR iPTMnet; Q8R0M8; -.
DR PhosphoSitePlus; Q8R0M8; -.
DR PaxDb; Q8R0M8; -.
DR PRIDE; Q8R0M8; -.
DR ProteomicsDB; 252597; -. [Q8R0M8-1]
DR ProteomicsDB; 252598; -. [Q8R0M8-2]
DR ProteomicsDB; 252599; -. [Q8R0M8-3]
DR Antibodypedia; 20096; 127 antibodies from 23 providers.
DR DNASU; 229699; -.
DR Ensembl; ENSMUST00000029502; ENSMUSP00000029502; ENSMUSG00000027896. [Q8R0M8-1]
DR Ensembl; ENSMUST00000106723; ENSMUSP00000102334; ENSMUSG00000027896. [Q8R0M8-2]
DR GeneID; 229699; -.
DR KEGG; mmu:229699; -.
DR UCSC; uc008qwx.1; mouse. [Q8R0M8-3]
DR UCSC; uc008qwy.2; mouse. [Q8R0M8-1]
DR CTD; 9122; -.
DR MGI; MGI:2385183; Slc16a4.
DR VEuPathDB; HostDB:ENSMUSG00000027896; -.
DR eggNOG; KOG2504; Eukaryota.
DR GeneTree; ENSGT00940000158411; -.
DR HOGENOM; CLU_001265_59_2_1; -.
DR InParanoid; Q8R0M8; -.
DR OMA; CKTNGCF; -.
DR OrthoDB; 916876at2759; -.
DR PhylomeDB; Q8R0M8; -.
DR TreeFam; TF313792; -.
DR BioGRID-ORCS; 229699; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Slc16a4; mouse.
DR PRO; PR:Q8R0M8; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q8R0M8; protein.
DR Bgee; ENSMUSG00000027896; Expressed in right kidney and 80 other tissues.
DR Genevisible; Q8R0M8; MM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0008028; F:monocarboxylic acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015718; P:monocarboxylic acid transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..500
FT /note="Monocarboxylate transporter 5"
FT /id="PRO_0000416125"
FT TOPO_DOM 1..16
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 38..58
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 88..108
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 109
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 130..153
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..195
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 196..304
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..342
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..374
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..430
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 452..463
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..484
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 485..500
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 350..500
FT /note="ITETVSQLISGWIADQNWIKKYQYHKSYLILCGVTNLLAPLATTFPLLMAYT
FT ILFAIFAGGYLALILPVLVDLSKNSRVHKFLGYASFFAGIAVLSGPPIAGWIYDYTQTY
FT TGSFYFSGTCYILSSVSLFFVPLAERWKRKQSDLLRTTIK -> KKNELSLIKVY (in
FT isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_042510"
FT VAR_SEQ 451..500
FT /note="GWIYDYTQTYTGSFYFSGTCYILSSVSLFFVPLAERWKRKQSDLLRTTIK
FT -> DVTHGWTTYLMHMKPLKT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_042511"
FT CONFLICT 366
FT /note="N -> D (in Ref. 1; BAC32495)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 500 AA; 55678 MW; 80B0FFF7D0C652E7 CRC64;
MVKKEKTPSP YTKPLDGGWG WMVVLHFFLV NVFVMGMTKT FAIFFVVFQE EFEGTSEQIG
WIGSIMSSLR FSAGPLAAII CDVLGEKATS ILGTFLVSGG YVISSWATGI PFLCVTMGLL
PGLGSAFLYQ VAAVVITKYF KKRLGLSTAI ARSGMGLTFL LAPFTKFLID LYDWTGALIL
FGAIILNLVP SSMLLRPIHS QSNNNSDIEN KGSILSATEP EASYKTETSK CKETQEPFIK
DSTMKKSEQP TTTLTVLGNQ SEEFSNRPHR NRPLLMSNGK SHKKKFVSWN CKQKLLDISL
FRNPFFYIFT WSFLLSQLAY FIPTFHLVAR AKTLGIDVMD ASYLVSVAGI TETVSQLISG
WIADQNWIKK YQYHKSYLIL CGVTNLLAPL ATTFPLLMAY TILFAIFAGG YLALILPVLV
DLSKNSRVHK FLGYASFFAG IAVLSGPPIA GWIYDYTQTY TGSFYFSGTC YILSSVSLFF
VPLAERWKRK QSDLLRTTIK
