ID   MOTA_BPT4               Reviewed;         211 AA.
AC   P22915;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   02-JUN-2021, entry version 108.
DE   RecName: Full=Middle transcription regulatory protein motA;
GN   Name=motA;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2287273; DOI=10.1111/j.1365-2958.1990.tb02059.x;
RA   Uzan M., Brody E., Favre R.;
RT   "Nucleotide sequence and control of transcription of the bacteriophage T4
RT   motA regulatory gene.";
RL   Mol. Microbiol. 4:1487-1496(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.19 ANGSTROMS) OF 2-96.
RX   PubMed=9155025; DOI=10.1093/emboj/16.8.1992;
RA   Finnin M.S., Cicero M.P., Davies C., Porter S.J., White S.W., Kreuzer K.N.;
RT   "The activation domain of the MotA transcription factor from bacteriophage
RT   T4.";
RL   EMBO J. 16:1992-2003(1997).
CC   -!- FUNCTION: Required for the transcriptional activation of middle
CC       promoters. Middle promoters are characterized by the presence of the
CC       conserved sequence [AT]3TGCTTNA (MotA box). MotA binds directly to MotA
CC       boxes.
CC   -!- MISCELLANEOUS: MotA synthesis starts immediately after infection and
CC       stops abruptly some 4-5 min later.
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DR   EMBL; Z48569; CAA88453.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42607.1; -; Genomic_DNA.
DR   PIR; JV0101; ZKBPT4.
DR   RefSeq; NP_049873.1; NC_000866.4.
DR   PDB; 1BJA; X-ray; 2.19 A; A/B=2-96.
DR   PDB; 1I1S; NMR; -; A=1-96.
DR   PDB; 1KAF; X-ray; 1.60 A; A/B/C/D/E/F=105-211.
DR   PDB; 5JLT; X-ray; 2.96 A; A/B/C/D=93-211.
DR   PDB; 6K4Y; EM; 3.79 A; M=1-211.
DR   PDBsum; 1BJA; -.
DR   PDBsum; 1I1S; -.
DR   PDBsum; 1KAF; -.
DR   PDBsum; 5JLT; -.
DR   PDBsum; 6K4Y; -.
DR   BMRB; P22915; -.
DR   SMR; P22915; -.
DR   GeneID; 1258740; -.
DR   KEGG; vg:1258740; -.
DR   EvolutionaryTrace; P22915; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.90.1150.20; -; 1.
DR   InterPro; IPR015241; MotA_Tscrpt_reg_C.
DR   InterPro; IPR036474; MotA_Tscrpt_reg_C_sf.
DR   InterPro; IPR015198; Phage_T4_MotA_Tscrpt_reg_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF09114; MotA_activ; 1.
DR   Pfam; PF09158; MotCF; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF69652; SSF69652; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..211
FT                   /note="Middle transcription regulatory protein motA"
FT                   /id="PRO_0000164956"
FT   DNA_BIND        23..42
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         140
FT                   /note="A->D: Temperature-sensitive."
FT   HELIX           4..10
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   TURN            11..13
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   HELIX           17..28
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   HELIX           34..39
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   STRAND          42..44
FT                   /evidence="ECO:0007829|PDB:1I1S"
FT   HELIX           46..57
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   TURN            58..60
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   STRAND          61..65
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   STRAND          68..71
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   HELIX           73..89
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   HELIX           91..94
FT                   /evidence="ECO:0007829|PDB:1BJA"
FT   HELIX           108..123
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   STRAND          128..134
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   STRAND          137..146
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   STRAND          149..154
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   STRAND          158..166
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   HELIX           169..176
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   TURN            177..179
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   STRAND          181..184
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   STRAND          188..195
FT                   /evidence="ECO:0007829|PDB:1KAF"
FT   HELIX           198..209
FT                   /evidence="ECO:0007829|PDB:1KAF"
SQ   SEQUENCE   211 AA;  23574 MW;  841625941A194E3F CRC64;
     MSKVTYIIKA SNDVLNEKTA TILITIAKKD FITAAEVREV HPDLGNAVVN SNIGVLIKKG
     LVEKSGDGLI ITGEAQDIIS NAATLYAQEN APELLKKRAT RKAREITSDM EEDKDLMLKL
     LDKNGFVLKK VEIYRSNYLA ILEKRTNGIR NFEINNNGNM RIFGYKMMEH HIQKFTDIGM
     SCKIAKNGNV YLDIKRSAEN IEAVITVASE L