ID   MOTI_PIG                Reviewed;         119 AA.
AC   P01307; A5X8W5; Q2I0F9;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 2.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Promotilin;
DE   Contains:
DE     RecName: Full=Motilin;
DE   Contains:
DE     RecName: Full=Motilin-associated peptide;
DE              Short=MAP;
DE   Flags: Precursor;
GN   Name=MLN;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=2456453; DOI=10.1210/mend-2-2-175;
RA   Bond C.T., Nilaver G., Godfrey B., Zimmerman E.A., Adelman J.P.;
RT   "Characterization of complementary deoxyribonucleic acid for precursor of
RT   porcine motilin.";
RL   Mol. Endocrinol. 2:175-180(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Ying M., Yang Z.;
RT   "Two SNP found in motilin in China Meishan pig.";
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Ying M., Yang Z.;
RT   "Sequencing and chromosome mapping of pig ghrelin and motilin genes.";
RL   Submitted (MAY-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   PROTEIN SEQUENCE OF 26-47.
RX   PubMed=4706833; DOI=10.1139/o73-066;
RA   Brown J.C., Cook M.A., Dryburgh J.R.;
RT   "Motilin, a gastric motor activity stimulating polypeptide: the complete
RT   amino acid sequence.";
RL   Can. J. Biochem. 51:533-537(1973).
RN   [5]
RP   SEQUENCE REVISION.
RX   PubMed=4856583; DOI=10.1139/o74-002;
RA   Schubert H., Brown J.C.;
RT   "Correction to the amino acid sequence of porcine motilin.";
RL   Can. J. Biochem. 52:7-8(1974).
RN   [6]
RP   SYNTHESIS.
RA   Yajima H., Kai Y., Kawatani H.;
RL   J. Chem. Soc. Chem. Commun. 1975:159-160(1975).
RN   [7]
RP   FUNCTION.
RX   PubMed=4941085; DOI=10.1139/y71-047;
RA   Brown J.C., Mutt V., Dryburgh J.R.;
RT   "The further purification of motilin, a gastric motor activity stimulating
RT   polypeptide from the mucosa of the small intestine of hogs.";
RL   Can. J. Physiol. Pharmacol. 49:399-405(1971).
RN   [8]
RP   STRUCTURE BY NMR OF 26-47.
RX   PubMed=2383556; DOI=10.1021/bi00476a015;
RA   Khan N., Graslund A., Ehrenberg A., Shriver J.;
RT   "Sequence-specific 1H NMR assignments and secondary structure of porcine
RT   motilin.";
RL   Biochemistry 29:5743-5751(1990).
RN   [9]
RP   STRUCTURE BY NMR OF 26-47.
RX   PubMed=1958665; DOI=10.1021/bi00111a012;
RA   Edmondson S., Khan N., Shriver J., Zdunek J., Graslund A.;
RT   "The solution structure of motilin from NMR distance constraints, distance
RT   geometry, molecular dynamics, and an iterative full relaxation matrix
RT   refinement.";
RL   Biochemistry 30:11271-11279(1991).
CC   -!- FUNCTION: Plays an important role in the regulation of interdigestive
CC       gastrointestinal motility and indirectly causes rhythmic contraction of
CC       duodenal and colonic smooth muscle. {ECO:0000269|PubMed:4941085}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the motilin family. {ECO:0000305}.
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DR   EMBL; M31219; AAA31088.1; -; mRNA.
DR   EMBL; DQ355970; ABC94729.1; -; mRNA.
DR   EMBL; DQ663494; ABG23101.1; -; Genomic_DNA.
DR   PIR; A40932; MSPG.
DR   RefSeq; NP_999400.1; NM_214235.1.
DR   RefSeq; XP_013833249.1; XM_013977795.1.
DR   RefSeq; XP_013833250.1; XM_013977796.1.
DR   AlphaFoldDB; P01307; -.
DR   BMRB; P01307; -.
DR   SMR; P01307; -.
DR   STRING; 9823.ENSSSCP00000001650; -.
DR   PaxDb; P01307; -.
DR   GeneID; 397466; -.
DR   KEGG; ssc:397466; -.
DR   CTD; 4295; -.
DR   eggNOG; ENOG502SS7F; Eukaryota.
DR   HOGENOM; CLU_154278_0_0_1; -.
DR   InParanoid; P01307; -.
DR   OrthoDB; 1512819at2759; -.
DR   TreeFam; TF336217; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   Genevisible; P01307; SS.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0031788; F:motilin receptor binding; IBA:GO_Central.
DR   InterPro; IPR006737; Motilin_assoc.
DR   InterPro; IPR006738; Motilin_ghrelin.
DR   InterPro; IPR015662; Promotilin.
DR   PANTHER; PTHR14156; PTHR14156; 1.
DR   Pfam; PF04643; Motilin_assoc; 1.
DR   Pfam; PF04644; Motilin_ghrelin; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing; Hormone;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000269|PubMed:4706833"
FT   CHAIN           26..119
FT                   /note="Promotilin"
FT                   /id="PRO_0000342173"
FT   PEPTIDE         26..47
FT                   /note="Motilin"
FT                   /id="PRO_0000019188"
FT   PEPTIDE         50..119
FT                   /note="Motilin-associated peptide"
FT                   /id="PRO_0000019189"
FT   REGION          40..72
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        29
FT                   /note="I -> S (in Ref. 1; AAA31088 and 3; ABG23101)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        103
FT                   /note="R -> G (in Ref. 2; ABC94729)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   119 AA;  13296 MW;  6E31A1038E142671 CRC64;
     MVSRKAVVVL LVVHAAAMLA SHTEAFVPIF TYGELQRMQE KERNKGQKKS LSVQQASEEL
     GPLDPSEPTK EEERVVIKLL APVDIGIRMD SRQLEKYRAT LERLLGQAPQ STQNQNAAK