ID   MP1_MNSV                Reviewed;          65 AA.
AC   Q89682; A0A0F7KKH0;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=Double gene block protein 1;
DE            Short=DGBp1;
DE   AltName: Full=Movement protein P7A;
GN   ORFNames=ORF2;
OS   Melon necrotic spot virus (MNSV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Procedovirinae; Gammacarmovirus.
OX   NCBI_TaxID=11987;
OH   NCBI_TaxID=3656; Cucumis melo (Muskmelon).
OH   NCBI_TaxID=3659; Cucumis sativus (Cucumber).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA], AND IDENTIFICATION.
RX   PubMed=2212985; DOI=10.1099/0022-1317-71-9-1887;
RA   Riviere C.J., Rochon D.M.;
RT   "Nucleotide sequence and genomic organization of melon necrotic spot
RT   virus.";
RL   J. Gen. Virol. 71:1887-1896(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Spain/SP-13/2001;
RA   Herrera-Vasquez J.A., Cordoba-Selles M.C., Cebrian M.C., Rossello J.A.,
RA   Alfaro-Fernandez A., Jorda C.;
RT   "Genetic diversity of Melon necrotic spot virus and Olpidium isolates from
RT   different origins.";
RL   Plant Pathol. 59:240-251(2010).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=ABCA13-01 {ECO:0000312|EMBL:AKH41058.1};
RX   PubMed=26184937; DOI=10.1128/genomea.00775-15;
RA   Li R., Zheng Y., Fei Z., Ling K.S.;
RT   "Complete Genome Sequence of an Emerging Melon Necrotic Spot Virus Isolate
RT   Infecting Greenhouse Cucumber in North America.";
RL   Genome Announc. 3:e00775-e00775(2015).
RN   [4]
RP   FUNCTION, MUTAGENESIS OF 13-ARG--ARG-19; 25-LYS--LYS-27; 32-LYS-LYS-33 AND
RP   LYS-43, AND SUBUNIT.
RX   PubMed=19796783; DOI=10.1016/j.virol.2009.08.042;
RA   Genoves A., Navarro J.A., Pallas V.;
RT   "A self-interacting carmovirus movement protein plays a role in binding of
RT   viral RNA during the cell-to-cell movement and shows an actin cytoskeleton
RT   dependent location in cell periphery.";
RL   Virology 395:133-142(2009).
CC   -!- FUNCTION: Cell-to-cell movement. Displays RNA-binding activity.
CC       {ECO:0000269|PubMed:19796783}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:19796783}.
CC   -!- SIMILARITY: Belongs to the carmovirus double gene block protein 1
CC       family. {ECO:0000305}.
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DR   EMBL; D12536; BAA02102.1; -; Genomic_RNA.
DR   EMBL; M29671; AAB02432.1; -; Genomic_RNA.
DR   EMBL; FJ621526; ACT53373.1; -; Genomic_RNA.
DR   EMBL; KR094068; AKH41058.1; -; Genomic_RNA.
DR   RefSeq; NP_041229.1; NC_001504.1.
DR   PRIDE; Q89682; -.
DR   GeneID; 1491978; -.
DR   KEGG; vg:1491978; -.
DR   Proteomes; UP000202003; Genome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046740; P:transport of virus in host, cell to cell; IEA:UniProtKB-KW.
DR   InterPro; IPR007982; Tombusvirus_movement.
DR   Pfam; PF05318; Tombus_movement; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; RNA-binding; Transport; Viral movement protein.
FT   CHAIN           1..65
FT                   /note="Double gene block protein 1"
FT                   /id="PRO_0000398307"
FT   REGION          1..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          17..43
FT                   /note="RNA-binding"
FT                   /evidence="ECO:0000305"
FT   COMPBIAS        11..26
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         13..19
FT                   /note="RGRSKER->AGASAEA: Complete loss of cell-to-cell
FT                   movement ability."
FT                   /evidence="ECO:0000269|PubMed:19796783"
FT   MUTAGEN         25..27
FT                   /note="KQK->AQA: Complete loss of cell-to-cell movement
FT                   ability."
FT                   /evidence="ECO:0000269|PubMed:19796783"
FT   MUTAGEN         32..33
FT                   /note="RK->AA: Complete loss of cell-to-cell movement
FT                   ability. Drastic loss of RNA-binding activity."
FT                   /evidence="ECO:0000269|PubMed:19796783"
FT   MUTAGEN         43
FT                   /note="K->A: 35% loss of cell-to-cell movement ability."
FT                   /evidence="ECO:0000269|PubMed:19796783"
SQ   SEQUENCE   65 AA;  7121 MW;  31030ADA243F318F CRC64;
     MDSQRTVELT NPRGRSKERG DSGGKQKNSM GRKIANDAIS ESKQGVMGAS TYIADKIKVT
     INFNF