MP63_MYCTU
ID MP63_MYCTU Reviewed; 159 AA.
AC P9WIP1; L0T8A1; O08224; P0A5Q2; P97175;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 35.
DE RecName: Full=Immunogenic protein MPT63;
DE AltName: Full=16 kDa immunoprotective extracellular protein;
DE AltName: Full=Antigen MPT63;
DE Flags: Precursor;
GN Name=mpt63; OrderedLocusNames=Rv1926c; ORFNames=MTCY09F9.38;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=8975887; DOI=10.1128/iai.65.1.16-23.1997;
RA Manca C.M.A., Lyashchenko K., Wiker H.G., Usai D., Colangeli R.,
RA Gennaro M.L.;
RT "Molecular cloning, purification, and serological characterization of
RT MPT63, a novel antigen secreted by Mycobacterium tuberculosis.";
RL Infect. Immun. 65:16-23(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=9169770; DOI=10.1128/iai.65.6.2321-2328.1997;
RA Harth G., Lee B.Y., Horwitz M.A.;
RT "High-level heterologous expression and secretion in rapidly growing
RT nonpathogenic mycobacteria of four major Mycobacterium tuberculosis
RT extracellular proteins considered to be leading vaccine candidates and drug
RT targets.";
RL Infect. Immun. 65:2321-2328(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [4]
RP PROTEIN SEQUENCE OF 30-49.
RX PubMed=7878014; DOI=10.1073/pnas.92.5.1530;
RA Horwitz M.A., Lee B.W., Dillon B.J., Harth G.;
RT "Protective immunity against tuberculosis induced by vaccination with major
RT extracellular proteins of Mycobacterium tuberculosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:1530-1534(1995).
RN [5]
RP PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
RX PubMed=1898899; DOI=10.1128/iai.59.1.372-382.1991;
RA Nagai S., Wiker H.G., Harboe M., Kinomoto M.;
RT "Isolation and partial characterization of major protein antigens in the
RT culture fluid of Mycobacterium tuberculosis.";
RL Infect. Immun. 59:372-382(1991).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- SUBCELLULAR LOCATION: Secreted.
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DR EMBL; U27119; AAB95083.1; -; Genomic_DNA.
DR EMBL; U82234; AAB61537.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP44693.1; -; Genomic_DNA.
DR PIR; B70635; B70635.
DR RefSeq; NP_216442.1; NC_000962.3.
DR RefSeq; WP_003409684.1; NZ_NVQJ01000034.1.
DR PDB; 1LMI; X-ray; 1.50 A; A=30-159.
DR PDBsum; 1LMI; -.
DR AlphaFoldDB; P9WIP1; -.
DR SMR; P9WIP1; -.
DR STRING; 83332.Rv1926c; -.
DR PaxDb; P9WIP1; -.
DR DNASU; 885334; -.
DR GeneID; 885334; -.
DR KEGG; mtu:Rv1926c; -.
DR TubercuList; Rv1926c; -.
DR eggNOG; ENOG5030I03; Bacteria.
DR OMA; VTMNNGM; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; IDA:MTBBASE.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR Gene3D; 2.60.40.1240; -; 1.
DR InterPro; IPR029050; Immunoprotect_excell_Ig-like.
DR InterPro; IPR015250; MPT63-like.
DR Pfam; PF09167; DUF1942; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:7878014"
FT CHAIN 30..159
FT /note="Immunogenic protein MPT63"
FT /id="PRO_0000021736"
FT STRAND 33..36
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 40..45
FT /evidence="ECO:0007829|PDB:1LMI"
FT TURN 46..49
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 50..61
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 73..87
FT /evidence="ECO:0007829|PDB:1LMI"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 96..99
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 105..108
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 128..137
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 144..148
FT /evidence="ECO:0007829|PDB:1LMI"
FT STRAND 150..158
FT /evidence="ECO:0007829|PDB:1LMI"
SQ SEQUENCE 159 AA; 16514 MW; EB563E1EAABCE428 CRC64;
MKLTTMIKTA VAVVAMAAIA TFAAPVALAA YPITGKLGSE LTMTDTVGQV VLGWKVSDLK
SSTAVIPGYP VAGQVWEATA TVNAIRGSVT PAVSQFNART ADGINYRVLW QAAGPDTISG
ATIPQGEQST GKIYFDVTGP SPTIVAMNNG MEDLLIWEP
