ID   MP64_MYCBO              Reviewed;         228 AA.
AC   P0A5Q5; A0A1R3Y097; P19996; X2BJ68;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 77.
DE   RecName: Full=Immunogenic protein MPB64;
DE   AltName: Full=Antigen MPB64;
DE   Flags: Precursor;
GN   Name=mpb64; OrderedLocusNames=BQ2027_MB2002C;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BCG;
RX   PubMed=2642468; DOI=10.1128/iai.57.1.283-288.1989;
RA   Yamaguchi R., Matsuo K., Yamazaki A., Abe C., Nagai S., Terasaka K.,
RA   Yamada T.;
RT   "Cloning and characterization of the gene for immunogenic protein MPB64 of
RT   Mycobacterium bovis BCG.";
RL   Infect. Immun. 57:283-288(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
RN   [4]
RP   PROTEIN SEQUENCE OF 24-53.
RX   PubMed=3514457; DOI=10.1128/iai.52.1.293-302.1986;
RA   Harboe M., Nagai S., Patarroyo M.E., Torres M., Ramirez C., Cruz N.;
RT   "Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis
RT   BCG.";
RL   Infect. Immun. 52:293-302(1986).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the RsiV family. {ECO:0000305}.
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DR   EMBL; U34849; AAC44034.1; -; Genomic_DNA.
DR   EMBL; LT708304; SIU00607.1; -; Genomic_DNA.
DR   PIR; A30545; A30545.
DR   RefSeq; NP_855652.1; NC_002945.3.
DR   RefSeq; WP_003409954.1; NC_002945.4.
DR   AlphaFoldDB; P0A5Q5; -.
DR   SMR; P0A5Q5; -.
DR   EnsemblBacteria; SIU00607; SIU00607; BQ2027_MB2002C.
DR   PATRIC; fig|233413.5.peg.2198; -.
DR   OMA; VIFFFDQ; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.90.640.20; -; 1.
DR   InterPro; IPR021729; DUF3298.
DR   InterPro; IPR037126; PdaC/RsiV-like_sf.
DR   Pfam; PF11738; DUF3298; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000269|PubMed:3514457"
FT   CHAIN           24..228
FT                   /note="Immunogenic protein MPB64"
FT                   /id="PRO_0000021737"
FT   CONFLICT        29
FT                   /note="C -> H (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        29
FT                   /note="C -> R (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        41
FT                   /note="C -> Y (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   228 AA;  24855 MW;  D79850CD1907999C CRC64;
     MRIKIFMLVT AVVLLCCSGV ATAAPKTYCE ELKGTDTGQA CQIQMSDPAY NINISLPSYY
     PDQKSLENYI AQTRDKFLSA ATSSTPREAP YELNITSATY QSAIPPRGTQ AVVLKVYQNA
     GGTHPTTTYK AFDWDQAYRK PITYDTLWQA DTDPLPVVFP IVQGELSKQT GQQVSIAPNA
     GLDPVNYQNF AVTNDGVIFF FNPGELLPEA AGPTQVLVPR SAIDSMLA