MP64_MYCTU
ID MP64_MYCTU Reviewed; 228 AA.
AC P9WIN9; L0TB55; P0A5Q4; P19996;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 34.
DE RecName: Full=Immunogenic protein MPT64;
DE AltName: Full=Antigen MPT64;
DE Flags: Precursor;
GN Name=mpt64; OrderedLocusNames=Rv1980c; ORFNames=MTCY39.39;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=H37Rv / 27294;
RX PubMed=7513311; DOI=10.1128/iai.62.5.2058-2064.1994;
RA Oettinger T., Andersen A.B.;
RT "Cloning and B-cell-epitope mapping of MPT64 from Mycobacterium
RT tuberculosis H37Rv.";
RL Infect. Immun. 62:2058-2064(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=9169770; DOI=10.1128/iai.65.6.2321-2328.1997;
RA Harth G., Lee B.Y., Horwitz M.A.;
RT "High-level heterologous expression and secretion in rapidly growing
RT nonpathogenic mycobacteria of four major Mycobacterium tuberculosis
RT extracellular proteins considered to be leading vaccine candidates and drug
RT targets.";
RL Infect. Immun. 65:2321-2328(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [4]
RP PROTEIN SEQUENCE OF 24-64; 89-119 AND 137-164.
RX PubMed=1502498; DOI=10.1111/j.1365-3083.1992.tb03104.x;
RA Wiker H.G., Nagai S., Harboe M., Ljungqvist L.;
RT "A family of cross-reacting proteins secreted by Mycobacterium
RT tuberculosis.";
RL Scand. J. Immunol. 36:307-319(1992).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- SIMILARITY: Belongs to the RsiV family. {ECO:0000305}.
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DR EMBL; X75361; CAA53143.1; -; mRNA.
DR EMBL; U82235; AAB61538.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP44749.1; -; Genomic_DNA.
DR PIR; B70756; B70756.
DR RefSeq; NP_216496.1; NC_000962.3.
DR RefSeq; WP_003409954.1; NZ_NVQJ01000048.1.
DR PDB; 2HHI; NMR; -; A=25-228.
DR PDBsum; 2HHI; -.
DR AlphaFoldDB; P9WIN9; -.
DR SMR; P9WIN9; -.
DR STRING; 83332.Rv1980c; -.
DR PaxDb; P9WIN9; -.
DR DNASU; 885925; -.
DR GeneID; 885925; -.
DR KEGG; mtu:Rv1980c; -.
DR TubercuList; Rv1980c; -.
DR eggNOG; ENOG502ZA8P; Bacteria.
DR OMA; VIFFFDQ; -.
DR PhylomeDB; P9WIN9; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; IDA:MTBBASE.
DR GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR GO; GO:0035375; F:zymogen binding; IPI:CAFA.
DR GO; GO:0009267; P:cellular response to starvation; IEP:MTBBASE.
DR Gene3D; 3.90.640.20; -; 1.
DR InterPro; IPR021729; DUF3298.
DR InterPro; IPR037126; PdaC/RsiV-like_sf.
DR Pfam; PF11738; DUF3298; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:1502498"
FT CHAIN 24..228
FT /note="Immunogenic protein MPT64"
FT /id="PRO_0000021738"
FT HELIX 28..31
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 41..47
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 50..55
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 61..63
FT /evidence="ECO:0007829|PDB:2HHI"
FT HELIX 64..81
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 92..101
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 109..120
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 121..123
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 127..135
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 136..139
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 144..146
FT /evidence="ECO:0007829|PDB:2HHI"
FT HELIX 159..169
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 179..181
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 185..187
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 191..193
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 196..201
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 204..207
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 209..211
FT /evidence="ECO:0007829|PDB:2HHI"
FT STRAND 214..218
FT /evidence="ECO:0007829|PDB:2HHI"
FT HELIX 220..223
FT /evidence="ECO:0007829|PDB:2HHI"
FT TURN 224..226
FT /evidence="ECO:0007829|PDB:2HHI"
SQ SEQUENCE 228 AA; 24855 MW; D79850CD1907999C CRC64;
MRIKIFMLVT AVVLLCCSGV ATAAPKTYCE ELKGTDTGQA CQIQMSDPAY NINISLPSYY
PDQKSLENYI AQTRDKFLSA ATSSTPREAP YELNITSATY QSAIPPRGTQ AVVLKVYQNA
GGTHPTTTYK AFDWDQAYRK PITYDTLWQA DTDPLPVVFP IVQGELSKQT GQQVSIAPNA
GLDPVNYQNF AVTNDGVIFF FNPGELLPEA AGPTQVLVPR SAIDSMLA
