ID   MP70_MYCBO              Reviewed;         193 AA.
AC   P0A669; A0A1R3Y2G8; Q48934; Q48946; Q48947; Q48948; Q50656; Q50769; X2BLT4;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Immunogenic protein MPB70;
DE   Flags: Precursor;
GN   Name=mpb70; OrderedLocusNames=BQ2027_MB2900;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=BCG / Pasteur, and BCG / Tokyo;
RX   PubMed=2663636; DOI=10.1016/0378-1097(89)90052-9;
RA   Terasaka K., Yamaguchi R., Matsuo K., Yamazaki A., Nagai S., Yamada T.;
RT   "Complete nucleotide sequence of immunogenic protein MPB70 from
RT   Mycobacterium bovis BCG.";
RL   FEMS Microbiol. Lett. 49:273-276(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BCG / Pasteur, and BCG / Tokyo;
RX   PubMed=1691265; DOI=10.1099/00221287-136-2-265;
RA   Radford A., Wood P., Billman-Jacobe H., Geysen H., Mason T., Tribbick G.;
RT   "Epitope mapping of the Mycobacterium bovis secretory protein MPB70 using
RT   overlapping peptide analysis.";
RL   J. Gen. Microbiol. 136:265-272(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BCG / Pasteur, and BCG / Tokyo;
RX   PubMed=7551028; DOI=10.1099/13500872-141-7-1601;
RA   Takemitsu M., Matsumoto S., Ohara N., Kitaura H., Mizuno A., Yamada T.;
RT   "Differential transcription of the MPB70 genes in two major groups of
RT   Mycobacterium bovis BCG substrains.";
RL   Microbiology 141:1601-1607(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-193.
RX   PubMed=3278986; DOI=10.1128/iai.56.4.921-925.1988;
RA   Radford A.J., Duffield B.J., Plackett P.;
RT   "Cloning of a species-specific antigen of Mycobacterium bovis.";
RL   Infect. Immun. 56:921-925(1988).
CC   -!- SUBUNIT: Generally found as a monomer; homodimer in culture fluids.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- MISCELLANEOUS: Produced in high concentration by BCG Tokyo, Moreau,
CC       Russia and Sweden (high-producer substrains), whereas in BCG Pasteur,
CC       Copenhagen and Tice (low-producer substrains) it is detected at 1%
CC       (w/w) or less of the concentration of BCG Tokyo. The difference in the
CC       secretion between BCG Tokyo and Pasteur was attributed to differential
CC       transcription efficiencies.
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DR   EMBL; M33916; AAA25366.1; -; Genomic_DNA.
DR   EMBL; D38229; BAA07402.1; -; Genomic_DNA.
DR   EMBL; D38230; BAA07403.1; -; Genomic_DNA.
DR   EMBL; LT708304; SIU01521.1; -; Genomic_DNA.
DR   EMBL; M37840; AAA25355.1; -; Genomic_DNA.
DR   PIR; A37195; A37195.
DR   PIR; A43502; A43502.
DR   PIR; A48320; A48320.
DR   RefSeq; NP_856545.1; NC_002945.3.
DR   RefSeq; WP_003414644.1; NC_002945.4.
DR   AlphaFoldDB; P0A669; -.
DR   SMR; P0A669; -.
DR   EnsemblBacteria; SIU01521; SIU01521; BQ2027_MB2900.
DR   GeneID; 45426864; -.
DR   PATRIC; fig|233413.5.peg.3183; -.
DR   OMA; RINNANV; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.30.180.10; -; 1.
DR   InterPro; IPR036378; FAS1_dom_sf.
DR   InterPro; IPR000782; FAS1_domain.
DR   Pfam; PF02469; Fasciclin; 1.
DR   SMART; SM00554; FAS1; 1.
DR   SUPFAM; SSF82153; SSF82153; 1.
DR   PROSITE; PS50213; FAS1; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..30
FT   CHAIN           31..193
FT                   /note="Immunogenic protein MPB70"
FT                   /id="PRO_0000008784"
FT   DOMAIN          57..189
FT                   /note="FAS1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00082"
FT   CONFLICT        16..17
FT                   /note="GL -> AV (in Ref. 3; AAA25366)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        101
FT                   /note="P -> R (in Ref. 6; AAA25355)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   193 AA;  19073 MW;  228695731C3FFB00 CRC64;
     MKVKNTIAAT SFAAAGLAAL AVAVSPPAAA GDLVGPGCAE YAAANPTGPA SVQGMSQDPV
     AVAASNNPEL TTLTAALSGQ LNPQVNLVDT LNSGQYTVFA PTNAAFSKLP ASTIDELKTN
     SSLLTSILTY HVVAGQTSPA NVVGTRQTLQ GASVTVTGQG NSLKVGNADV VCGGVSTANA
     TVYMIDSVLM PPA