ID   MP83_MYCBT              Reviewed;         220 AA.
AC   C1AFY9; P0A671; P71493; Q10790;
DT   07-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   25-MAY-2022, entry version 54.
DE   RecName: Full=Cell surface glycolipoprotein MPB83;
DE   AltName: Full=Lipoprotein p23;
DE   Flags: Precursor;
GN   Name=mpb83; OrderedLocusNames=JTY_2890;
OS   Mycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=561275;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, MUTAGENESIS OF CYS-25,
RP   DIACYLGLYCEROL AT CYS-25, AND PALMITOYLATION AT CYS-25.
RC   STRAIN=BCG / Tokyo 172 / ATCC 35737 / TMC 1019;
RX   PubMed=9099870; DOI=10.1016/s0378-1119(96)00806-2;
RA   Vosloo W., Tippoo P., Hughes E.J., Harriman N., Emms M., Beatty D.W.,
RA   Zappe H., Steyn L.M.;
RT   "Characterisation of a lipoprotein in Mycobacterium bovis (BCG) with
RT   sequence similarity to the secreted protein MPB70.";
RL   Gene 188:123-128(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BCG / Tokyo 172 / ATCC 35737 / TMC 1019;
RX   PubMed=19200449; DOI=10.1016/j.vaccine.2009.01.034;
RA   Seki M., Honda I., Fujita I., Yano I., Yamamoto S., Koyama A.;
RT   "Whole genome sequence analysis of Mycobacterium bovis bacillus Calmette-
RT   Guerin (BCG) Tokyo 172: a comparative study of BCG vaccine substrains.";
RL   Vaccine 27:1710-1716(2009).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303, ECO:0000305|PubMed:9099870}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303, ECO:0000305|PubMed:9099870}.
CC       Secreted, cell wall {ECO:0000250|UniProtKB:P9WNF3}. Secreted
CC       {ECO:0000250|UniProtKB:P0CAX7}.
CC   -!- PTM: O-glycosylated. Contains 0-3 mannose residues attached to residues
CC       48-49 in various configurations; the dominant glycoform is Thr-
CC       48(Man)/Thr-49(Man2) with an unusual Man(1->3)Man linkage, but
CC       Thr48(Man3)/Thr49(Man0) through to Thr48(Man0/)Thr49(Man3) are also
CC       seen (By similarity). {ECO:0000250|UniProtKB:P0CAX7}.
CC   -!- MISCELLANEOUS: Highly immunogenic.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB03901.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U28743; AAB03901.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP010918; BAH27168.1; -; Genomic_DNA.
DR   RefSeq; WP_003414630.1; NZ_CP014566.1.
DR   AlphaFoldDB; C1AFY9; -.
DR   SMR; C1AFY9; -.
DR   KEGG; mbt:JTY_2890; -.
DR   HOGENOM; CLU_031281_3_0_11; -.
DR   OMA; ICGGVQT; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.30.180.10; -; 1.
DR   InterPro; IPR036378; FAS1_dom_sf.
DR   InterPro; IPR000782; FAS1_domain.
DR   Pfam; PF02469; Fasciclin; 1.
DR   SMART; SM00554; FAS1; 1.
DR   SUPFAM; SSF82153; SSF82153; 1.
DR   PROSITE; PS50213; FAS1; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Cell wall; Glycoprotein; Lipoprotein; Membrane; Palmitate;
KW   Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000305|PubMed:9099870"
FT   CHAIN           25..220
FT                   /note="Cell surface glycolipoprotein MPB83"
FT                   /id="PRO_0000379526"
FT   DOMAIN          83..215
FT                   /note="FAS1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00082"
FT   LIPID           25
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305|PubMed:9099870"
FT   LIPID           25
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305|PubMed:9099870"
FT   CARBOHYD        48
FT                   /note="O-linked (Man...) threonine"
FT                   /evidence="ECO:0000250|UniProtKB:P0CAX7"
FT   CARBOHYD        49
FT                   /note="O-linked (Man...) threonine"
FT                   /evidence="ECO:0000250|UniProtKB:P0CAX7"
FT   MUTAGEN         25
FT                   /note="C->S: Loss of membrane localization."
FT                   /evidence="ECO:0000269|PubMed:9099870"
SQ   SEQUENCE   220 AA;  22070 MW;  5CB99A4B51852A98 CRC64;
     MINVQAKPAA AASLAAIAIA FLAGCSSTKP VSQDTSPKPA TSPAAPVTTA AMADPAADLI
     GRGCAQYAAQ NPTGPGSVAG MAQDPVATAA SNNPMLSTLT SALSGKLNPD VNLVDTLNGG
     EYTVFAPTNA AFDKLPAATI DQLKTDAKLL SSILTYHVIA GQASPSRIDG THQTLQGADL
     TVIGARDDLM VNNAGLVCGG VHTANATVYM IDTVLMPPAQ