ID   ARNE_ECO24              Reviewed;         111 AA.
AC   A7ZP76;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE            Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE   AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN   Name=arnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN   OrderedLocusNames=EcE24377A_2553;
OS   Escherichia coli O139:H28 (strain E24377A / ETEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331111;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=E24377A / ETEC;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
CC   -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC       (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC       periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01869}.
CC   -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01869}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SIMILARITY: Belongs to the ArnE family. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
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DR   EMBL; CP000800; ABV16768.1; -; Genomic_DNA.
DR   RefSeq; WP_000638031.1; NC_009801.1.
DR   AlphaFoldDB; A7ZP76; -.
DR   SMR; A7ZP76; -.
DR   EnsemblBacteria; ABV16768; ABV16768; EcE24377A_2553.
DR   GeneID; 66673856; -.
DR   KEGG; ecw:EcE24377A_2553; -.
DR   HOGENOM; CLU_131462_5_1_6; -.
DR   OMA; TCAGQLC; -.
DR   UniPathway; UPA00030; -.
DR   Proteomes; UP000001122; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_01869; Flippase_ArnE; 1.
DR   InterPro; IPR000620; EamA_dom.
DR   InterPro; IPR022883; Flippase_ArnE.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00892; EamA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..111
FT                   /note="Probable 4-amino-4-deoxy-L-arabinose-
FT                   phosphoundecaprenol flippase subunit ArnE"
FT                   /id="PRO_0000382963"
FT   TOPO_DOM        1..35
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TOPO_DOM        57..60
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TOPO_DOM        82..87
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TOPO_DOM        109..111
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          40..109
FT                   /note="EamA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
SQ   SEQUENCE   111 AA;  12192 MW;  7CFA06D75DA33D69 CRC64;
     MIWLTLVFAS LLSVAGQLCQ KQATCFVAIN KRRKHIVLWL GLALACLGLA MVLWLLVLQN
     VPVGIAYPML SLNFVWVTLA AVKLWHEPVS PRHWCGVAFI IGGIVILGST V