MPAL1_LOLPR
ID MPAL1_LOLPR Reviewed; 263 AA.
AC P14946; P19964;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1991, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Pollen allergen Lol p 1;
DE AltName: Full=Allergen Lol p I;
DE AltName: Full=Allergen R7;
DE AltName: Allergen=Lol p 1;
DE Flags: Precursor;
OS Lolium perenne (Perennial ryegrass).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Poodae; Poeae; Poeae Chloroplast Group 2 (Poeae type); Loliinae;
OC Lolium.
OX NCBI_TaxID=4522;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1697854; DOI=10.1016/s0021-9258(17)46209-0;
RA Perez M., Ishioka G.Y., Walker L.E., Chesnut R.W.;
RT "cDNA cloning and immunological characterization of the rye grass allergen
RT Lol p I.";
RL J. Biol. Chem. 265:16210-16215(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 24-48.
RC TISSUE=Pollen;
RX PubMed=2001733; DOI=10.1016/0014-5793(91)80151-r;
RA Griffith I.J., Smith P.M., Pollock J., Theerakulpisut P., Avjoglu A.,
RA Davies S., Hough T., Singh M.B., Simpson R.J., Ward L.D., Knox R.B.;
RT "Cloning and sequencing of Lol pI, the major allergenic protein of rye-
RT grass pollen.";
RL FEBS Lett. 279:210-215(1991).
RN [3]
RP PROTEIN SEQUENCE OF 24-53.
RC TISSUE=Pollen;
RX PubMed=3718469; DOI=10.1042/bj2340305;
RA Cottam G.P., Moran D.M., Standring R.;
RT "Physicochemical and immunochemical characterization of allergenic proteins
RT from rye-grass (Lolium perenne) pollen prepared by a rapid and efficient
RT purification method.";
RL Biochem. J. 234:305-310(1986).
RN [4]
RP PROTEIN SEQUENCE OF 236-263.
RX PubMed=2475768; DOI=10.1016/0161-5890(89)90007-2;
RA Esch R.E., Klapper D.G.;
RT "Isolation and characterization of a major cross-reactive grass group I
RT allergenic determinant.";
RL Mol. Immunol. 26:557-561(1989).
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALLERGEN: Causes an allergic reaction in human. Causes grass pollen
CC allergy. Binds to IgE.
CC -!- SIMILARITY: Belongs to the expansin family. Expansin B subfamily.
CC {ECO:0000305}.
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DR EMBL; M57474; AAA63279.1; -; mRNA.
DR EMBL; M57476; AAA63278.1; -; mRNA.
DR PIR; A23341; A23341.
DR PIR; B37881; B37881.
DR AlphaFoldDB; P14946; -.
DR SMR; P14946; -.
DR Allergome; 450; Lol p 1.
DR Allergome; 451; Lol p 1.0101.
DR Allergome; 452; Lol p 1.0102.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IEA:UniProt.
DR GO; GO:0019953; P:sexual reproduction; IEA:InterPro.
DR Gene3D; 2.40.40.10; -; 1.
DR Gene3D; 2.60.40.760; -; 1.
DR InterPro; IPR007118; Expan_Lol_pI.
DR InterPro; IPR007112; Expansin/allergen_DPBB_dom.
DR InterPro; IPR007117; Expansin_CBD.
DR InterPro; IPR036749; Expansin_CBD_sf.
DR InterPro; IPR005795; LolPI.
DR InterPro; IPR009009; RlpA-like_DPBB.
DR InterPro; IPR036908; RlpA-like_sf.
DR Pfam; PF03330; DPBB_1; 1.
DR Pfam; PF01357; Expansin_C; 1.
DR PRINTS; PR01225; EXPANSNFAMLY.
DR PRINTS; PR00829; LOLP1ALLERGN.
DR SMART; SM00837; DPBB_1; 1.
DR SUPFAM; SSF49590; SSF49590; 1.
DR SUPFAM; SSF50685; SSF50685; 1.
DR PROSITE; PS50843; EXPANSIN_CBD; 1.
DR PROSITE; PS50842; EXPANSIN_EG45; 1.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Glycoprotein; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:2001733,
FT ECO:0000269|PubMed:3718469"
FT CHAIN 24..263
FT /note="Pollen allergen Lol p 1"
FT /id="PRO_0000008720"
FT DOMAIN 61..167
FT /note="Expansin-like EG45"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00079"
FT DOMAIN 181..262
FT /note="Expansin-like CBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 68
FT /note="N -> D"
FT VARIANT 177
FT /note="A -> G"
FT VARIANT 210
FT /note="I -> T"
FT VARIANT 246
FT /note="F -> V"
FT CONFLICT 28
FT /note="P -> C (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 31
FT /note="P -> C (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 48
FT /note="W -> WW (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 28438 MW; 7675896F279C88C9 CRC64;
MASSSSVLLV VALFAVFLGS AHGIAKVPPG PNITAEYGDK WLDAKSTWYG KPTGAGPKDN
GGACGYKNVD KAPFNGMTGC GNTPIFKDGR GCGSCFEIKC TKPESCSGEA VTVTITDDNE
EPIAPYHFDL SGHAFGSMAK KGEEQNVRSA GELELQFRRV KCKYPDDTKP TFHVEKASNP
NYLAILVKYV DGDGDVVAVD IKEKGKDKWI ELKESWGAVW RIDTPDKLTG PFTVRYTTEG
GTKSEFEDVI PEGWKADTSY SAK
