ID   MPAO_CLAUC              Reviewed;         445 AA.
AC   A0A0R8YXT5;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   17-FEB-2016, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Methylphloroacetophenone oxidase {ECO:0000303|PubMed:26895859};
DE            Short=MPAO {ECO:0000303|PubMed:26895859};
DE            EC=1.-.-.- {ECO:0000305};
DE   AltName: Full=Cytochrome P450 monooxygenase MPAO {ECO:0000303|PubMed:26895859};
DE   AltName: Full=Usnic acid biosynthesis protein MPAO {ECO:0000305};
GN   Name=MPAO {ECO:0000303|PubMed:26895859};
OS   Cladonia uncialis (Cup lichen).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Lecanoromycetes;
OC   OSLEUM clade; Lecanoromycetidae; Lecanorales; Lecanorineae; Cladoniaceae;
OC   Cladonia.
OX   NCBI_TaxID=174080;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RX   PubMed=26895859; DOI=10.1016/j.funbio.2015.10.009;
RA   Abdel-Hameed M.D., Bertrand R.L., Piercey-Normore M.D., Sorensen J.L.;
RT   "Putative identification of the usnic acid biosynthetic gene cluster by de
RT   novo whole-genome sequencing of a lichen-forming fungus.";
RL   Fungal Biol. 120:306-316(2016).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Wen L., He K., Yang H.;
RL   Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   REVIEW ON BIOTECHNOLOGY.
RX   PubMed=25707417; DOI=10.1080/14786419.2015.1007455;
RA   Araujo A.A., de Melo M.G., Rabelo T.K., Nunes P.S., Santos S.L.,
RA   Serafini M.R., Santos M.R., Quintans-Junior L.J., Gelain D.P.;
RT   "Review of the biological properties and toxicity of usnic acid.";
RL   Nat. Prod. Res. 29:2167-2180(2015).
CC   -!- FUNCTION: Methylphloroacetophenone oxidase; part of the gene cluster
CC       that mediates the biosynthesis of usnic acid, a dibenzofuran lichen
CC       product possessing a broad spectrum of biological activities
CC       (PubMed:26895859). Two genes, mpas and mpao, comprise the usnic acid
CC       biosynthetic gene cluster with a single post-PKS enzyme, the
CC       methylphloracetophenone oxidase (mpao) (PubMed:26895859). The
CC       methylphloroacetophenone synthase (mpas) is a non-reducing polyketide
CC       synthase that produces methylphloracetophenone from acetate via a
CC       methylated tetraketide intermediate (PubMed:26895859). The
CC       methylphloroacetophenone oxidase then carries out the oxidative
CC       dimerization of methylphloracetophenone to usnic acid
CC       (PubMed:26895859). {ECO:0000269|PubMed:26895859}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:26895859}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- BIOTECHNOLOGY: Lichens belonging to usnic acid-containing genera have
CC       been used as crude drugs throughout the world (PubMed:25707417). Usnic
CC       acid is a potentially interesting candidate for such activities as
CC       anti-inflammatory, analgesic, healing, antioxidant, antimicrobial,
CC       antiprotozoal, antiviral, larvicidal and UV protection
CC       (PubMed:25707417). However, it was shown to be toxic for the liver and
CC       to lead to contact allergy (PubMed:25707417).
CC       {ECO:0000269|PubMed:25707417}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KT363733; ALA62324.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0R8YXT5; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   SUPFAM; SSF48264; SSF48264; 1.
PE   1: Evidence at protein level;
KW   Glycoprotein; Membrane; Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..445
FT                   /note="Methylphloroacetophenone oxidase"
FT                   /id="PRO_0000437592"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        51
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   445 AA;  49443 MW;  7A8E55718FDC0A77 CRC64;
     MISPVSSILA SIWDNSKLLL DHTSVLSIAL IGVACAISIR SILYVRLACA NYSILLLTHA
     QRLRLAYSTP LRHVPGPWYA KFTALGLRAN DVAGNRWYYV QGLHKKYGSI VRIAPEEVAI
     SDPKVVSKVH ALGTEFRKRQ QPGTPFNIFS ISDPKAHRTR QRFYAKAFSD ETLKASTEPA
     VRQLIKTAVA SIKRDAALRK DHTADVYKWC MLFGSDVAFQ VIYGNSNTEG LMATQKTTDE
     VIMGAYLQRM NAWAQFCFPV FLLGRWLSPL SPTLHNIFRV EEKYGDFWQE GQRQREIAAR
     TVFVQNTKYS KNDGVFSVSD EVKLSDVDIA HDITTFLGAG GEPVGASLVF LIWQVLRMPD
     LQRELEAEVA GLTEPITDAT TAQLPILNGV IYETLRLYGG GVTQMPRYAP IATELGGYVI
     PPGTAVTTHT GALHRNPAAW DDPEK