ID   MPAP2_PHLPR             Reviewed;         122 AA.
AC   P43214;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Pollen allergen Phl p 2;
DE   AltName: Full=Allergen Phl p II;
DE   AltName: Allergen=Phl p 2;
DE   Flags: Precursor;
GN   Name=PHLPII;
OS   Phleum pratense (Common timothy).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Poodae; Poeae; Poeae Chloroplast Group 2 (Poeae type); Poinae;
OC   Phleum.
OX   NCBI_TaxID=15957;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND ALLERGEN.
RC   TISSUE=Pollen;
RX   PubMed=8262175; DOI=10.1016/0014-5793(93)80406-k;
RA   Dolecek C., Vrtala S., Laffer S., Steinberger P., Kraft D., Scheiner O.,
RA   Valenta R.;
RT   "Molecular characterization of Phl p II, a major timothy grass (Phleum
RT   pratense) pollen allergen.";
RL   FEBS Lett. 335:299-304(1993).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
RX   PubMed=9130496; DOI=10.1159/000237520;
RA   Fedorov A.A., Ball T., Valenta R., Almo S.C.;
RT   "X-ray crystal structures of birch pollen profilin and Phl p 2.";
RL   Int. Arch. Allergy Immunol. 113:109-113(1997).
RN   [3]
RP   STRUCTURE BY NMR.
RX   PubMed=10467147; DOI=10.1016/s0969-2126(99)80121-x;
RA   De Marino S., Morelli M.A.C., Fraternali F., Tamborini E., Musco G.,
RA   Vrtala S., Dolecek C., Arosio P., Valenta R., Pastore A.;
RT   "An immunoglobulin-like fold in a major plant allergen: the solution
RT   structure of Phl p 2 from timothy grass pollen.";
RL   Structure 7:943-952(1999).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Pollen specific.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Causes grass pollen
CC       allergy. Binds to IgE. {ECO:0000269|PubMed:8262175}.
CC   -!- SIMILARITY: Belongs to the expansin family. Expansin B subfamily.
CC       {ECO:0000305}.
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DR   EMBL; X75925; CAA53529.1; -; mRNA.
DR   PIR; S39457; S39457.
DR   PDB; 1BMW; NMR; -; A=27-122.
DR   PDB; 1WHO; X-ray; 1.90 A; A=27-122.
DR   PDB; 1WHP; X-ray; 3.00 A; A=27-122.
DR   PDB; 2VXQ; X-ray; 1.90 A; A=27-122.
DR   PDBsum; 1BMW; -.
DR   PDBsum; 1WHO; -.
DR   PDBsum; 1WHP; -.
DR   PDBsum; 2VXQ; -.
DR   AlphaFoldDB; P43214; -.
DR   SMR; P43214; -.
DR   Allergome; 3419; Phl p 2.0101.
DR   Allergome; 555; Phl p 2.
DR   ABCD; P43214; 7 sequenced antibodies.
DR   EvolutionaryTrace; P43214; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.60.40.760; -; 1.
DR   InterPro; IPR005453; Allergen_Lolp2.
DR   InterPro; IPR007117; Expansin_CBD.
DR   InterPro; IPR036749; Expansin_CBD_sf.
DR   Pfam; PF01357; Expansin_C; 1.
DR   PRINTS; PR01637; LOLP2ALLERGN.
DR   SUPFAM; SSF49590; SSF49590; 1.
DR   PROSITE; PS50843; EXPANSIN_CBD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Allergen; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..122
FT                   /note="Pollen allergen Phl p 2"
FT                   /id="PRO_0000008723"
FT   DOMAIN          41..120
FT                   /note="Expansin-like CBD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00078"
FT   STRAND          29..34
FT                   /evidence="ECO:0007829|PDB:1WHO"
FT   STRAND          42..49
FT                   /evidence="ECO:0007829|PDB:1WHO"
FT   STRAND          50..52
FT                   /evidence="ECO:0007829|PDB:1BMW"
FT   STRAND          54..60
FT                   /evidence="ECO:0007829|PDB:1WHO"
FT   STRAND          62..66
FT                   /evidence="ECO:0007829|PDB:1BMW"
FT   HELIX           74..76
FT                   /evidence="ECO:0007829|PDB:2VXQ"
FT   STRAND          77..81
FT                   /evidence="ECO:0007829|PDB:1WHO"
FT   STRAND          88..96
FT                   /evidence="ECO:0007829|PDB:1WHO"
FT   TURN            97..99
FT                   /evidence="ECO:0007829|PDB:1BMW"
FT   STRAND          101..108
FT                   /evidence="ECO:0007829|PDB:1WHO"
FT   STRAND          114..116
FT                   /evidence="ECO:0007829|PDB:1BMW"
SQ   SEQUENCE   122 AA;  13362 MW;  708B911B25EE26F4 CRC64;
     MSMASSSSSS LLAMAVLAAL FAGAWCVPKV TFTVEKGSNE KHLAVLVKYE GDTMAEVELR
     EHGSDEWVAM TKGEGGVWTF DSEEPLQGPF NFRFLTEKGM KNVFDDVVPE KYTIGATYAP
     EE