ID   ARNE_ECO45              Reviewed;         111 AA.
AC   B7MG25;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Probable 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE            Short=L-Ara4N-phosphoundecaprenol flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
DE   AltName: Full=Undecaprenyl phosphate-aminoarabinose flippase subunit ArnE {ECO:0000255|HAMAP-Rule:MF_01869};
GN   Name=arnE {ECO:0000255|HAMAP-Rule:MF_01869}; OrderedLocusNames=ECS88_2408;
OS   Escherichia coli O45:K1 (strain S88 / ExPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=585035;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=S88 / ExPEC;
RX   PubMed=19165319; DOI=10.1371/journal.pgen.1000344;
RA   Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P.,
RA   Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H.,
RA   Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E.,
RA   Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C.,
RA   Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S.,
RA   Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J.,
RA   Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E.;
RT   "Organised genome dynamics in the Escherichia coli species results in
RT   highly diverse adaptive paths.";
RL   PLoS Genet. 5:E1000344-E1000344(2009).
CC   -!- FUNCTION: Translocates 4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
CC       (alpha-L-Ara4N-phosphoundecaprenol) from the cytoplasmic to the
CC       periplasmic side of the inner membrane. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; lipopolysaccharide
CC       biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01869}.
CC   -!- SUBUNIT: Heterodimer of ArnE and ArnF. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01869}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
CC   -!- SIMILARITY: Belongs to the ArnE family. {ECO:0000255|HAMAP-
CC       Rule:MF_01869}.
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DR   EMBL; CU928161; CAR03687.1; -; Genomic_DNA.
DR   RefSeq; WP_000638016.1; NC_011742.1.
DR   AlphaFoldDB; B7MG25; -.
DR   EnsemblBacteria; CAR03687; CAR03687; ECS88_2408.
DR   KEGG; ecz:ECS88_2408; -.
DR   HOGENOM; CLU_131462_5_1_6; -.
DR   OMA; TCAGQLC; -.
DR   UniPathway; UPA00030; -.
DR   Proteomes; UP000000747; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:1901505; F:carbohydrate derivative transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR   HAMAP; MF_01869; Flippase_ArnE; 1.
DR   InterPro; IPR000620; EamA_dom.
DR   InterPro; IPR022883; Flippase_ArnE.
DR   InterPro; IPR000390; Small_drug/metabolite_transptr.
DR   PANTHER; PTHR30561; PTHR30561; 1.
DR   Pfam; PF00892; EamA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Lipid A biosynthesis;
KW   Lipid biosynthesis; Lipid metabolism; Lipopolysaccharide biosynthesis;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..111
FT                   /note="Probable 4-amino-4-deoxy-L-arabinose-
FT                   phosphoundecaprenol flippase subunit ArnE"
FT                   /id="PRO_0000382968"
FT   TOPO_DOM        1..35
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TOPO_DOM        57..60
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TOPO_DOM        82..87
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
FT   TOPO_DOM        109..111
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          40..109
FT                   /note="EamA"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01869"
SQ   SEQUENCE   111 AA;  12166 MW;  34EC77ADEDCBE09D CRC64;
     MIWLTLVFAS LLSVAGQLCQ KQATCFAAVN KRRKHIVLWL GLALACLGLA MVLWLLVLQN
     VPVGIAYPML SLNFVWVTLA AVKLWHEPVS LRHWCGVAFI IGGIVILGST V