MPC1_YEAST
ID MPC1_YEAST Reviewed; 130 AA.
AC P53157; D6VU64;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Mitochondrial pyruvate carrier 1 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
DE Short=MPC1 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
DE AltName: Full=Protein FMP37 {ECO:0000303|PubMed:14576278};
GN Name=MPC1 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
GN Synonyms=FMP37 {ECO:0000303|PubMed:14576278};
GN OrderedLocusNames=YGL080W {ECO:0000312|SGD:S000003048};
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9290212;
RX DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT chromosome VII.";
RL Yeast 13:1077-1090(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=14576278; DOI=10.1073/pnas.2135385100;
RA Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E.,
RA Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P.,
RA Pfanner N., Meisinger C.;
RT "The proteome of Saccharomyces cerevisiae mitochondria.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, AND DISRUPTION PHENOTYPE.
RX PubMed=22628554; DOI=10.1126/science.1218530;
RA Herzig S., Raemy E., Montessuit S., Veuthey J.L., Zamboni N.,
RA Westermann B., Kunji E.R., Martinou J.C.;
RT "Identification and functional expression of the mitochondrial pyruvate
RT carrier.";
RL Science 337:93-96(2012).
RN [9]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=22628558; DOI=10.1126/science.1218099;
RA Bricker D.K., Taylor E.B., Schell J.C., Orsak T., Boutron A., Chen Y.C.,
RA Cox J.E., Cardon C.M., Van Vranken J.G., Dephoure N., Redin C., Boudina S.,
RA Gygi S.P., Brivet M., Thummel C.S., Rutter J.;
RT "A mitochondrial pyruvate carrier required for pyruvate uptake in yeast,
RT Drosophila, and humans.";
RL Science 337:96-100(2012).
CC -!- FUNCTION: Mediates the uptake of pyruvate into mitochondria.
CC {ECO:0000269|PubMed:22628554, ECO:0000269|PubMed:22628558}.
CC -!- SUBUNIT: The functional 150 kDa pyruvate import complex is a heteromer
CC of MPC1 and either MPC2 or MPC3. {ECO:0000269|PubMed:22628554,
CC ECO:0000269|PubMed:22628558}.
CC -!- INTERACTION:
CC P53157; P38857: MPC2; NbExp=9; IntAct=EBI-23845, EBI-24818;
CC P53157; P53311: MPC3; NbExp=4; IntAct=EBI-23845, EBI-23568;
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:14576278}. Mitochondrion inner membrane
CC {ECO:0000269|PubMed:22628554, ECO:0000269|PubMed:22628558}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Grows more slowly in amino acid-free medium (SD).
CC {ECO:0000269|PubMed:22628554}.
CC -!- MISCELLANEOUS: Present with 396 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the mitochondrial pyruvate carrier (MPC) (TC
CC 2.A.105) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Z72602; CAA96785.1; -; Genomic_DNA.
DR EMBL; AY558486; AAS56812.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08025.1; -; Genomic_DNA.
DR PIR; S64087; S64087.
DR RefSeq; NP_011435.1; NM_001180945.1.
DR AlphaFoldDB; P53157; -.
DR BioGRID; 33170; 136.
DR ComplexPortal; CPX-302; Mitochondrial pyruvate carrier, fermentative isoform.
DR ComplexPortal; CPX-303; Mitochondrial pyruvate carrier, respiratory isoform.
DR DIP; DIP-8020N; -.
DR IntAct; P53157; 6.
DR STRING; 4932.YGL080W; -.
DR TCDB; 2.A.105.1.1; the mitochondrial pyruvate carrier (mpc) family.
DR MaxQB; P53157; -.
DR PaxDb; P53157; -.
DR PRIDE; P53157; -.
DR EnsemblFungi; YGL080W_mRNA; YGL080W; YGL080W.
DR GeneID; 852800; -.
DR KEGG; sce:YGL080W; -.
DR SGD; S000003048; MPC1.
DR VEuPathDB; FungiDB:YGL080W; -.
DR eggNOG; KOG1590; Eukaryota.
DR GeneTree; ENSGT00940000171651; -.
DR HOGENOM; CLU_099502_3_2_1; -.
DR InParanoid; P53157; -.
DR OMA; CTTHFWG; -.
DR BioCyc; YEAST:G3O-30581-MON; -.
DR PRO; PR:P53157; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53157; protein.
DR GO; GO:0098800; C:inner mitochondrial membrane protein complex; IDA:ComplexPortal.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0050833; F:pyruvate transmembrane transporter activity; IDA:SGD.
DR GO; GO:0006850; P:mitochondrial pyruvate transmembrane transport; IDA:SGD.
DR InterPro; IPR005336; MPC.
DR Pfam; PF03650; MPC; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..130
FT /note="Mitochondrial pyruvate carrier 1"
FT /id="PRO_0000212805"
FT TRANSMEM 23..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..77
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 130 AA; 14995 MW; 1FD3A5BD176C247C CRC64;
MSQPVQRAAA RSFLQKYINK ETLKYIFTTH FWGPVSNFGI PIAAIYDLKK DPTLISGPMT
FALVTYSGVF MKYALSVSPK NYLLFGCHLI NETAQLAQGY RFLKYTYFTT DEEKKALDKE
WKEKEKTGKQ
