MPC2_HUMAN
ID MPC2_HUMAN Reviewed; 127 AA.
AC O95563; A8K261; Q3SXR6; Q6FIF3;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Mitochondrial pyruvate carrier 2;
DE AltName: Full=Brain protein 44;
GN Name=MPC2; Synonyms=BRP44;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Rhodes S.;
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=11230166; DOI=10.1101/gr.gr1547r;
RA Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S.,
RA Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J.,
RA Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W.,
RA Ottenwaelder B., Obermaier B., Tampe J., Heubner D., Wambutt R., Korn B.,
RA Klein M., Poustka A.;
RT "Towards a catalog of human genes and proteins: sequencing and analysis of
RT 500 novel complete protein coding human cDNAs.";
RL Genome Res. 11:422-435(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=22628558; DOI=10.1126/science.1218099;
RA Bricker D.K., Taylor E.B., Schell J.C., Orsak T., Boutron A., Chen Y.C.,
RA Cox J.E., Cardon C.M., Van Vranken J.G., Dephoure N., Redin C., Boudina S.,
RA Gygi S.P., Brivet M., Thummel C.S., Rutter J.;
RT "A mitochondrial pyruvate carrier required for pyruvate uptake in yeast,
RT Drosophila, and humans.";
RL Science 337:96-100(2012).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: Mediates the uptake of pyruvate into mitochondria.
CC {ECO:0000269|PubMed:22628558}.
CC -!- SUBUNIT: The functional 150 kDa pyruvate import complex is a heteromer
CC of MPC1 and MPC2. {ECO:0000250|UniProtKB:Q9D023}.
CC -!- INTERACTION:
CC O95563; P02654: APOC1; NbExp=3; IntAct=EBI-719403, EBI-1220105;
CC O95563; Q8WUW1: BRK1; NbExp=3; IntAct=EBI-719403, EBI-2837444;
CC O95563; Q9UHD4: CIDEB; NbExp=3; IntAct=EBI-719403, EBI-7062247;
CC O95563; Q9NR28: DIABLO; NbExp=3; IntAct=EBI-719403, EBI-517508;
CC O95563; O00429-3: DNM1L; NbExp=3; IntAct=EBI-719403, EBI-6896746;
CC O95563; Q01658: DR1; NbExp=3; IntAct=EBI-719403, EBI-750300;
CC O95563; P29692-2: EEF1D; NbExp=3; IntAct=EBI-719403, EBI-5280572;
CC O95563; Q9Y5Q9: GTF3C3; NbExp=3; IntAct=EBI-719403, EBI-1054873;
CC O95563; Q5TGZ0: MICOS10; NbExp=3; IntAct=EBI-719403, EBI-12886442;
CC O95563; Q9Y5U8: MPC1; NbExp=3; IntAct=EBI-719403, EBI-721137;
CC O95563; O95563: MPC2; NbExp=3; IntAct=EBI-719403, EBI-719403;
CC O95563; Q6NTF9-3: RHBDD2; NbExp=3; IntAct=EBI-719403, EBI-17589229;
CC O95563; Q13596: SNX1; NbExp=3; IntAct=EBI-719403, EBI-2822329;
CC O95563; Q9NZD8: SPG21; NbExp=3; IntAct=EBI-719403, EBI-742688;
CC O95563; Q17RD7: SYT16; NbExp=3; IntAct=EBI-719403, EBI-10238936;
CC O95563; Q53QW1: TEX44; NbExp=3; IntAct=EBI-719403, EBI-10278496;
CC O95563; O60830: TIMM17B; NbExp=3; IntAct=EBI-719403, EBI-2372529;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:22628558}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:22628558}.
CC -!- SIMILARITY: Belongs to the mitochondrial pyruvate carrier (MPC) (TC
CC 2.A.105) family. {ECO:0000305}.
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DR EMBL; AL035304; CAA22909.1; -; mRNA.
DR EMBL; AL110297; CAB53738.1; -; mRNA.
DR EMBL; AK290126; BAF82815.1; -; mRNA.
DR EMBL; CR407632; CAG28560.1; -; mRNA.
DR EMBL; CR533473; CAG38504.1; -; mRNA.
DR EMBL; Z97876; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471067; EAW90814.1; -; Genomic_DNA.
DR EMBL; BC104157; AAI04158.1; -; mRNA.
DR EMBL; BC104158; AAI04159.1; -; mRNA.
DR CCDS; CCDS1266.1; -.
DR PIR; T14797; T14797.
DR RefSeq; NP_001137146.1; NM_001143674.3.
DR RefSeq; NP_056230.1; NM_015415.3.
DR RefSeq; XP_006711329.1; XM_006711266.3.
DR AlphaFoldDB; O95563; -.
DR SMR; O95563; -.
DR BioGRID; 117389; 55.
DR ComplexPortal; CPX-6154; Mitochondrial pyruvate carrier complex.
DR IntAct; O95563; 32.
DR MINT; O95563; -.
DR STRING; 9606.ENSP00000356820; -.
DR BindingDB; O95563; -.
DR ChEMBL; CHEMBL4295686; -.
DR TCDB; 2.A.105.1.2; the mitochondrial pyruvate carrier (mpc) family.
DR GlyGen; O95563; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; O95563; -.
DR PhosphoSitePlus; O95563; -.
DR SwissPalm; O95563; -.
DR BioMuta; MPC2; -.
DR EPD; O95563; -.
DR jPOST; O95563; -.
DR MassIVE; O95563; -.
DR MaxQB; O95563; -.
DR PaxDb; O95563; -.
DR PeptideAtlas; O95563; -.
DR PRIDE; O95563; -.
DR ProteomicsDB; 50942; -.
DR TopDownProteomics; O95563; -.
DR Antibodypedia; 34345; 216 antibodies from 26 providers.
DR DNASU; 25874; -.
DR Ensembl; ENST00000271373.9; ENSP00000271373.4; ENSG00000143158.11.
DR Ensembl; ENST00000367846.8; ENSP00000356820.4; ENSG00000143158.11.
DR GeneID; 25874; -.
DR KEGG; hsa:25874; -.
DR MANE-Select; ENST00000271373.9; ENSP00000271373.4; NM_001143674.4; NP_001137146.1.
DR UCSC; uc001ges.4; human.
DR CTD; 25874; -.
DR DisGeNET; 25874; -.
DR GeneCards; MPC2; -.
DR HGNC; HGNC:24515; MPC2.
DR HPA; ENSG00000143158; Tissue enhanced (liver).
DR MIM; 614737; gene.
DR neXtProt; NX_O95563; -.
DR OpenTargets; ENSG00000143158; -.
DR PharmGKB; PA142672547; -.
DR VEuPathDB; HostDB:ENSG00000143158; -.
DR eggNOG; KOG1589; Eukaryota.
DR GeneTree; ENSGT00510000047120; -.
DR InParanoid; O95563; -.
DR OMA; KPAWNHA; -.
DR OrthoDB; 1422466at2759; -.
DR PhylomeDB; O95563; -.
DR TreeFam; TF300066; -.
DR PathwayCommons; O95563; -.
DR Reactome; R-HSA-70268; Pyruvate metabolism.
DR SignaLink; O95563; -.
DR BioGRID-ORCS; 25874; 23 hits in 1083 CRISPR screens.
DR ChiTaRS; MPC2; human.
DR GeneWiki; BRP44; -.
DR GenomeRNAi; 25874; -.
DR Pharos; O95563; Tbio.
DR PRO; PR:O95563; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; O95563; protein.
DR Bgee; ENSG00000143158; Expressed in sperm and 207 other tissues.
DR ExpressionAtlas; O95563; baseline and differential.
DR Genevisible; O95563; HS.
DR GO; GO:0098800; C:inner mitochondrial membrane protein complex; IC:ComplexPortal.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:ComplexPortal.
DR GO; GO:0005739; C:mitochondrion; IDA:LIFEdb.
DR GO; GO:0005634; C:nucleus; HDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0050833; F:pyruvate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0061732; P:mitochondrial acetyl-CoA biosynthetic process from pyruvate; IEA:Ensembl.
DR GO; GO:0006850; P:mitochondrial pyruvate transmembrane transport; IBA:GO_Central.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Ensembl.
DR InterPro; IPR005336; MPC.
DR Pfam; PF03650; MPC; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..127
FT /note="Mitochondrial pyruvate carrier 2"
FT /id="PRO_0000212793"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..115
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 127 AA; 14279 MW; D82E5879AB2EEA68 CRC64;
MSAAGARGLR ATYHRLLDKV ELMLPEKLRP LYNHPAGPRT VFFWAPIMKW GLVCAGLADM
ARPAEKLSTA QSAVLMATGF IWSRYSLVII PKNWSLFAVN FFVGAAGASQ LFRIWRYNQE
LKAKAHK
