MPC2_MOUSE
ID MPC2_MOUSE Reviewed; 127 AA.
AC Q9D023; Q3UA44;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Mitochondrial pyruvate carrier 2;
DE AltName: Full=Brain protein 44;
GN Name=Mpc2; Synonyms=Brp44;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Embryo, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=22628554; DOI=10.1126/science.1218530;
RA Herzig S., Raemy E., Montessuit S., Veuthey J.L., Zamboni N.,
RA Westermann B., Kunji E.R., Martinou J.C.;
RT "Identification and functional expression of the mitochondrial pyruvate
RT carrier.";
RL Science 337:93-96(2012).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-26, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23576753; DOI=10.1073/pnas.1302961110;
RA Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B.,
RA Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.;
RT "Label-free quantitative proteomics of the lysine acetylome in mitochondria
RT identifies substrates of SIRT3 in metabolic pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013).
CC -!- FUNCTION: Mediates the uptake of pyruvate into mitochondria.
CC {ECO:0000269|PubMed:22628554}.
CC -!- SUBUNIT: The functional 150 kDa pyruvate import complex is a heteromer
CC of MPC1 and MPC2. {ECO:0000269|PubMed:22628554}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:22628554}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:22628554}.
CC -!- SIMILARITY: Belongs to the mitochondrial pyruvate carrier (MPC) (TC
CC 2.A.105) family. {ECO:0000305}.
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DR EMBL; AK002233; BAC24983.1; -; mRNA.
DR EMBL; AK011889; BAB27898.1; -; mRNA.
DR EMBL; AK151522; BAE30471.1; -; mRNA.
DR EMBL; BC018324; AAH18324.1; -; mRNA.
DR CCDS; CCDS15442.1; -.
DR RefSeq; NP_081706.1; NM_027430.2.
DR AlphaFoldDB; Q9D023; -.
DR SMR; Q9D023; -.
DR BioGRID; 214064; 26.
DR IntAct; Q9D023; 3.
DR MINT; Q9D023; -.
DR STRING; 10090.ENSMUSP00000027853; -.
DR GuidetoPHARMACOLOGY; 3023; -.
DR iPTMnet; Q9D023; -.
DR PhosphoSitePlus; Q9D023; -.
DR EPD; Q9D023; -.
DR jPOST; Q9D023; -.
DR MaxQB; Q9D023; -.
DR PaxDb; Q9D023; -.
DR PeptideAtlas; Q9D023; -.
DR PRIDE; Q9D023; -.
DR ProteomicsDB; 291485; -.
DR Antibodypedia; 34345; 216 antibodies from 26 providers.
DR Ensembl; ENSMUST00000027853; ENSMUSP00000027853; ENSMUSG00000026568.
DR GeneID; 70456; -.
DR KEGG; mmu:70456; -.
DR UCSC; uc007djd.1; mouse.
DR CTD; 25874; -.
DR MGI; MGI:1917706; Mpc2.
DR VEuPathDB; HostDB:ENSMUSG00000026568; -.
DR eggNOG; KOG1589; Eukaryota.
DR GeneTree; ENSGT00510000047120; -.
DR HOGENOM; CLU_099502_4_1_1; -.
DR InParanoid; Q9D023; -.
DR OMA; KPAWNHA; -.
DR OrthoDB; 1422466at2759; -.
DR PhylomeDB; Q9D023; -.
DR TreeFam; TF300066; -.
DR BioGRID-ORCS; 70456; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Mpc2; mouse.
DR PRO; PR:Q9D023; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9D023; protein.
DR Bgee; ENSMUSG00000026568; Expressed in metanephric proximal tubule and 246 other tissues.
DR ExpressionAtlas; Q9D023; baseline and differential.
DR Genevisible; Q9D023; MM.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IBA:GO_Central.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0050833; F:pyruvate transmembrane transporter activity; IGI:MGI.
DR GO; GO:0061732; P:mitochondrial acetyl-CoA biosynthetic process from pyruvate; IMP:MGI.
DR GO; GO:0006850; P:mitochondrial pyruvate transmembrane transport; IGI:MGI.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IMP:MGI.
DR InterPro; IPR005336; MPC.
DR Pfam; PF03650; MPC; 1.
PE 1: Evidence at protein level;
KW Acetylation; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..127
FT /note="Mitochondrial pyruvate carrier 2"
FT /id="PRO_0000212794"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOD_RES 26
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23576753"
SQ SEQUENCE 127 AA; 14286 MW; BE8AEC9DF74405A6 CRC64;
MAAAGARGLR ATYHRLMDKV ELLLPKKLRP LYNHPAGPRT VFFWAPIMKW GLVCAGLADM
ARPAEKLSTA QSTVLMATGF IWSRYSLVII PKNWSLFAVN FFVGSAGASQ LFRIWRYNQE
LKSKGIQ
