MPC2_YEAST
ID MPC2_YEAST Reviewed; 129 AA.
AC P38857; D3DLB1;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Mitochondrial pyruvate carrier 2 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
DE Short=MPC2 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
GN Name=MPC2 {ECO:0000303|PubMed:22628554, ECO:0000303|PubMed:22628558};
GN OrderedLocusNames=YHR162W {ECO:0000312|SGD:S000001205};
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=22628554; DOI=10.1126/science.1218530;
RA Herzig S., Raemy E., Montessuit S., Veuthey J.L., Zamboni N.,
RA Westermann B., Kunji E.R., Martinou J.C.;
RT "Identification and functional expression of the mitochondrial pyruvate
RT carrier.";
RL Science 337:93-96(2012).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=22628558; DOI=10.1126/science.1218099;
RA Bricker D.K., Taylor E.B., Schell J.C., Orsak T., Boutron A., Chen Y.C.,
RA Cox J.E., Cardon C.M., Van Vranken J.G., Dephoure N., Redin C., Boudina S.,
RA Gygi S.P., Brivet M., Thummel C.S., Rutter J.;
RT "A mitochondrial pyruvate carrier required for pyruvate uptake in yeast,
RT Drosophila, and humans.";
RL Science 337:96-100(2012).
CC -!- FUNCTION: Mediates the uptake of pyruvate into mitochondria.
CC {ECO:0000269|PubMed:22628554, ECO:0000269|PubMed:22628558}.
CC -!- SUBUNIT: The functional 150 kDa pyruvate import complex is a heteromer
CC of MPC1 and either MPC2 or MPC3. {ECO:0000269|PubMed:22628554,
CC ECO:0000269|PubMed:22628558}.
CC -!- INTERACTION:
CC P38857; P53157: MPC1; NbExp=9; IntAct=EBI-24818, EBI-23845;
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095}.
CC Mitochondrion inner membrane {ECO:0000269|PubMed:22628558}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- INDUCTION: Mainly expressed when grown on glucose containing growth
CC medium. {ECO:0000269|PubMed:22628554}.
CC -!- DISRUPTION PHENOTYPE: Grows more slowly in amino acid-free medium (SD),
CC when combined with disruption of MPC3. {ECO:0000269|PubMed:22628554}.
CC -!- MISCELLANEOUS: Present with 3410 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the mitochondrial pyruvate carrier (MPC) (TC
CC 2.A.105) family. {ECO:0000305}.
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DR EMBL; U00027; AAB68009.1; -; Genomic_DNA.
DR EMBL; AY558303; AAS56629.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06855.1; -; Genomic_DNA.
DR PIR; S48902; S48902.
DR RefSeq; NP_012032.1; NM_001179293.1.
DR AlphaFoldDB; P38857; -.
DR SMR; P38857; -.
DR BioGRID; 36596; 87.
DR ComplexPortal; CPX-302; Mitochondrial pyruvate carrier, fermentative isoform.
DR DIP; DIP-8092N; -.
DR IntAct; P38857; 8.
DR STRING; 4932.YHR162W; -.
DR TCDB; 2.A.105.1.1; the mitochondrial pyruvate carrier (mpc) family.
DR MaxQB; P38857; -.
DR PaxDb; P38857; -.
DR PRIDE; P38857; -.
DR EnsemblFungi; YHR162W_mRNA; YHR162W; YHR162W.
DR GeneID; 856567; -.
DR KEGG; sce:YHR162W; -.
DR SGD; S000001205; MPC2.
DR VEuPathDB; FungiDB:YHR162W; -.
DR eggNOG; KOG1589; Eukaryota.
DR GeneTree; ENSGT00510000047120; -.
DR HOGENOM; CLU_099502_1_0_1; -.
DR InParanoid; P38857; -.
DR OMA; KPAWNHA; -.
DR BioCyc; YEAST:G3O-31197-MON; -.
DR PRO; PR:P38857; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38857; protein.
DR GO; GO:0098800; C:inner mitochondrial membrane protein complex; IDA:ComplexPortal.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0050833; F:pyruvate transmembrane transporter activity; IDA:SGD.
DR GO; GO:0006850; P:mitochondrial pyruvate transmembrane transport; IDA:SGD.
DR InterPro; IPR005336; MPC.
DR Pfam; PF03650; MPC; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..129
FT /note="Mitochondrial pyruvate carrier 2"
FT /id="PRO_0000212801"
FT TRANSMEM 23..39
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 55..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..91
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 129 AA; 14555 MW; 5C1FB3A463DE24A7 CRC64;
MSTSSVRFAF RRFWQSETGP KTVHFWAPTL KWGLVFAGFS DMKRPVEKIS GAQNLSLLST
ALIWTRWSFV IKPRNILLAS VNSFLCLTAG YQLGRIANYR IRNGDSISQL CSYILSGADE
SKKEITTGR
