MPC3_ARATH
ID MPC3_ARATH Reviewed; 108 AA.
AC Q8LD38; Q84VZ9; Q9M0U5;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Mitochondrial pyruvate carrier 3 {ECO:0000305};
DE AltName: Full=Protein NEGATIVE REGULATOR OF GUARD CELL ABA SIGNALING 1 {ECO:0000303|PubMed:24842572};
GN Name=MPC3 {ECO:0000305};
GN Synonyms=DiDi 19A-1b {ECO:0000303|PubMed:11277426},
GN NRGA1 {ECO:0000303|PubMed:24842572};
GN OrderedLocusNames=At4g05590 {ECO:0000312|Araport:AT4G05590};
GN ORFNames=F6H8.10 {ECO:0000312|EMBL:AF178045};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAM64304.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=11277426; DOI=10.1094/mpmi.2001.14.3.288;
RA Vercauteren I., van der Schueren E., Van Montagu M., Gheysen G.;
RT "Arabidopsis thaliana genes expressed in the early compatible interaction
RT with root-knot nematodes.";
RL Mol. Plant Microbe Interact. 14:288-299(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia; TISSUE=Flower, and Silique;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY,
RP AND INDUCTION.
RX PubMed=24842572; DOI=10.1093/mp/ssu061;
RA Li C.L., Wang M., Ma X.Y., Zhang W.;
RT "NRGA1, a putative mitochondrial pyruvate carrier, mediates ABA regulation
RT of guard cell ion channels and drought stress responses in Arabidopsis.";
RL Mol. Plant 7:1508-1521(2014).
CC -!- FUNCTION: Mediates the uptake of pyruvate into mitochondria. Negatively
CC regulates ABA-induced guard cell signaling and mediates drought stress
CC responses. {ECO:0000269|PubMed:24842572}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:24842572}.
CC Mitochondrion inner membrane {ECO:0000250|UniProtKB:P53157}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8LD38-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8LD38-2; Sequence=VSP_057338;
CC -!- TISSUE SPECIFICITY: Abundant in leaf and particularly in the guard
CC cells. {ECO:0000269|PubMed:24842572}.
CC -!- INDUCTION: By abscisic acid (ABA) and drought stress.
CC {ECO:0000269|PubMed:24842572}.
CC -!- DISRUPTION PHENOTYPE: Abscisic acid (ABA) hypersensitivity of stomatal
CC movements and enhanced drought tolerance.
CC {ECO:0000269|PubMed:24842572}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the mitochondrial pyruvate carrier (MPC) (TC
CC 2.A.105) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB77923.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ276762; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AF178045; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL161504; CAB77923.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE82535.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82536.1; -; Genomic_DNA.
DR EMBL; BT004584; AAO42830.1; -; mRNA.
DR EMBL; AK227500; BAE99500.1; -; mRNA.
DR EMBL; AK317025; BAH19719.1; -; mRNA.
DR EMBL; AY086228; AAM64304.1; -; mRNA.
DR PIR; C85070; C85070.
DR RefSeq; NP_001031587.1; NM_001036510.3. [Q8LD38-2]
DR RefSeq; NP_567306.1; NM_116797.4. [Q8LD38-1]
DR AlphaFoldDB; Q8LD38; -.
DR SMR; Q8LD38; -.
DR TCDB; 2.A.105.1.4; the mitochondrial pyruvate carrier (mpc) family.
DR PRIDE; Q8LD38; -.
DR ProteomicsDB; 239064; -. [Q8LD38-1]
DR EnsemblPlants; AT4G05590.1; AT4G05590.1; AT4G05590. [Q8LD38-1]
DR EnsemblPlants; AT4G05590.2; AT4G05590.2; AT4G05590. [Q8LD38-2]
DR GeneID; 825927; -.
DR Gramene; AT4G05590.1; AT4G05590.1; AT4G05590. [Q8LD38-1]
DR Gramene; AT4G05590.2; AT4G05590.2; AT4G05590. [Q8LD38-2]
DR KEGG; ath:AT4G05590; -.
DR Araport; AT4G05590; -.
DR TAIR; locus:2126583; AT4G05590.
DR HOGENOM; CLU_099502_1_2_1; -.
DR OMA; YASETDH; -.
DR OrthoDB; 1422466at2759; -.
DR PhylomeDB; Q8LD38; -.
DR PRO; PR:Q8LD38; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8LD38; baseline and differential.
DR Genevisible; Q8LD38; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0006850; P:mitochondrial pyruvate transmembrane transport; IMP:UniProtKB.
DR GO; GO:0010360; P:negative regulation of anion channel activity; IMP:TAIR.
DR GO; GO:1901017; P:negative regulation of potassium ion transmembrane transporter activity; IMP:TAIR.
DR GO; GO:2000070; P:regulation of response to water deprivation; IMP:TAIR.
DR InterPro; IPR005336; MPC.
DR Pfam; PF03650; MPC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..108
FT /note="Mitochondrial pyruvate carrier 3"
FT /id="PRO_0000431617"
FT TRANSMEM 19..35
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 51..67
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 74..90
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 51
FT /note="I -> IGIILTIGLSYLYSAHIAVMYRCVNFNVYMMPRMAVIVS (in
FT isoform 2)"
FT /id="VSP_057338"
SQ SEQUENCE 108 AA; 12181 MW; 59C1805098CFBFA8 CRC64;
MATSKLQALW NHPAGPKTIH FWAPTFKWGI SIANIADFQK PPETLSYPQQ IVITGTGLVW
SRYSTVITPK NWNLFSVSLG MAVTGIYQLT RKIKHDYVYE ANSIVAKE
